Team:Shenzhen/Notebook/YAO.Channel

Context 1

• Apr. 18th - Apr.22th

Events:

We find some papers to read to know something about transport systems

- Problem to be solved:

- 1)kinds of transport systems in the mitochondria of yeast；

- 2)the way these systems transport proteins

- 3)Important proteins in the transport system

- Important papers we read:

- 1) How mitochondria import hydrophilic and hydrophobic proteins. Agnieszka Chacinska, Nikolaus Pfanner and Chris Meisinger.TRENDS in Cell Biology Vol.12 No.7 July 2002

- 2) Converting bacteria to organelles: evolution of mitochondrial protein Sorting. Johannes M. Herrmann. TRENDS in Microbiology Vol.11 No.2 February 2003

- 3) Multiple pathways for sorting mitochondrial precursor proteins. Natalia Bolender 1,2, Albert Sickmann3, Richard Wagner 4, Chris Meisinger 1 & Nikolaus Pfanner 1+... EMBO reports (2008) 9, 42–49. doi:10.1038/sj.embor.7401126

- 4) Versatility of he mitochondrial protein import machinery. Nikolaus Pfanner and Andreas Geissler. NATURE REVIEWS | MOLECULAR CELL BIOLOGY. VOLUME 2 | MAY 2001 | 339-349

- 5)Evolution of the Molecular Machines for Protein Import into Mitochondria , Pavel Dolezal et al. Science 313,314 (2006);

- Something we are still unclear:

- 1)Weather the present situations of the transport systems allow us to do a good classification

- 2)What’s the mechanism of assembling membrane proteins to the inner membrane.

- 3)If we use chloroplast’s SRP system, how to assemble the related proteins?

- 4)If we add new system in the outer membrane,how to let it work normally.

Contributors:

- Qiwu XU, unguang XU, Haojun JIANG, Jie WANG, Qiong WU, Senwei TANG, Qinsi LIANG

• Apr. 27th

Events:

- We read some papers and share findings with others team members in our seminar.

- Things we have known:

- 1)More detail about how do proteins transporting membrane.And we find some 3D pictures that could clearly show this process.

- 2)Proteins that play important roles in this process.

- 3)The ways that these proteins assembled to membrane.

- Our next step：

- Since we have acquainted entirely about the transport system about mitochondria.And our next step is to reform this system .

- Important papers we read:

- 1)Versatility of he mitochondrial protein import machinery. Nikolaus Pfanner and Andreas Geissler. NATURE REVIEWS | MOLECULAR CELL BIOLOGY. VOLUME 2 | MAY 2001 | 339-349

- 2)Functional cooperation and separation of translocators in protein import into mitochondria, the double-membrane bounded organelles. Toshiya Endo1,2,*, Hayashi Yamamoto1 and Masatoshi Esaki1, Journal of Cell Science 116, 3259-3267 © 2003 The Company of Biologists Ltd.doi:10.1242/jcs.00667

- 3)ignal-anchored Proteins Follow a Unique Insertion Pathway into the Outer Membrane of Mitochondria*，Uwe Ahting‡§, Thomas Waizenegger‡, Walter Neupert, and Doron Rapaport，JBC Papers in Press, October 22, 2004, DOI 10.1074/jbc.M410905200

- 4)Assembling the mitochondrial outer membrane, Nikolaus Pfanner,2 November 2004;doi:10.1038/nsmb852

Contributors:

- Denghui Liu, Luohao Xu, Chengran Zhou, Qinsi Liang.

• May. 6th
(请统一时间格式)

Events:

- We held a meeting to discuss how to reform the system .

- Our conclusion:

- We can use the TOM complex and TIM complex of chloroplast to replace these of mitochondria.But we baven’t acknowledge enough information of chloroplast,so we have to read more papers about transport system of chloroplase.

Contributors:

- Chengran Zhou,Luohao Xu,Denghui Liu,Yu Chen,Qinsi Liang, Kang KANG

• May. 7th - May .21th

Events:

- Read important papers:

- 1)Svesnikova, N., Grimm, R., Soll, J. & Schleiff, E. Topologystudies of the chloroplast protein import channel Toc75.Biol. Chem. 381, 687–693 (2000).

- 2)Functional cooperation and separation of translocators in protein import into mitochondria, the double-membrane bounded organelles. oshiya Endo1,2,*, Hayashi Yamamoto1 and Masatoshi Esaki1, Journal of Cell Science 116, 3259-3267 © 2003 The Company of Biologists Ltd.doi:10.1242/jcs.00667

Contributors:

- Denghui Liu, Luohao Xu, Chengran Zhou, Qinsi Liang.

• May. 31th

Events:

- Seminar about how to reform transport system of mitochondria.

- Important papers:

- 1)Evolution of the Molecular Machines for Protein Import into Mitochondria , Pavel Dolezal et al. Science 313,314 (2006);

- 2)Converting bacteria to organelles: evolution of mitochondrial protein Sorting. Johannes M. Herrmann. TRENDS in Microbiology Vol.11 No.2 February 2003

- 3)Signal-anchored Proteins Follow a Unique Insertion Pathway into the Outer Membrane of Mitochondria*，Uwe Ahting‡§, Thomas Waizenegger‡, Walter Neupert, and Doron Rapaport¶，JBC Papers in Press, October 22, 2004, DOI 10.1074/jbc.M410905200

Contributors:

- Kang Kang, Yu chen, Denghui Liu, Luohao Xu, Chengran Zhou, Qinsi Liang.

• Jun. 11th

Events:

- Seminar about our experiment.

- We had a discussion about how to design our experiment.

Contributors:

- Qiwu Xu, Kang Kang, Denghui Liu, Luohao Xu, Chengran Zhou, Qinsi Liang.

• Jun. 15th - Jul. 20

Events:

- Prepare for our experiment and read more papers about transport system.

Contributors:

- Qiwu Xu,Chengran Zhou

• Jul. 20th - Aug. 20

Events:

- Experiment with construction of signal peptide BioBricks.

Contributors:

- Qiwu Xu, Mingyu Gan.

• Aug. 20th - Sep. 15th

Events:

- YAO. Channel Biobricks,story, pictures preparation and presentation design.

Contributors:

- Yu CHEN, Kang KANG, Denghui Liu, Luohao Xu, Chengran Zhou, Qinsi Liang.Mingyu Gan, Haosen Chen.

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