Team:EPF-Lausanne/Notebook/24 August 2012

From 2012.igem.org

Revision as of 14:42, 24 August 2012 by Diego.marcos (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)



Ligation of purified PCR products into backbones

PCR products have been purified the day before.

For Fussenegger:

  • TNFR into pGL
  • eGFP into pGL

SEAP can't be ligated yet! Digestion of pGL with MfeI required!

For LovTAP:

  • Matt's PCR LovTAP into pMP
  • RO into pcDNA3.1+


Protocol: None

Forgot to insert protocol.


Comments

Insert comments about what happened.