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Team:Evry/Freezer
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Revision as of 14:35, 6 August 2012 by Chr.karine (Talk | contribs)
Freezer
Primers
| Code | Name | Sequence | Length | Tm | %GC | Comments | Date |
| P1 | Tir1-BB-FW | cgcttctagATGACGTACTTCCCGGAGG | 19 | 57 | 58 | 13th july | |
| P2 | Tir1-BB-Rv | ctactagtaTTATTATAGGATTTTAACAAAATTTGGTGC | 24 | 52 | 29 | 13th july | |
| P3 | SdMTir1-BB-Fw | AACTGAACTCACTACTGTACTTCTGTCACCAAATGACTAATGCTGCTCTAGTTACTGTCGCCAAG | 65 | 72 | 43 | 13th july | |
| P4 | SdMTir1-BB-Rv | TTGGACAGCCCAAGGATACTGCAACAAGTCCCTCCTCTGTCACAGCAGAATAACCAGCTACG | 62 | 76 | 52 | 13th july | |
| P5 | Tir1 SdM v02-Fw | tgcTgtgacagaggagggac | 20 | 60 | 60 | 25th july | |
| P6 | Tir1 SdM v02-Fw | ctagAgcagcattagtcatttgg | 23 | 55 | 43 | 25th july | |
| P7 | pCS2+BB-Fw | cgctgcagGAACTATAGTGAGTCGTATTACG | 23 | 51 | 39 | 13th july | |
| P8 | pCS2+BB-Rv | tgcatGAATTCGAATCGATGGGATCC | 21 | 54 | 48 | 13th july | |
| P9 | GFP-AID-BB-Fw | gaattcgcggccgcttctagagATGGCCACAACCATGGTG | 40 | 74 | 58 | 20th july | |
| P10 | GFP-AID-BB-Rv | tactagtagcggccgctgcagTTATTAAACCTTACGTTTCTTTTTAGGGAC | 51 | 70 | 43 | 20th july | |
| P11 | p2 Hsp70-Fw | ACTGTCGACCCCGTTTAGCAGGAAATAGCC | 30 | 67 | 53 | Heat Shock | 20th july |
| P12 | p2 Hsp70-Rv | ACTAAGCTTATTTGCGCTCCTTACAGTTTGC | 31 | 63 | 42 | Heat Shock | 20th july |
| P13 | p2 Flk-1-Fw | ACTGTCGACCTGGACTCTGGGCTAGGG | 27 | 68 | 63 | Vasculature | 20th july |
| P14 | p2 Flk-1-Rv | TCAAAGCTTGTTGACGTTTAGTCCAGGACAG | 31 | 64 | 45 | Vasculature | 20th july |
| P15 | p2 ROSA-Fw | ACTGTCGACTAGATGAAGGAGAGCC | 25 | 61 | 52 | Ubiquitous | 20th july |
| P16 | p2 ROSA-Rv | GATAAGCTTGGATCCCCGCAAACGCAC | 27 | 66 | 56 | Ubiquitous | 20th july |
| P17 | p2 CarA-Fw | ACTGTCGACGTCCCATACCAGTACAATGCAAT | 32 | 66 | 47 | muscles | 20th july |
| P18 | p2 CarA-Rv | AGTAAGCTTGTGCTGTGATTGAATTGGCTG | 30 | 63 | 43 | muscles | 20th july |
| P19 | IaaH-BB-Fw | gaattcgcggccgcttctagATGCGCGAAATGATTACGC | 19 | 55 | 47 | 23th july | |
| P20 | IaaH-BB-Rv | ctgcagcggccgctactagtaTTATTAGCCTTTTAACACTTTTTCG | 25 | 52 | 28 | 23th july | |
| P21 | IaaM-BB-Fw | gaattcgcggccgcttctagatgtttggaccggatttccc | 20 | 56 | 50 | 23th july | |
| P22 | IaaM-BB-Rv | ctgcagcggccgctactagtattattagtcccccagcgc | 39 | 55/73 | 56/59 | 23th july | |
| P23 | AID-Fw | cgcttctagGGAGCTGGTGCAGGCGCT | 18 | 64 | 72 | 25th july | |
| P24 | Sp6 | ATTTAGGTGACACTATA | 17 | 41 | 27 | for BB detection when sequenced | NP |
| P25 | T3 | AATTAACCCTCACTAAAGGG | 20 | 50 | 40 | for BB detection when sequenced | NP |
| P26 | primer_sequencing_pCS2+_Fw | CAAGTGTAGCGGTCACGCTG | 20 | 59 | 60 | 13th july | |
| P27 | primer_sequencing_pCS2+_Rv | GCGTAATCATGGTCATAGCTG | 21 | 52 | 48 | 13th july | |
| P28 | GFP_AID_BB_Fw_v2 | TGCATgaattcgcggccgcttctagagATGGCCACAACCATGGTG | 45 | 75 | 56 | ||
| P29 | IaaH_BB_Fw_v2 | TGCATgaattcgcggccgcttctagATGCGCGAAATGATTACGC | 44 | 74 | 52 | ||
| P30 | IaaM_BB_Fw_v2 | TGCATgaattcgcggccgcttctagatgtttggaccggatttccc | 45 | 74 | 53 | ||
| P31 | IRES_BB_F | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCGATCATTCAagagaTCCCC | 50 | 53/74 | 50/52 | ||
| P32 | IRES_BB_R | GTTTCTTCCTGCAGCGGCCGCTACTAGTAttgtggccatattatcatcg | 49 | 51/72 | 40/49 | ||
| P33 | BBa_G1005 | gtttcttcctgcagcggccgctactagta | 29 | 67 | 55 | ||
| P34 | IaaM_Kozak_BB_F | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGGTTCCAAACCATGCGCGAAATGATTACGC | 60 | 55/76 | 46/52 | ||
| P35 | IaaH_Kozak_BB_F | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGGTTCCAAACCatgtttggaccggatttcc | 60 | 54/76 | 47/52 |
Promoteurs
| Code | Name | Length | Concentration (ng/ul) | Species of origin | Expression domain | Restriction sites (EcoRI, XbaI, SpeI, PtsI) | Reference | Sequence |
| Promo 1 | p2 Xlurp | 5273bp | 177,1 | Xenopus Laevis | Myeloid cells | / | Smith et al., 2002 | Sequence
|
| Promo 2 | p2 CMV | 1016bp | 65,3 | Cytomegalovirus | Widespread | / | Werdien et al., 2001 | Sequence
|
| Promo 3 | p2 Flk-1 | 2723bp | 54 | Xenopus Laevis | Vasculature | / | Doherty et al., 2007 | Sequence
|
| Promo 4 | p2 14xUAS E1b-ATG | 454bp | 93,7 | Xba1 | Sequence
| |||
| Promo 5 | p2 ROSA26 | 814bp | 55,7 | Mus musculus | Widespread | / | Gross et al., 2006 | Sequence
|
| Promo 6 | p2 foxi short | 2020bp | 105,9 | Xenopus Tropicalis | Ionocytes | / | Unpublished results | Sequence
|
| Promo 7 | p2 UASt | 341bp | 64,8 | Saccharomyces cerevisiae | GAL4 inductible | / | Sequence | |
| Promo 8 | p2 HB9 | 3142bp | 48,1 | Danio rerio | Motor neurons | PtsI | Flanagan-Street et al., 2005 | Sequence |
| Promo 9 | p2 pax6 | bp | 58,9 | Sequence | ||||
| Promo 10 | p2 NBT | 3481bp | 168,7 | Xenopus Laevis | Differentiated neurons | EcoRI | Huang et et al., 2007 | Sequence |
| Promo 11 | p2 LMO2 long | 4730bp | 170,1 | Sequence | ||||
| Promo 12 | p2 pax3 | 2916bp | 109,5 | Xenopus Laevis | Neural ectoderm | Unpublished results | Sequence | |
| Promo 13 | p2 vimentin | 7205bp | 187,4 | Xenopus Tropicalis | Neural progenitor/glia | Love et al., 2011 | Sequence | |
| Promo 14 | p2 Brachyury | 3856bp | 103,6 | Sequence | ||||
| Promo 15 | p2 CMG long | 92,4 | Sequence | |||||
| Promo 16 | p2 LMO2 short | 2007bp | 88,3 | Sequence | ||||
| Promo 17 | p2 Ef1a | 528bp | 128,9 | Sequence | ||||
| Promo 18 | p2 Hsp70 | 487bp | 62,5 | Xenopus Laevis | Heat shock inductible | PrsI | Beck et al., 2003 | Sequence |
| Promo 19 | pCAR (eGFP) | bp | 2900 | Sequence |
Glycerol Stocks
| Number | Strain | Plasmid | Description | Date |
| P1 | E.Coli DH5 aplpha | pCS2+ | RFP ( BBA_J23100 ) | 13th july |
| P2 | E.Coli DH5 aplpha | pCS2+ | Auxine () | 13th july |
| P3 | E.Coli DH5 aplpha | pCS2+ | Tir1 GFP-Aid () | 19th july |
| P4 | E.Coli DH5 aplpha | pCS2+ | Tir1 GFP-Aid () | 19th july
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