User:Andriana/12 June 2012

From 2012.igem.org

  • Ampicillin and kanamycin plates with transformed BL21 and origami cells had bacterial growth, but control plates were clear:
File:Andriana Notebook 1.jpg
Analysis: origami cells are shown to be more efficient at plasmid take-up

Contents

I. Worked on primer designs

II. Made a few practice DNA gels

III. Attended a meeting/presentation relating to MAGE

IV. Picked up a centrifuge

V. Prepared cell-broth solutions to be grown overnight for the E. coli frozen at -80C for 24 hours assay:

  1. diluted 500 microliters of leftover control BL21 cells (refrigerated at 4C from Monday) with 4.5 mL of LB broth (no antibiotics) in a 15 mL polypropylene test tube and left overnight in a 37C shaker.
  2. Inoculate 2 colonies of BL21 transformed cells in 5 mL LB broth (with 5 microliters of ampicillin and 5 microliters of kanamycin) in a 50 mL flask and leave to incubate overnight in a 37C shaker. The same for transformed origami cells.

NOTE about colony growth: Origami cells appear to be more competent as the plate with origami cells contained many more colonies than that with transformed BL21’s: (100 vs. 30)

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