Ligation

pCEP4 and the LovTAP readout were ligated together. Chloramphenicol plates were prepared for the culture of Biobricks and their empty vector psB1C3 (the antibiotic was at 34 µg/ml, we kept that concentration). Ran a gel of the pGL-SEAP control PCR with both buffers, found out that only one colony (the 11th one) had worked. Took the pHY42 plates out (they were transformed the evening before). Put them at 4°C, to be used for a later maxiprep culture.