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Team:Costa Rica-TEC-UNA/Project
From 2012.igem.org
Overall project
Cibus 3.0 takes biodiesel production to a new level using dairy industry wastes. Annually, about 675 thousand tons of whey are thrown into rivers. This because at the present time there isn’t a program for reusing this waste, and producers find it difficult to treat them properly because of its chemical composition.
Our idea consists in the modification of two bacteria: Rhodococcus opacus and Escherichia coli, both maintained in whey based medium. Overexpression of the natural triglycerides (TGA) producing ability of R. opacus is achieved by inserting an optimized sequence of a DGA acyltransferase gene, constitutively expressed, and an inducible “suicide device” in order to extract them with ease.
On the other hand, E. coli is transformed with an optimized sequence of a lipase from B. cepacia which is secreted to the medium where we extract it continuously and encapsulate it. Now all what it takes to finish the job is adding our encapsulated enzymes to the extracted TGAs and mixing them with some ethanol to obtain our biodiesel!
Project Details
The Experiments
Results
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