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From 2012.igem.org

SDS-PAGE

Pelleted cells were resuspended in 200 uL Milli-Q H2O.
Transferred 50 uL of suspension into new Eppendorf tubes and combined with 50 uL 2x TruSep sample buffer.
Using a Hamilton syringe, the cells were sheared.
Centrifuged the preparations for 3 mins @ 13,000 rpm.
Loaded 20 uL of the supernatant in to the gel.