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From 2012.igem.org

Contents 1 Results 1.1 Plug and Apply System 1.2 Outside Modifications 1.3 Inside Modifications 1.4 Virus Like particles 1.5 General

Results

Plug and Apply System

GFP-coil, coils, the idea

Outside Modifications

• Designed the primers for amplification of the needed constructs
• Delivered the proof of principle that these primers work [link to framshift, Hugo]
• Provided the templates for modifications and placed them in the partsregistry
• Isolated the PLRV from nature which, in theory, should be easy to modify on the outside

Inside Modifications

Negative CCMV, Frameshift

Virus Like particles

Detection Isolation from nature

• RNA isolation
• RT-PCR
• Addition of prefix and suffix

General

• We organised a mini Symposium for all dutch teams in the begining of the competition to create a national network and eventualy a national effort
• We visited secondary schools to teach them about synthetic biology and let the make leafelets about synthetic biology.
• We went to present our project and The Constructor at the Centre of Advanced Studies Synthetic Biology conference in Munich, where we learned a lot and get familiar with the German iGEM teams
• We communicated to a graphical designer to obtain an kinetic graphical movie of our VLP formation, that is included in our introduction page movie.
• We involved a communication study in our discovery festival human practises, so that we do not only inform the public, but also will have results on how scientist communicate with the public.
• We have taken a number of inverviews with stakeholders to have an opinion of expert on our project, regulations around our project and about the idea of an open source environment