Team:TU Darmstadt/Materials/Phusion

From 2012.igem.org

Revision as of 13:14, 11 September 2012 by Adrian E (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Contents

Phusion

About

Phusion is a Pyrococcus Pfu-like DNA polymerase with a dobule-strand DNA binding domain, resulting in an enhanced processivity and higher activity than other available DNA polymerases like taq and creates blunt ends.

Working conditions

  • 98°C denaturation temperature
  • ~30 s/kb extension velocity (at 72°C)
  • (TM-3)°C=TA

References

  • http://www.neb.com/nebecomm/ManualFiles/manualF-540.pdf
Retrieved from "http://2012.igem.org/Team:TU_Darmstadt/Materials/Phusion"