Team:TU Darmstadt/Materials/SpeI

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SpeI

About

SpeI is a endonucleasic restriction enzyme isolated from Sphaerotilus natans. It creates stick ends with 5' end overhang.

Working conditions

The recognition motive of SpeI is:[1] 

5' ACTAGT --> 5'---A     CTAGT---3'
3' TGATCA --> 3'---TGATC     A---5'

Buffer: NEBuffer 2 + BSA

SpeI requires at least 2bp overhang on its cutting site (100% cleavage efficiency).[2]

Supplier

  • NEB http://www.neb.com/nebecomm/products/productR3133.asp

References

[1] http://en.wikipedia.org/wiki/List_of_restriction_enzyme_cutting_sites:_S#Whole_list_navigation

[2] http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/cleavage_linearized_vector.asp

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