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Team:TU Darmstadt/Materials/Materials
From 2012.igem.org
Contents |
Chemicals
CARL ROTH GMBH + CO. KG
- acetic acid
- acrylamide (rotiphorese)
- agar-agar kobe I
- ammonium persulfate (APS)
- ammonium sulfate
- ampicillin
- ß-mercaptoethanol
- chloramphenicol
- coomassie brillant blue 250
- D-(+)-glucose
- ethanol
- ethylenediaminetetraacetic acid (EDTA)
- gloves
- glycerol
- HCl
- imidazole
- isopropyl alcohol
- kanmycin
- KCl
- KH2PO4
- L-(+)-arabinose
- Na2HPO4
- NaCl
- NaOH
- NiCl2
- sodiumdodecylsulfate (SDS)
- tributyrine
- tricine
- TRIS-HCl
- tryptone
- yeast extract
AppliChem GmbH
- bromthymol blue)
- tetramethylethylenediamine (TEMED
Sigma-Aldrich Co. LLC.
- bromphenol blue
- hydrocinnemonium chloride
- para-nitrophenol
- succinyl chloride
- tween 20
New England Biolabs GmbH
Enzymes
- EcoRI
- EcoRI HF
- NcoI HF
- NdeI HF
- NotI HF
- Phusion
- PstI HF
- Q5 Polymerase Hotstart
- Q5 Polymerase normal
- T4 DNA Ligase
- XbaI HF
Buffer
- 2-log DNA marker
- bovine serum albumin (BSA 100x)
- Buffer 4
- dimethylsulfoxide (DMSO)
- DNA loading Dye 6x
- GC Phusion 5x Buffer
- GC Q5 Enhancer Buffer
- MgCl2
- Phusion HF Buffer 5x
- Q5 reaction Buffer
- T4 Ligase Buffer 10x
Promega GmbH
Enzymes
- EcoRI
- PstI
- SpeI GQ
- T4 Ligase
- TEV Protease
- XbaI GQ
Buffer
- Buffer B
- Buffer D
- Buffer H
- Go Tag Puffer 5x
- Go Tag Puffer 5x prestained
- Multi Core 10x Buffer
- T4 Ligase Buffer 10x
Kits
- Pure Yield Plasmid Miniprep System
- Wizard SV Gel and PCR Clean Up System
