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From 2012.igem.org

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Optical Density Measurement Protocol

1. Preset temperature and wavelength for measuring the optical density
2. Read and reference 1mL control solution (e.g. if E. coli was grown in LB/CAM) placed in disposable cuvette
3. Place 10µl cells into 1mL solution
4. Read the absorbance

Make note that when reading the optical density, must be referenced by a reference solution

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