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Team:NCTU Formosa
From 2012.igem.org
Abstract
Greenhouse Effect and the limitation of the fossil fuels have always been a concern for people on Earth. So our team, NCTU_Formosa, hopes to produce isobutanol as a biofuel by bacteria. This concept will be achieved by E.coli containing plasmids with a lactose promoter, 5 RBSs, 4 zinc fingers, and 4 proteinic genes. With the temperature regulating system, we successfully prevent toxic intermediate interfering isobutanol production. Ecofuel E.coline can provide more affordable and higher quantity of biofuel, furthermore lower the burden of our Earth.
News
25 Sep
In order to make sure our tolerance data, we used totally three days to do this experiment. Also we collect the concentrate of isobutal in our test experiment every 4 hours in a day.And we are continuing update our wiki and part wiki.
19 Sep
This week, we try to assemble our main parts together and incubate bacteria to test the productivity of isobutanol. Team work tops everything during our cooperation. So, to finish our wiki, each of our teammates are taking charge of different parts of wiki edition, and we took turn to collect tubes for 24 hours in the isobutanol tolerance test. Except for working in the wet lab, we also had fun times taking group photos and new films! By the way, a reporter wrote a special column for us about iGem & Synthesis Biology on Sep. 17. We're now processing the information. Please stay tune and check it out then!
14 Sep
For the experiment, there are new works in for everyone. We have to measure the O.D. on 42 degree and 30 degree, test all the different system we have. The most important is that we should find the G3 part which was made last year, insert into our recent part, and measure the yield. For the wiki, all the deadline of very page sould complete by 9/24. Last, we set up a new group for devising the poster.
