User contributions

From 2012.igem.org

For Ajl58 (talk | block log | logs)

(Latest | Earliest) View (newer 50 | older 50) (20 | 50 | 100 | 250 | 500)

00:18, 4 October 2012 (diff | hist) Team:Yale/Notebook ‎ (→Andriana Lebid)
00:16, 4 October 2012 (diff | hist) Team:Yale/Notebook ‎ (→Andriana Lebid)
00:14, 4 October 2012 (diff | hist) Team:Yale/Notebook ‎ (→Andriana Lebid)
00:06, 4 October 2012 (diff | hist) Team:Yale/Notebook ‎ (→Jae Seong No)
00:06, 4 October 2012 (diff | hist) N User:Jn323/7 August 2012 ‎ (Created page with "====Electroporation==== *Three strains used: BS168, BS1A833, BSPY79 *Filter-sterilize buffers =====Preparation of Cells===== *Check O/N growth :* Back-Dilution prepared until OD6...") (top)
00:05, 4 October 2012 (diff | hist) N User:Jn323/2 August 2012 ‎ (Created page with "====Kanomycin Plate Preparation==== *Autoclave two 500mL LB-Agar solutions *Add 0.5mL of stock kanomycin (50mg/mL) in one 500mL LB-Agar solution (final conc. 50ug/mL) *Add 0.2mL ...") (top)
00:05, 4 October 2012 (diff | hist) N User:Jn323/1 August 2012 ‎ (Created page with "====Electrophoresis==== =====Preparation of Cells===== *Check O/N growth :* OD600 reading - 1.8 :* Back-Dilution prepared until OD600 reading was between 0.4 and 0.8 '''Following...") (top)
00:05, 4 October 2012 (diff | hist) N User:Jn323/31 July 2012 ‎ (Created page with "====Preparation for Electroporation==== *Check overnight growth :*All grew (both plates and liquid cultures) except BS PY79 :*Error made in growth - BS PY79 NOT resistant to Spc;...") (top)
00:04, 4 October 2012 (diff | hist) N User:Jn323/30 July 2012 ‎ (Created page with "====Preparation for Electroporation==== *Inoculate cells into 10mL of LB media (scrape glycerol stock with metal loop and place into LB solution in culture tube) :*ADP1, EcNR2, B...") (top)
00:04, 4 October 2012 (diff | hist) N User:Jn323/24 July 2012 ‎ (Created page with "====PCR - Amplification of Kan cassette and RiAFP cassette==== ====Gel Verification & Imaging==== ====Gel Extraction====") (top)
00:04, 4 October 2012 (diff | hist) N User:Jn323/23 July 2012 ‎ (Created page with "====PCR - Amplification of Kan cassette and RiAFP cassette==== ====Gel Verification & Imaging====") (top)
00:04, 4 October 2012 (diff | hist) N User:Jn323/20 July 2012 ‎ (Created page with "====Suspension of Primers==== ====Primer Dilution Stock====") (top)
00:03, 4 October 2012 (diff | hist) N User:Jn323/18 July 2012 ‎ (Created page with "====Primer Confirmation / Resuspension====") (top)
00:03, 4 October 2012 (diff | hist) N User:Jn323/17 July 2012 ‎ (Created page with "====Preparation of EcNR2====") (top)
00:03, 4 October 2012 (diff | hist) N User:Jn323/16 July 2012 ‎ (Created page with "====Changes to Primers====") (top)
00:03, 4 October 2012 (diff | hist) N User:Jn323/11 July 2012 ‎ (Created page with "====Primer Designing====") (top)
00:02, 4 October 2012 (diff | hist) N User:Jn323/10 July 2012 ‎ (Created page with "====Reconstruction of Plasmid pSB1AK8-eGFP-TEV-RiAFP====") (top)
00:02, 4 October 2012 (diff | hist) N User:Jn323/9 July 2012 ‎ (Created page with "====Preparation of LB Agar Plates====") (top)
00:02, 4 October 2012 (diff | hist) N User:Jn323/6 July 2012 ‎ (Created page with "====Origami Cell Antibiotic Test====") (top)
00:01, 4 October 2012 (diff | hist) N User:Jn323/5 July 2012 ‎ (Created page with "====Serial Dilutions==== *Grow overnight culture of Normal Origami cells, Normal Origami cells exposed to IPTG, Transformed Origami cells exposed to IPTG :*Grown in ~5mL LB media...") (top)
00:01, 4 October 2012 (diff | hist) N User:Jn323/2 July 2012 ‎ (Created page with "====PCR (Site-Directed Mutagenesis)====") (top)
00:01, 4 October 2012 (diff | hist) N User:Jn323/26 June 2012 ‎ (Created page with "====Freeze-Thaw Assay, 1-hour cycles==== =====IPTG Induction===== *Scrape off 4-5 colonies of transformed origami cells and untransformed origami cells from respective plates cul...") (top)
00:00, 4 October 2012 (diff | hist) N User:Jn323/25 June 2012 ‎ (Created page with "====Cell Transformation==== *Thaw Origami cells on ice *Aliquot 50uL of competent cells into two pre-chilled 15mL culture tubes *Add DNA (1-5ng in 1-5uL) to one of the culture tu...") (top)
00:00, 4 October 2012 (diff | hist) N User:Jn323/22 June 2012 ‎ (Created page with "====Freeze-Thaw Assay #1, 24-hour cycle==== =====IPTG Induction===== *Scrape off 4-5 colonies of transformed origami cells and untransformed origami cells from respective plates ...") (top)
23:59, 3 October 2012 (diff | hist) Team:Yale/Notebook ‎ (→Jae Seong No)
23:57, 3 October 2012 (diff | hist) N User:Andriana/21 June 2012 ‎ (Created page with "====I. Started culture with the only plate showing bacterial growth – mutants 15+16====") (top)
23:57, 3 October 2012 (diff | hist) N User:Andriana/20 June 2012 ‎ (Created page with "====I. Finished the presentation powerpoint==== ====II. Continued freeze-thaw selection pressure tests for MAGE including plating of solution samples==== ====III. Measured approx...") (top)
23:56, 3 October 2012 (diff | hist) N User:Andriana/19 June 2012 ‎ (Created page with "====I. Transformed DH5-α cells using PCR reactions 7+8, 9+10, 15+16, and 29+30==== ====II. Plated the four transformed samples==== ====III. Continued record-keeping and worked o...") (top)
23:56, 3 October 2012 (diff | hist) User:Andriana/18 June 2012 ‎ (top)
23:55, 3 October 2012 (diff | hist) N User:Andriana/17 June 2012 ‎ (Created page with "====I. Finished concentration and dilution calculations==== ====II. Made all diluted (25 mM) primer solutions==== ====III. Ran a 7-8 mutation PCR, but forgot to set melting tempe...") (top)
23:55, 3 October 2012 (diff | hist) N User:Andriana/18 June 2012 ‎ (Created page with "====I. Finished concentration and dilution calculations==== ====II. Made all diluted (25 mM) primer solutions==== ====III. Ran a 7-8 mutation PCR, but forgot to set melting tempe...")
23:54, 3 October 2012 (diff | hist) N User:Andriana/16 June 2012 ‎ (Created page with "====I. Green Experiment Results:==== 12 hours after plating – about equal, abundant colony growth all across except for treatment D (no colonies): [[File:Andriana_Notebook_7...") (top)
23:54, 3 October 2012 (diff | hist) N User:Andriana/15 June 2012 ‎ (Created page with "====I. Meeting with Kaury Kucera about primer designs:==== * Confirmed findings about primer design for site-directed mutagenesis as follows: :# Mutations should be made closer...") (top)
23:53, 3 October 2012 (diff | hist) N User:Andriana/14 June 2012 ‎ (Created page with "====I. Assay Continued==== <ol start="7"> <li>After being frozen for 24 hours, BL 21 cell samples (normal and transformed – normal on plain agar plates and transformed on amp...") (top)
23:53, 3 October 2012 (diff | hist) N User:Andriana/13 June 2012 ‎ (Created page with "====I. 24 Hour Assay Continued==== <ol start="3"> <li>Centrifuged out pellets of all 4 cell samples grown overnight (all reached the stationary phase due to significant murkines...") (top)
23:53, 3 October 2012 (diff | hist) N User:Andriana/12 June 2012 ‎ (Created page with "*Ampicillin and kanamycin plates with transformed BL21 and origami cells had bacterial growth, but control p...") (top)
23:52, 3 October 2012 (diff | hist) N User:Andriana/11 June 2012 ‎ (Created page with "====I. Continued designing primers==== ====II. Rechecked primers==== ====III. More autoclaving of glassware==== ====IV. Miniprepped DH5-α cells grown from our transformation====...") (top)
23:52, 3 October 2012 (diff | hist) N User:Andriana/10 June 2012 ‎ (Created page with "====I. Started growing a batch of newly transformed DH5-α cells==== ====II. Continued designing primers==== ") (top)
23:52, 3 October 2012 (diff | hist) N User:Andriana/9 June 2012 ‎ (Created page with "====I. Continued designing primers==== ====II. Miniprepped the rest of RiAFP+GFP DH5-α cells==== ====III. Ordered some primers==== ") (top)
23:51, 3 October 2012 (diff | hist) N User:Jn323/8 June 2012 ‎ (Created page with "====I. Began growing stock RiAFP+GFP DH5-α cells (dissolve some scraped cells in 10 mL LB medium with 10 microliters of both ampicillin and <s...") (top)
23:51, 3 October 2012 (diff | hist) N User:Andriana/7 June 2012 ‎ (Created page with "====I. Continued transformation of BL21 cells==== ====II. Continued and finished Transformation of DH5-α cells:==== ''NOTE: Conduct all procedures aseptically, sterilizing the...") (top)
23:51, 3 October 2012 (diff | hist) N User:Andriana/6 June 2012 ‎ (Created page with "====I. Began growing DH5-α cells in a medium==== # Dilute one colony scraped from an agar plate in 5 mL of LB in a 25 or 50 mL flask and incubate overnight in a shaker at 37C (...") (top)
23:50, 3 October 2012 (diff | hist) N User:Andriana/5 June 2012 ‎ (Created page with "====I. Plated DH5-α cells on plain agar plate (making competent DH5-α cells)==== ''Note: Instead of plating, growth in a culture flask can be begun directly'' # Have a plain a...") (top)
23:50, 3 October 2012 (diff | hist) N User:Andriana/4 June 2012 ‎ (Created page with "====I. Made necessary purchases==== ====II. Made antibiotic solutions (use sterile equipment):==== =====Ampicillin Stock:===== # <span style...") (top)
23:49, 3 October 2012 (diff | hist) N User:DrJones1935/2 June 2012 ‎ (Created page with "====I. Unpacked==== ====II. Made plain agar plates:==== :Protocol: ::# Agar– 15 g ::# LB broth – 25g (10 g Tryptone, 5 g Yeast Extract, 10 g NaCl) ::# DI water – 1L ::# C...") (top)
23:49, 3 October 2012 (diff | hist) N User:Andriana/1 June 2012 ‎ (Created page with "====I. Unpacked==== ") (top)
23:49, 3 October 2012 (diff | hist) Team:Yale/Notebook ‎ (→Andriana Lebid)
23:43, 3 October 2012 (diff | hist) Team:Yale/Notebook ‎ (→David Lim)
23:41, 3 October 2012 (diff | hist) Team:Yale/Notebook ‎ (→David Lim)
23:40, 3 October 2012 (diff | hist) N User:DrJones1935/2 October 2012 ‎ (Created page with "====I. Check Overnight Liquid Cultures==== =====RESULTS:===== {| class="wikitable" |- ! Sample !! Kan50 !! Kan + Cm37 !! Kan + Tet15 !! IPTG (0.5 mM) + Kan !! IPTG + Kan + Cm !...") (top)

(Latest | Earliest) View (newer 50 | older 50) (20 | 50 | 100 | 250 | 500)