Our protocols

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Pouring of plates

Bacillus subtilis Transformation

Storage of strains

Plasmid or Cosmid DNA Purification Using QIAGEN HiSpeed Plasmid Midi Kits

QIAquick PCR Purification Kit

Ligation

TSS chemical transformation

NucleoSpin Plasmid QuickPure : Isolation of high-copy plasmid DNA from E. coli

Tests on Staphylococcus aureus biofilms

Tests on Staphylococcus epidermidis biofilms in 24 well plate

Tests of Bacillus subtilis adherence in 24 well plate

OD₆₀₀ Test

Filling-in

SDS-PAGE

Plac kinetics analysis

Positive Biofilm test