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Team:Marburg SYNMIKRO/Primer
From 2012.igem.org
Revision as of 22:04, 25 September 2012 by Enantiomere (Talk | contribs)
Primer
| Primer | Name | Sequence | Length | G/C content | TM |
|---|---|---|---|---|---|
| MS1 | AmiC_ss_std_10_fwd | 5'-GCGAATTCGCGGCCGCTTCTAGATGTCAGGATCCAACA-3' | xx bp | yy % | zz °C |
| MS2 | AmiC_ss_std_10_rev | 5'-GCCTGCAGCGGCCGCTACTAGTAGCCAGACTGACCTGA-3' | xx bp | yy % | zz °C |
| I A3 | MS3 HU_std_10_fwd | 5'-GCGAATTCGCGGCCGCTTCTAGATGAACAAGACTCAA | 16.07.12 | ||
| I A4 | MS4 HU_std_10_rev | 5'-GCCTGCAGCGGCCGCTACTAGTCTTAACTGCGTCTTTC | 16.07.12 | ||
| I A5 | MS5 GroEs_std_10_fwd | 5'-GCTTCTAGATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAA CTAAATCTGCTGGCGGCATCGTTCTGACCGGCTCTGCGGCGGCTAAATCCACCCGCG | 16.07.12 | ||
| I A6 | MS6 GroEs_std_10_rev | 5'-GCCTGCAGCGGCCGCTACTAGTATTACGCTTCAACAAT | 16.07.12 | ||
| I A7 | MS7 GixR_std_10_fwd | 5'-CTAGAGCGTTATCCAAAACCTCGGTTTACAGGAATACTAGTAGCGGCCGCTGCA | 16.07.12 | ||
| I A8 | MS8 GixR_std_10_rev | 5'-GCGGCCGCTACTAGTATTCCTGTAAACCGAGGTTTTGGATAACGCT | 16.07.12 | ||
| I A9 | MS9 Enhancer_std_10_fwd | 5'-GCGAATTCGCGGCCGCTTCTAGAGGGGTATCAACAAATGACC | 16.07.12 | ||
| I B1 | MS10 Enhancer_std_10_rev | 5'-GCCTGCAGCGGCCGCTACTAGTATTTGTTCACATCCTGCAC | 16.07.12 | ||
| I B2 | MS11 GIN_std_10_fwd | 5'-GCGAATTCGCGGCCGCTTCTAGATGCTGATTGGCTATGTAAGGGTATCAACA | 20.07.12 | ||
| I B3 | MS12 RBS + GIN_std_10_fwd | 5'-GCGAATTCGCGGCCGCTTCTAGAGAAAGAGGGGACAAAC TAGATGCTGATTGGCTATGTAAGGGTATCAACA | 20.07.12 | ||
| I B4 | MS13 GIN_std_10_rev | 5'-GCCTGCAGCGGCCGCTACTAGTATTATTAATTGATTCGATCGTCGTTTTCTATATGCGC | 20.07.12 | ||
| I B5 | MS14 RFP_std_10_fwd | 5'-GCGAATTCGCGGCCGCTTCTAGAGCTTCCTCCGAAGACGTT | 20.07.12 | ||
| I B6 | MS15 RFP_std_10_rev | 5'-GCCTGCAGCGGCCGCTACTAGTATTATTAAGCACCGGTGGA | 20.07.12 | ||
| I B7 | MS16 CFP_std_10_fwd | 5'-GCGAATTCGCGGCCGCTTCTAGAGTGAGCAAGGGCGAGGAGCT | 20.07.12 | ||
| I B8 | MS17 CFP_std_10_rev | 5'-GCCTGCAGCGGCCGCTACTAGTATTATTACTTGTACAGCTCGT | 20.07.12 | ||
| I B9 | MS 18 Prefix_std_10_fwd | 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAG | 10.08.2012 | ||
| I C1 | MS 19 Suffix_std_10_rev | 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTA | 10.08.2012 | ||
| I C2 | MS 20 VF2_std_10_fwd | 5'-TGCCACCTGACGTCTAAGAA | 10.08.2012 | ||
| I C3 | MS 21 VR_std_10_rev | 5'-ATTACCGCCTTTGAGTGAGC | 10.08.2012 | ||
| I C4 | Ms22ccdB fwd | 5'-CCGAATTCGCGGCCGCTTCTAGAGGGCTTACTAAAAGCCAGATA | 10.08.2012 | ||
| I C5 | Ms22ccdB Rev | 5'-CCCTGCAGCGGCCGCTACTAGTATTATTATATTCCCCAGAACA | 10.08.2012 | ||
| I C6 | MS4 (b) HU_sTd_10_rev | 5'-GCCTGCAGCGGCCGCTACTAGTACCCTTAACTGCGTCTTTCAGTG | 10.08.2012 | ||
| I C7 | MS6 (b) GroES_sTd_10_rev | 5'-GCCTGCAGCGGCCGCTACTAGTACCCGCTTCAACAATTGCCAGAA | 10.08.2012 | ||
| I C8 | MS22 SB-prep-3P-1 | 5'-GCCGCTGCAGTCCGGCAAAAAA | 20.08.2012 | ||
| I C9 | MS23 SB-prep-2Ea | 5'-ATGAATTCCAGAAATCATCCTTAGCG | 20.08.2012 | ||
| I D1 | MS24 Suffix_std_10_fwd | 5'-ACTAGTAGCGGCCGCTGCAG | 20.08.2012 | ||
| I D2 | MS25 Prefix_std_10_rev | 5'-TCTAGATGCGGCCGCGAATTC | 20.08.2012 |
