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From 2012.igem.org

SDS-polyacrylamide and native gel electrophoresis

Reagents & Materials

Procedure

• Wear gloves
• Clean glass slides with MQ and dry

1. Prepare running gel mix in a small beaker for 2 gels:
• 4 ml MQ
• 3.3 ml 30% acrylamide mix
• 2.5 ml 1.5 M TRIS pH 8.8
• 0.1 ml 10% SDS
• 0.1 ml 10% ammonium persulphate
2. Places glass slides in holders and tighten in rack.

polymerization:

3. Add 4 ul TEMED to the running gel mix, mix and pipet the gel into the slipes until the green edge.
4. Add a layer of iso-propanol on top of the polymerizing gel to equalize.