Overview of used components

Plasmids

Supplied by the iGEM distribution kit 2012
pSB1C3
J63009
pSB4C3
pSB3C5
pSB1AT3
(GFP plasmid)

Vectors

Supplied by the iGEM distribution kit 2012
J45320
(GFP?)
Ordered M.ScaI synthesized vector

Chemicals

IPTG
EtBr
Chloramphenicol
Ampicilin
Kanamycin

Substances

AGAR
LB

Bacteria

DH5-alpha

Kits

Qiagen mini-prep
Qiagen Gel extraction
Qiagen PCR purification

Methods

Gibson Assembly

Uva/VU and RIVM safety (GMO’s)