Team:Osaka/week5

August 26 (Sun)

1. Colony check
   • 1-1G,1-5A → Successfully transformed
   • 5-23O,1-7A → Transformed cells didn't grow on plates
2. Transfer to liquid culture:1-1G,1-5A
3. Preparation of LB agar plates (Tet)

August 27 (Mon)

1. miniprep(1-1G,1-5A)
2. Transformaton of ligation products into DH5α E.coli:155,156
   • Transformed cells did not grow on plates

August 28 (Tue)

1. Preparation of LB agar plates (Amp,Cam)
2. Ligation(155,156)
3. Transformaton of ligation products into DH5α E.coli:155,156,152(positive control)
4. To liquid culture(152,155,Rosetta wild type)

August 29 (Wed)

1. miniprep(155①~④)
2. Restriction digests(155,1-1G)
3. Electrophoresis(153,1-1G)
4. Transformation(156)
5. To liquid culture(152,155,WT)
   • 155 → turned red

August 30 (Thu)

August 31 (Fri)

September 1 (Sat)

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