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Contents 1 Columbia Copper Experiment Notebook 1.1 June, 2012 1.1.1 Week 1 1.1.1.1 Friday, 22nd 1.2 July, 2012

Columbia Copper Experiment Notebook

June, 2012

Week 1

Friday, 22nd

Outline of activity today:

• Met with Jason, who showed us how to make A. ferrooxidans liquid media, prepared Copper 1 and 2 samples (with and without bacteria) and placed on shaker. Will be periodically checked until June 25 twice a day for activity.
• Showed us recipe for solid media, and made enough for two samples, 20 mL (one control with no bacteria). Copper samples were submerged in agarose (see Steven’s pictures). Also learned protocols listed below.

Starting Sample masses:

Copper 1: (liquid media only)

Sample	Mass (g)
1	0.0092
2	0.0107.

Copper 2: (liquid media only)

Sample	Mass (g)
1	0.1318
2	0.1286.

Copper 2: (solid media)

Sample	Mass (g)
1	0.1272
2	0.1282.

Liquid media and flasks were placed in shaker at 11:35 am, 225 rpm, 30C. Solid media and petri dishes were placed in incubator at 12:42 pm, 30C.

July, 2012