Team:TU Darmstadt/Materials/Bromphenol blue

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Contents

Bromphenol blue

About

Bromphenol blue is a size marker used in agarose gel electrophoresis[1] that does also work as a pH indicator. Below pH=3.0 it is yellow and blue-purple at more basic pH. Its specific band depends on the gel concentration:[2]

 % Agarose bp
0,7 1000
1 500
2 150
3 50

Application

References

[1] http://en.wikipedia.org/wiki/Bromophenol_blue

[2] http://openwetware.org/wiki/Bromophenol_blue

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