Team:TU Darmstadt/Materials/EcoRI

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EcoRI

About

EcoRI is a endonucleasic restriction enzyme isolated from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli RY13]. It creates stick ends with 5' end overhang.

Working conditions

The recognition motive of EcoRI is:[1] 

5' GAATTC --> 5'---G     AATTC---3'
3' CTTAAG --> 3'---CTTAA     G---5'

EcoRI can exhibit star activity depending on the conditions present in the reaction and needs at least 1bp overhang on its cutting site.[2]

Supplier

  • NEB http://www.neb.com/nebecomm/products/productR3101.asp

References

[1] http://en.wikipedia.org/wiki/EcoRI [2] http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/cleavage_linearized_vector.asp

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