Team:Michigan/Notebook/Protocols

From 2012.igem.org

Revision as of 02:23, 4 October 2012 by Cbate (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Xayona Website Template

Protocols

General

Plate Production</html>[1]

Cloning

Removing BioBricks from the Registry[https://static.igem.org/mediawiki/2012/5/50/MichRem.pdf]

Production of Competent Cells[2]

Heat Shock Transformation[3]

DNA Purification: PCR[4] DNA Purification: MiniPrep[5]

Restriction Enzyme Digest[6]

Ligation[7]

PCR (Phusion)[8]

Colony PCR[9]

Gel Electrophoresis[10]

Characterization

Transmission Electron Microscopy Protocol

FimE Asymmetrical Digest Assay Protocol[11]