Team:TU-Eindhoven/LEC

• Introduction
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    information
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• Project
  • Project description
  • Lab approach
  • Device information
  • Initial model
  • Yeast model
  • Lab results
  • Our BioBricks^TM
  • Our
    accomplishments
• Considerations
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• Future applications
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    developments
• Notebook
  • Before week 1
  • Week 1
  • Week 2
  • Week 3
  • Week 4
  • Week 5
  • Week 6
  • Week 7
  • Week 8
  • Week 9
  • Week 10
  • Week 11
  • Week 12
  • After week 12
  • Lab protocols
• Partners
  • ICMS TU/e
  • Team collaborations
  • Attributions and Contributions
  • Our sponsors

The aim of this project of the iGEM team of Eindhoven University of Technology in 2012 is to design and produce a new multi-colored display in which genetically engineered cells function as pixels, analogous to how a at panel display works. The challenges are in reaching a high refresh rate of the screen, but also in creating a multi-colored prototype. There are three components to the display: genetically engineered cells that emit fluorescent light in response to an electrical stimulus (the “pixels”), a device to provide stimuli to each pixel (the “control grid”), and a computational model that predicts how cells will react to the stimuli from the device. The basic biological parts used in this project are yeast cells, fluorescent calcium sensors and calcium channels. The laboratory work will focus on integrating these parts and establishing the desired reactions to electrical stimuli in order to create the “pixels” of the display. Computational modeling will provide insight into the electrophysiology of yeast cells, the role of calcium channels and the most sensitive parameters in calcium homeostasis in order to optimize the biological system. Engineers will build an electronic device that is compatible with biological material to provide control over the pixels. The custom build hardware of the device is complemented with software written in LabView to generate images on the screen.