SDS-PAGE

Pelleted cells were resuspended in 200 uL Milli-Q H2O. Transferred 50 uL of suspension into new Eppendorf tubes and combined with 50 uL 2x TruSep sample buffer. Using a Hamilton syringe, the cells were sheared. Centrifuged the preparations for 3 mins @ 13,000 rpm. Loaded 20 uL of the supernatant in to the gel.