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From 2012.igem.org

week 14: 30 july - 5 august

Lab work

Bricking Hepatitis B

Monday:

• Miniprepping

of 8 colonies from the transformation (Hep B + BBa_J04500)

• Digestion check

of those minipreps

Wednesday:

• Second digestion check of the minipreps (with higher amount of DNA)

the digestion check confirmed the right insert sizes in the samples they where expected (slots 3,4,10,11)

TuYV

• The four 'brickable' TuYV CP gene amplicons were digested, ligated into the backbone from BBa_J04450 and transformed into our electrocompetent Mach1 E coli. Despite negative results on two PCR checks, minipreps were done anyway and a third PCR check showed presence of all of our inserts in their respective minipreps!

PLRV

The PCR products obtained after Reverse Transcription followed by PCR we used as template to add iGEM prefix and suffix.

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