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Team:Ehime-Japan/Safety
From 2012.igem.org
Safety
Q1
Would any of your project ideas raise safety issues in terms of researcher safety, public safety, or environmental safety?
A1
No. Our project ideas do not raise safety issues in terms of them. We used the light sensor genes from Synechocystis and the lon and the ssrAtag genes from Mesoplasma florum in E.co-mail and E.co-Domino. Also, the lux and las gene regularion systems were used in E.cold-heat sensing system. An explanation of the safety standard about our experiments is as follows.
In Japan, we must document our local committee which organism the gene is from and what the host isbefore we carry out gene recombination experiments. The organisms that the gene used in our projects are originated from are shown in Table1 with the Class as defined in Japanese ordinance that the organism are classified into. The hosts used in our experiments are shown in Table 2.
※Class1: All the organisms expect class 2to4.
Class2:The organisms having low virulence to mammals.
Classes3and4 can cause more risks.
Q2
Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes, did you document these issues in the Registry? How did you manage to handle the safety issue? How could other teams learn from your experience?
A2
No. Our new BioBrick parts are safe and all the genes we used are BL1 or BL2.
Q3
Is there a local biosafety group, committee, or review board at your institution? If yes, what does your local biosafety group think about your project? If no, which specific biosafety rules or guidelines do you have to consider in your country?
A3
Yes. Before we started the experiments, we submitted the safety list about our projects to our biosafety
committee, and it was accepted without problems.
Q4
Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?
A4
We think it would be good to make a check list in order to confirm whether projects or experimental equipment are proper. Also, in case of using a virulent organism or a gene from it, the user should show clearly how to deal with it. We can easily answer and find some problems we did not think.
