Team:KIT-Kyoto/Notebook-week6

September 11th

The female-virgin flies (yw) and male flies (yw) were again collected and separately kept at 25℃.

September 12th

The female-virgin flies (yw) and male flies (yw) were again collected and separately kept at 25℃.

September 13th

The female-virgin flies (yw) and male flies (yw) were again collected and separately kept at 25℃.

September 14th

The adult male and virgin female flies from microinjected embryos were mated with yw virgin female flies and yw male flies, respectively. Mated flies were transferred to the new food vials in every three days to lay eggs as much as possible. The female-virgin flies (yw) and male flies (yw) were again collected and separately kept at 25℃.

September 15th

The female-virgin flies (yw) and male flies (yw) were again collected and separately kept at 25℃.

September 16th

The adult male and virgin female flies from microinjected embryos were mated with yw virgin female flies and yw male flies, respectively. In total 83 flies from microinjected embryos were mated with yw flies. Mated flies were transferred to the new food vials in every three days to lay eggs as much as possible.

September 17th

The progeny flies were inspected by dissecting microscope to look for the successfully transformed w+ red eye flies (red eye screening). However, no red eye fly was found.