Team:Uppsala University/Notebook

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Team Uppsala University – iGEM 2012

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Week 2

Tuesday 26 June

Participant: Pikkei Experiment: Re-streak from agar stabs
pSB1C3 - BBa_K322922 (cat + sacB) pSB1C3 - BBa_K322921 (sacB)	BBa_K157000 - BBa_K243000 (Fok_a) BBa_K157000 - BBa_K243001 (Fok_i)

Participant: Karl, Sabri, Pikkei Experiment: Take up from kit and transform
pSB1A2 - Plac (BBa_R0010) from plate 1, well 1D pSB2K3 - pBAD (BBa_I50500) from plate 1, well 14N	pSB1A2 - PlacQ (BBa_K091111) from plate 5, well 12C

Participant: Emy, Caroline Experiment: Start o/n culture
pSB1C3 - tetA pSB1C3 - CTX-M-15 pSB1C3 - ACC(6')_lb_cr pSB1C3 - SYFP2 pSB1C3 - red pSB3C5 - red pSB3T5 - red	pSB1A3 - red pSB4A5 - red pSB1A2 - red pSB1K3 - J23101 pSB1K3 - red pSB3K3 - red MG1655 pSB4K1 -lacIs (N246Q)

Wednesday 27 June

Participant: Pikkei
Experiment: Colony PCR and o/n culture

18. pSB1C3 – cat-sacB

19. pSB1C3 - sacB

20. BBa_K157000 – Fok_a

21. BBa_K157000 – Fok_i

Screened two clones of each
Left in PCR-machine o/n

Participant: Sabri, Fredrik
Experiment: Re-streak

15. pSB1A2 - Plac

17. pSB1A2 - PlacQ

Participant: Sibel, Lisa, Sabri, Olle, Hampus, Caroline, Emy, Joakim, Joel, Anna, Anders
Experiment: Plasmid Prep and Glycerol Stock

4. pSB1A3 - red

5. pSB4A5 - red

12. pSB1A2 - B0034

13. pSB1K3 - J23101

14. MG1655 - pSB4K1 - lacI (N246Q)

pSB1C3 - CTX-M-15

pSB1C3 - ACC(6')_lb_cr

pSB1C3 - SYFP2

pSB1K3 - red

pSB3K3 - red

pSB1C3 - tetA

1. pSB1C3 - red

2. pSB3C5 - red

3. pSB3T5 - red

Followed Plasmid Purification Protocol

Participant: Hampus, Sabri
Experiment: Cloning of 22. pEL3S15 - red and 13. pSB1K3 - J23101

30. pEL3S15 - J23101

Cut with X and P

Participant: Caroline, Emy, Ellen, Joel
Experiment: Assembly
pSB3T5 - lacIs - TλT1
pSB3T5 - SYFP2 - TλT1
pSB3T5 - T5lac - B0034
Followed BioBrick Assembly Manual

Thursday 28 June

Participant: Joel
Experiment: Gel Electrophoresis of yesterday's screening products
18.1-2 pSB1C3 – cat-sacB
19.1-2 pSB1C3 - sacB
20.1-2 BBa_K157000 – Fok_a
21.1-2 BBa_K157000 – Fok_i
Result: None of the band were correct

Participant: Caroline, Emy, Ellen, Joel
Experiment: Transformation
pSB3T5 - lacIs - TλT1
pSB3T5 - SYFP2 - TλT1
pSB3T5 - T5lac - B0034
Followed TOP10 transformation protocol

Participant: Fredrik, Julia, Joakim K, Olle
Experiment: Plasmid Prep and Glycerol Stock
18.1-2 pSB1C3 – cat-sacB
19.1-2 pSB1C3 - sacB
20.1-2 BBa_K157000 – Fok_a
21.1-2 BBa_K157000 – Fok_i
Followed Plasmid Purification Protocol

Participant: Lisa, Sabri
Experiment: o/n culture
.1-2 pSB1A2 - Plac
.1-2 pSB1A2 - PlacQ

Participant: Joakim, Anna
Experiment: Make new SOB & SOC

Participant: Tobias, Christoffer
Experiment: Transformation
pEL3S15 - J23101

Friday 29 June

Participant: Julia, Hampus, Tobias
Experiment: Re-streak
pEL3S15 - J23101

Participant: Olle, Sabri, Lisa
Experiment: Transformation
23. pSB1A1 - P1004 (cat) from registry well 7B
24. pSB2K3 - tetR from registry well 16P

Participant: Sabri, Lisa, Josefin, Niklas
Experiment: Assembly
pSB1K3 - B0034 - SYFP2
pSB3C5 - J23101 - tet inv

Participant: Caroline, Emy, Ellen, Joel, Tobias
Experiment: Assembly
pSB3T5 - PlacQ - ACC(6')
pSB3T5 - PlacQ - CTX-M-15
pSB3T5 - PlacQ - tetA
pSB3K3 - Plac - B0034 - SYFP2
pSB1C3 - TλT1

Participant: Caroline, Emy, Ellen, Joel, Tobias
Experiment: Re-streak
pSB3T5 - lacIs - TλT1
pSB3T5 - SYFP2 - TλT1
pSB3T5 - T5lac - B0034

Participant: Anna, Ellen
Experiment: o/n culture
J23116

Participant: Anna, Ellen
Experiment: Plasmid Prep
15.1-2 pSB1A2 - Plac
17.1-2 pSB1A2 - PlacQ

Saturday 30 June

Participant: Hampus, Julia, Tobias
Experiment: Screening
pEL3S15 - J23101
pSB3T5 - lacIs -
pSB3T5 - SYFP2 -
pSB3T5 - pT5lac - B0034
Result: Bad gel

Participant: Caroline, Fredrik, Emy, Hampus
Experiment: o/n culture
30.1-4 pEL3S15 - J23101
31.1-4 pEL3T5 - lacIs -
32.1-4 pSB3T5 - SYFP2 -
33.1-2 pSB3T5- pT5lac - B0034
20.1 BBa_K157000 - Fok_a
21.1 BBa_K157000 - Fok_i

Participant: Ellen, Emy, Caroline, Joel
Experiment: Plasmid Prep
J61002 - J23106
J61002 - J23116

Participant: Ellen, Emy, Caroline, Joel
Experiment: Transformation
pSB3T5 - PlacIq - AAC(6')
pSB3T5 - PlacIq - CTX-M
pSB3T5 - PlacIq - tetA
pSB3K3 - Plac - B0034 - SYFP2
pSB1C3 - T....

Participant: Ellen, Emy, Caroline, Joel
Experiment: Assembly
pSB3T5 - J23106 - AAC(6')
pSB3T5 - J23106 - CTX-M
pSB3T5 - J23106 - tetA
pSB3T5 - J23116 - AAC(6')
pSB3T5 - J23116 - CTX-M
pSB3T5 - J23116 - tetA

Participant: Fredrik
Experiment: Transformation
24. pSB2K3 - tet inv
39. pSB1K3 - B0034 - SYFP2
40. pSB3C5 - J23101 - tet inv

Participant: Fredrik
Experiment: Re-streak
16. pSB2K3 - pBAD
23. pSB1A1 - P1004
24. pSB1K3 - tet inv

Sunday 1 July

Participant: Hampus, Julia, Lisa
Experiment: Ran new gel of yesterday's screening
pEL3S15 - J23101
pSB3T5 - lacIs -
pSB3T5 - SYFP2 -
pSB3T5 - pT5lac - B0034
Result: Contamination

Participant: Caroline, Emy
Experiment: Transformation
pSB3T5 - J23106 - AAC(6')
pSB3T5 - J23106 - CTX-M
pSB3T5 - J23106 - tetA
pSB3T5 - J23116 - AAC(6')
pSB3T5 - J23116 - CTX-M
pSB3T5 - J23116 - tetA

Participant: Caroline, Emy
Experiment: Re-streak
pSB3T5 - PlacIq - AAC(6')
pSB3T5 - PlacIq - CTX-M
pSB3T5 - PlacIq - tetA
pSB3K3 - Plac - B0034 - SYFP2
pSB1C3 - T....

Participant: Emy, Caroline, Joel
Experiment: o/n culture
31.1-4 pSB3T5 - lacIs - T...
32.1-4 pSB3T5 - SYFP2 - T...
33.1-2 pSB3T5 - pT5lac - B0034
24.1-4 pSB1K3 - tet inv
30.1-4 pEL3S15 - J23101
20.1 BBa_K157000 - Fok_a
21.2 BBa_K157000 - Fok_i
23.1-4 pSB1A1 - P1004 (BBa_P1004)
44. pEL3C15 (FRT) - Red
45. pEL3C15 - Red
46. pEL3A15 - Red
47. pEL3A15 (FRT) - Red
48. pEL3S15 (FRT) - Red
49. pEL3S15 - Red
50. pEL3K15 (FRT) - Red
51. pEL3K15 - Red

Participant: Joel
Experiment: Transformation from kit
41. pSB2K3 - Pcat (BBa_I14033) from plate 1, well 18P
42. pSB2K3 - pBAD (BBa_I14018) from plate 1, well 18N
43. pSB2K3 - Pcat (BBa_I14031) from plate 2, well 13B

Week 1

Prepared TOP10 competent cells
Prepared LB medium, TEB medium and selective agarplates

Sponsors

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