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Team:Marburg SYNMIKRO/Primer
From 2012.igem.org
Revision as of 12:35, 26 September 2012 by Enantiomere (Talk | contribs)
Primer
| Primer | Name | Sequence | Length | G/C content | TM |
|---|---|---|---|---|---|
| MS1 | AmiC_ss_std_10_fwd | 5'-GCGAATTCGCGGCCGCTTCTAGATGTCAGGATCCAACA-3' | xx bp | yy % | zz °C |
| MS2 | AmiC_ss_std_10_rev | 5'-GCCTGCAGCGGCCGCTACTAGTAGCCAGACTGACCTGA-3' | xx bp | yy % | zz °C |
| MS3 | HU_std_10_fwd | 5'-GCGAATTCGCGGCCGCTTCTAGATGAACAAGACTCAA-3' | 16.07.12 | ||
| MS4 | HU_std_10_rev | 5'-GCCTGCAGCGGCCGCTACTAGTCTTAACTGCGTCTTTCv | 16.07.12 | ||
| MS4b | HU_std_10_rev2 | 5'-GCCTGCAGCGGCCGCTACTAGTACCCTTAACTGCGTCTTTCAGTG-3' | 10.08.2012 | ||
| MS5 | GroEs_std_10_fwd | 5'-GCTTCTAGATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAA CTAAATCTGCTGGCGGCATCGTTCTGACCGGCTCTGCGGCGGCTAAATCCACCCGCG-3' | 16.07.12 | ||
| MS6 | GroEs_std_10_rev | 5'-GCCTGCAGCGGCCGCTACTAGTATTACGCTTCAACAAT-3' | 16.07.12 | ||
| MS6b | GroES_std_10_rev2 | 5'-GCCTGCAGCGGCCGCTACTAGTACCCGCTTCAACAATTGCCAGAA-3' | 10.08.2012 | ||
| MS7 | GixR_std_10_fwd | 5'-CTAGAGCGTTATCCAAAACCTCGGTTTACAGGAATACTAGTAGCGGCCGCTGCA-3' | 16.07.12 | ||
| MS8 | GixR_std_10_rev | 5'-GCGGCCGCTACTAGTATTCCTGTAAACCGAGGTTTTGGATAACGCT-3' | 16.07.12 | ||
| MS9 | Enhancer_std_10_fwd | 5'-GCGAATTCGCGGCCGCTTCTAGAGGGGTATCAACAAATGACC-3' | 16.07.12 | ||
| MS10 | Enhancer_std_10_rev | 5'-GCCTGCAGCGGCCGCTACTAGTATTTGTTCACATCCTGCAC-3' | 16.07.12 | ||
| MS11 | GIN_std_10_fwd | 5'-GCGAATTCGCGGCCGCTTCTAGATGCTGATTGGCTATGTAAGGGTATCAACA-3' | 20.07.12 | ||
| MS12 | RBS + GIN_std_10_fwd | 5'-GCGAATTCGCGGCCGCTTCTAGAGAAAGAGGGGACAAAC TAGATGCTGATTGGCTATGTAAGGGTATCAACA-3' | 20.07.12 | ||
| MS13 | GIN_std_10_rev | 5'-GCCTGCAGCGGCCGCTACTAGTATTATTAATTGATTCGATCGTCGTTTTCTATATGCGC-3' | 20.07.12 | ||
| MS14 | RFP_std_10_fwd | 5'-GCGAATTCGCGGCCGCTTCTAGAGCTTCCTCCGAAGACGTT-3' | 20.07.12 | ||
| MS15 | RFP_std_10_rev | 5'-GCCTGCAGCGGCCGCTACTAGTATTATTAAGCACCGGTGGA-3' | 20.07.12 | ||
| MS16 | CFP_std_10_fwd | 5'-GCGAATTCGCGGCCGCTTCTAGAGTGAGCAAGGGCGAGGAGCT-3' | 20.07.12 | ||
| MS17 | CFP_std_10_rev | 5'-GCCTGCAGCGGCCGCTACTAGTATTATTACTTGTACAGCTCGT-3' | 20.07.12 | ||
| MS18 | Prefix_std_10_fwd | 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAG-3' | 10.08.2012 | ||
| MS19 | Suffix_std_10_rev | 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTA-3' | 10.08.2012 | ||
| MS20 | VF2_std_10_fwd | 5'-TGCCACCTGACGTCTAAGAA-3' | 10.08.2012 | ||
| MS21 | VR_std_10_rev | 5'-ATTACCGCCTTTGAGTGAGC-3' | 10.08.2012 | ||
| MS22 | SB-prep-3P-1 | 5'-GCCGCTGCAGTCCGGCAAAAAA-3' | 20.08.2012 | ||
| MS23 | SB-prep-2Ea | 5'-ATGAATTCCAGAAATCATCCTTAGCG-3' | 20.08.2012 | ||
| MS24 | Suffix_std_10_fwd | 5'-ACTAGTAGCGGCCGCTGCAG-3' | 20.08.2012 | ||
| MS25 | Prefix_std_10_rev | 5'-TCTAGATGCGGCCGCGAATTC-3' | 20.08.2012 | ||
| MS26 | SacB_std_10_fwd | 5'-CGAATTCGCGGCCGCTTCTAGATGAACATCAAAAAGTTTGC-3' | 20.08.2012 | ||
| MS27 | SacB_std_10_rev | 5'-GGCTGCAGCGGCCGCTACTAGTATTATTATTTGTTAACTG-3' | 20.08.2012 |
