"

From 2012.igem.org

July 2 - July 8

Monday

• Competence cells batch: 2.34 x 10^8 cfu/μg.

Tuesday

• Gibson assembly with new cells. No colonies again.

Thursday

• Gibson assembly with extra T5 gave no positives.
• KanR amplified finally (it was the inverted version KanRi).

Sunday

• PCR for C1 with KanRi.