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From 2012.igem.org

Communication Bacteria<->Xenopus

Overview

Before engineering the plant hormonal system directly by the tadpole, we tested first if this hormone was inoffensive for the tadpole. We thought about how to deliver this hormone directly into the tadpoles and the embryoos. The first, and most obvious, decision was to inject directly into the embryoos with µinjection, and, for the tadpoles to mix the MMR medium with auxin. The second, and most creative, was to use bacteria as a delivery machine in order to create a communication between two engineered organisms.

Steps