Team:Macquarie Australia/Protocols/SDSPAGE



Pelleted cells were resuspended in 200 uL Milli-Q H2O.

Transferred 50 uL of suspension into new Eppendorf tubes and combined with 50 uL 2x TruSep sample buffer.

Using a Hamilton syringe, the cells were sheared.

Centrifuged the preparations for 3 mins @ 13,000 rpm.

Loaded 20 uL of the supernatant in to the gel.

Electrophoresis was conducted at constant voltage (200 V) for one hour.