Team:Lyon-INSA/protocol

From 2012.igem.org

Our protocols


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Pouring of plates
Bacillus subtilis Transformation
Storage of strains
Plasmid or Cosmid DNA Purification Using QIAGEN HiSpeed Plasmid Midi Kits
QIAquick PCR Purification Kit
Ligation
TSS chemical transformation
NucleoSpin Plasmid QuickPure : Isolation of high-copy plasmid DNA from E. coli
Tests on Staphylococcus aureus biofilms
Tests on Staphylococcus epidermidis biofilms in 24 well plate
Tests of Bacillus subtilis adherence in 24 well plate
OD600 Test
Filling-in
SDS-PAGE
Plac kinetics analysis
Positive Biofilm test
Confocal microscopy (surfactin test)
Surfactin test

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