Transition from a prokaryotic bacteria to yeast.

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Currently I'm looking into 3 aspects of the transition to yeast:

1. Subcloning into a yeast expression plasmid.

2. Codon bias.

Lots of details on the codon bias in yeast: [http://www.jbc.org/content/257/6/3026.full.pdf Yeast Codon bias].

3. Secreting enzyme into beer.

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