Team:Wageningen UR/Journal/week21

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Project Overview

The Constructor

Human Practices

Last year's project: Synchronized Oscillatory System

Wageningen UR

Our Sponsors:

We thank our sponsors: AFSG, Agilent Technologies, Baseclear, DSM, ERASynBio, Gilson, Middelhoven Fund, NBV, SBN, SurfRender, VLAG, Wageningen UR

week 21: 17 September - 23 September

Hepatitis B inside modification

Colony PCR of Hepatitis B core protein behing an IPTG promoter in pSB1C3 backbone with sequencing primers

-> the gel shows fragments of the expected size (about 1kb)

GFP modification

17 September

ligation of the PCR products from 14 September (mixing the duplo samples together) with pJET and transformation with JM109

18 September

colony PCR of the transformation from 17 September

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