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From 2012.igem.org

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Overall project

We are currently pursuing the concept of brewing a gluten-free beer using a yeast that secretes an enzyme to break down the gluten. (1-2 paragraphs)

Project Details

-yeast

 assemble plasmids
 His tag plasmids
  n-terminus
  c-terminus
 clone mating factor-alpha

-bacteria

 assemble plasmids
 His tag plasmids
  n-terminus
  c-terminus
 secretion

-Prolyl Endoprotease-A.niger

 viable option?
 source?  cloning vs synthesize
   E.coli
   yeast

-functional protease assay

 fluorophore/quencher
 mass spec
 elisa

Results