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Team:Marburg SYNMIKRO/Primer
From 2012.igem.org
Primer
| No. | Name | Sequence | Length | G/C content | TM |
|---|---|---|---|---|---|
| MS1 | AmiC_ss_std_10_fwd | 5'-GCGAATTCGCGGCCGCTTCTAGATGTCAGGATCCAACA-3' | 38 bp | 55.2 % | 86.5 °C |
| MS2 | AmiC_ss_std_10_rev | 5'-GCCTGCAGCGGCCGCTACTAGTAGCCAGACTGACCTGA-3' | 38 bp | 63.1 % | 85.5 °C |
| MS3 | HU_std_10_fwd | 5'-GCGAATTCGCGGCCGCTTCTAGATGAACAAGACTCAA-3' | 37 bp | 51.3 % | 83.6 °C |
| MS4 | HU_std_10_rev | 5'-GCCTGCAGCGGCCGCTACTAGTCTTAACTGCGTCTTTCv | 38 bp | 57.8 % | 82.8 °C |
| MS4b | HU_std_10_rev2 | 5'-GCCTGCAGCGGCCGCTACTAGTACCCTTAACTGCGTCTTTCAGTG-3' | 45 bp | 57.7 % | 85.7 °C |
| MS5 | GroEs_std_10_fwd | 5'-GCTTCTAGATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAA CTAAATCTGCTGGCGGCATCGTTCTGACCGGCTCTGCGGCGGCTAAATCCACCCGCG-3' | 120 bp | 51.6 % | 98.0 °C |
| MS6 | GroEs_std_10_rev | 5'-GCCTGCAGCGGCCGCTACTAGTATTACGCTTCAACAAT-3' | 45 bp | 57.7 % | 88.7 °C |
| MS6b | GroES_std_10_rev2 | 5'-GCCTGCAGCGGCCGCTACTAGTACCCGCTTCAACAATTGCCAGAA-3' | 38 bp | 52.6 % | 81.4 °C |
| MS7 | GixR_std_10_fwd | 5'-CTAGAGCGTTATCCAAAACCTCGGTTTACAGGAATACTAGTAGCGGCCGCTGCA-3' | 54 bp | 50.0 % | 85.1 °C |
| MS8 | GixR_std_10_rev | 5'-GCGGCCGCTACTAGTATTCCTGTAAACCGAGGTTTTGGATAACGCT-3' | 46 bp | 50.0 % | 82.7 °C |
| MS9 | Enhancer_std_10_fwd | 5'-GCGAATTCGCGGCCGCTTCTAGAGGGGTATCAACAAATGACC-3' | 42 bp | 54.7 % | 86.2 °C |
| MS10 | Enhancer_std_10_rev | 5'-GCCTGCAGCGGCCGCTACTAGTATTTGTTCACATCCTGCAC-3' | 41 bp | 56.0 % | 85.1 °C |
| MS11 | GIN_std_10_fwd | 5'-GCGAATTCGCGGCCGCTTCTAGATGCTGATTGGCTATGTAAGGGTATCAACA-3' | 52 bp | 50.0 % | 87.6 °C |
| MS12 | RBS + GIN_std_10_fwd | 5'-GCGAATTCGCGGCCGCTTCTAGAGAAAGAGGGGACAAAC TAGATGCTGATTGGCTATGTAAGGGTATCAACA-3' | 72 bp | 48.8 % | 89.8 °C |
| MS13 | GIN_std_10_rev | 5'-GCCTGCAGCGGCCGCTACTAGTATTATTAATTGATTCGATCGTCGTTTTCTATATGCGC-3' | 59 bp | 45.7 % | 85.5 °C |
| MS14 | RFP_std_10_fwd | 5'-GCGAATTCGCGGCCGCTTCTAGAGCTTCCTCCGAAGACGTT-3' | 41 bp | 58.5 % | 87.6 °C |
| MS15 | RFP_std_10_rev | 5'-GCCTGCAGCGGCCGCTACTAGTATTATTAAGCACCGGTGGA-3' | 41 bp | 56.0 % | 84.2 °C |
| MS16 | CFP_std_10_fwd | 5'-GCGAATTCGCGGCCGCTTCTAGAGTGAGCAAGGGCGAGGAGCT-3' | 43 bp | 62.7 % | 90.3 °C |
| MS17 | CFP_std_10_rev | 5'-GCCTGCAGCGGCCGCTACTAGTATTATTACTTGTACAGCTCGT-3' | 43 bp | 51.1 % | 80.5 °C |
| MS18 | Prefix_std_10_fwd | 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAG-3' | 28 bp | 53.5 % | 77.0 °C |
| MS19 | Suffix_std_10_rev | 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTA-3' | 29 bp | 55.1 % | 75.3 °C |
| MS20 | VF2_std_10_fwd | 5'-TGCCACCTGACGTCTAAGAA-3' | 20 bp | 50.0 % | 63.5 °C |
| MS21 | VR_std_10_rev | 5'-ATTACCGCCTTTGAGTGAGC-3' | 20 bp | 50 % | 63.1 °C |
| MS22 | SB-prep-3P-1 | 5'-GCCGCTGCAGTCCGGCAAAAAA-3' | 22 bp | 59.0 % | 77.2 °C |
| MS23 | SB-prep-2Ea | 5'-ATGAATTCCAGAAATCATCCTTAGCG-3' | 26 bp | 38.4 % | 67.6 °C |
| MS24 | Suffix_std_10_fwd | 5'-ACTAGTAGCGGCCGCTGCAG-3' | 20 bp | 65.0 % | 68.8 °C |
| MS25 | Prefix_std_10_rev | 5'-TCTAGATGCGGCCGCGAATTC-3' | 21 bp | 57.1 % | 72.2 °C |
| MS26 | SacB_std_10_fwd | 5'-CGAATTCGCGGCCGCTTCTAGATGAACATCAAAAAGTTTGC-3' | 42 bp | 47.6 % | 84.8 °C |
| MS27 | SacB_std_10_rev | 5'-GGCTGCAGCGGCCGCTACTAGTATTATTATTTGTTAACTG-3' | 40 bp | 45.0 % | 76.8 °C |
