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Team:British Columbia/Notebook
From 2012.igem.org
Contents |
June 5th
Grew cell cultures of EPI 300, BL21, and DH5α, with plasmid PIJ 790 in them, harvested, and stored at -80 freezer.
-Ruichen
June 6th
Learned how to electrophorese cells with biobricks (1-7B, 2-17F, 2-14N, 2-9B, 3-13M, 3-14E, 4-3I, 5-1A, 5-12O), and plated them on plates to grow colonies.
Tested KAN plates with control.
-Ruichen
June 7th
Retreated the plates previously spread, KAN plates worked and most of them grew into colonies or covered plates completely. (advised to spread the plates with 50µl recovered cells instead 100µl in the future for better colony identification).
Made more antibiotics of Amp (10µg/ml) and Chlor (34µg/ml), and more agar plates of (Amp and Chlor) (half bagful respectively).
Electrophoresed 1-1N, 2-2K, 1-5P, 2-21O using DH5α, and plated them.
-Ruichen
In the afternoon, we (Ruichen, Mehul, Grace) made many more K12 competent cells, which we put into storage. (The protocol is now on the wiki.) There was also talk from Jacob and John about buying $0.50 worth of gasoline/diesel from the nearest gas station to test solvent resistance of some of our cells. I left before that happened, though. (Would have been fun to see the attendant's reaction!)
- grace
June 8th
Tried to make more EPI 300 cells, though no obvious growth even after hours for the second time, and decided to leave it over night to see if there are any changes.
Electrophoresed K12 with PIJ 790 plasmid, and plated them on Chlor plates with control plates to examine the plates.
- Ruichen
