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Team:UNITN-Trento/Parts
From 2012.igem.org
Our Parts
Here you can find our favourite Parts and all the Parts we built. Be also sure to check out our Team Parts Page on the Registry website!
FAVORITES
K731030 - Inducible araC-pBAD promoter regulating M256I CysE
The CysE M256I gene (K731010) is here regulated by the araC-pBAD promoter (K731201), which is inducible by arabinose. This part was sub cloned and characterized in psB3C5.
Registry PageK731400 - IPTG inducible Cysteine desulfhydrase (CysDes)
This part is composed of K731300 and K731600. It is an IPTG inducible device regulating the expression of a Cysteine Desulfhydrase from Treponema Denticola (CysDes). This enzyme is an aminotransferase that converts cysteine into pyruvate, ammonia and hydrogen sulfide. The part has been successfully operated and characterized in psB1C3 and psB4K5.
Registry PageK731710 - Platform for terminators analysis with E. coli RNA polymerase by fluorimetric assay
This part is used to analyse terminators: two subsequent fluorescent proteins (mCherry and A206K Venus), separated by Prefix-Suffix linker, allow standardization of terminator's presence effect. The vector was obained by mutagenesis of BBa_K731700, replacing T7 promoter with pTAC. It contains Ampicillin resistance gene and is inducible by IPTG (pTAC-lacO). The combined use of BBa_K731710 and BBa_K731700 allow also to analyse any potential difference in terminators' activity due to different polymerases.
Registry Page
