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From 2012.igem.org

Results

Contents 1 Results 1.1 Parts Statuses 1.2 Identified Problems 1.3 Progress Illustraion

Parts Statuses

• Pfet3, Panb1, lacZ: PCR was not successful for many tries. For Pfet3, new primers were designed but there was no time left to complete this part.
• Tadh1: Tadh1 has been successfully amplified and ligation in pGEM vectors is under way.
• pRS313, pRS315, pRS316: The shuttle plasmids are available and digested.
• Padh1, Psuc2, rox1, mig1, luciferase, mPR Danio rerio, mPR Xenopus laevis: These parts are all available and digested. The ligation in both pSB1C3 and our pRS shuttle plasmids was so far not successful.

Identified Problems

• PCR problems: Some PCRs yielded only products in very low concentrations. Some primers also amplified multiple fragments. New primers partially solved these troubles.

Progress Illustraion

The following illustraion gives a quick overview of the final status of our lab work.