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From 2012.igem.org

Attributions

Many thanks to all those who helped us, advised us and encouraged us over the summer !

The team

The team is composed of a mix of students, from L2 (2nd year after baccalaureate) to M2 (5th year after baccalaureate). We worked together from mid june to the end of september, some full time, some part time. More details can be found on the team page.

Funding and administrative management

The team did most of the fundraising work, creating a sponsoring file, contacting potential sponsors and presenting the project. We managed the money ourselves, through Genopole and through a "1901 law" association. Genopole supported our project, paying inscription and jamboree participation fees, as well as donating 5000 euros towards the project. Evry university contributed 4800 euros. Sanofi donated 10 000 euros, and the French embassy in the United States donated 1500 euros. Our other sponsors helped us by providing discounts or free samples.

We would like thank Jean-Loup Faulon for his help with the Sanofi sponsoring, and Dominique Zeliszewski for her help with team organisation and with the preparation of the sponsoring file. Many thanks to Joan Hérisson who helped with setting up the iGEM lab in the iSSB synthetic biology platform, and to Maelle Cochennec who managed the money we kept with Genopole. We would also like to thank a thousand times Sylvie Bobelet and Bernadette Lauret, without whom we would never have survived the summer, for all the help they provided in the administrative organisation of the team, and acting as a relay between us and Genopole.

Finally, we have surely missed out many people without whom we could not have completed the project, so we would like to adress a general thanks to all the people in iSSB who encouraged and supported us over the summer

Cloning

All cloning work was done by the team in Institute of Systems & Synthetic Biology, Evry. Supervisor Thomas Landrain gave advice on cloning stategy throughout the project.

Animal work

Adult frogs are kept in the animal house shared between the Metamorphosys team of iSSB and Watchfrog (a company). The adult frogs were handled by Nicolas Pollet's team (for HCG injection, egg and sperm recovery). For the auxin toxicity tests, auxin penetration experiments and the E. coli to Xenopus communication devices, the fertilised eggs were provided by the Metamophosys team of the iSSB. For all tests of biobricks in Xenopus , the team was provided with unfertilised eggs and sperm, and in vitro fertilisation and DNA injection were done by team members under the supervision of Nicolas Pollet. Eggs were sorted and kept and analysed by the team, in the Metamorphosys labs.

Microscopy

Most microscopy photos were taken in the Metamorphosys labs by the team, with the help and supervision of Nicolas Pollet, Aurore Thélie and Léna Vouillot (Postdoc and PhD student in Metamorphosys team). We would like to thank Dr. Daniel Stockholm and Genethon for letting us use LSM 510 META Laser Scanning Microscope from Zeiss, which was used with his help and that of Nicolas Pollet and Aurore Thélie for high definition fluorescence images.

HPLC

Many thanks to Damien Baud (Genoscope, Evry), who run our samples on HPLC, and to our advisor Anna who helped us analyse the spectra.