July 2 - July 8
Monday
Competence cells batch: 2.34 x 10^8 cfu/μg.
Tuesday
Gibson assembly with new cells. No colonies again.
Thursday
Gibson assembly with extra T5 gave no positives. KanR amplified finally (it was the inverted version KanRi).
Sunday</p> <p> PCR for C1 with KanRi. </p>