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From 2012.igem.org

week 14: 30 july - 5 august

Lab work

Bricking Hepatitis B

• Miniprepping of successful transformants from the transformation (Hep B + BBa_J04500)
• Digestion check of those minipreps

-> this digestion check was repeated 2 times (once with a longer incubation time and once with a higher amount of DNA in the digestion mixture) until the insert size could be seen on the gel

the digestion check confirmed the right insert sizes in the samples they where expected (slots 3,4,10,11)

TuYV

• The four 'brickable' TuYV CP gene amplicons were digested, ligated into the backbone from BBa_J04450 and transformed into our electrocompetent Mach1 E coli. Despite negative results on two PCR checks, minipreps were done anyway and a third PCR check showed presence of all of our inserts in their respective minipreps!

PLRV

The PCR products obtained after Reverse Transcription followed by PCR were used as template to add iGEM prefix and suffix.

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