From 2012.igem.org
Weeks:
June |
July |
August |
September |
October |
November |
Week 9: 6th August - 12th August
Monday, 6th August
Reporter test
We had a lot of reporters BB but we didn't know if they're all working. We test them with a electrophoresis:
Two RFP, a GFP, a CFP and a YFP worked.
GFP BB
Digestion of GFP BB with EcoRI and PstI, gel extraction and DNA purification.
Goal: insertion in pCS2+ BB
Issue: Not enough DNA for ligation.
Tuesday, 7th August