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Team:SUSTC-Shenzhen-B/lab results
From 2012.igem.org
Result
1. Fluorescence microscope results
Our plasmid design contains a RFP and GFP. Fluorescence microscope pictures (Figure 1 and Figure 2) show that both GFP and RFP are expressed.
(Figure 1: Those bacteria who express GFP and RFP are seen under the fluorescence microscope activated by 488nm light.The picture shows the expression of the RFP. )
(Figure 2: Those bacteria who express GFP and RFP are seen under the fluorescence microscope activated by 488nm light.The picture shows the expression of the GFP. )
2. Fit curves relating to dscores and terminator efficiencies.
(figure 3 : According the data on biofab ( http://io.biofab.org/services/studio/dac/ ) and our software predicted dscores, create fit curves. Linear fit curve is founded to have the highest accuracy among all the fit curves. )
(figure 4 : According to our experiment measured terminator efficiencies and our software predicted dscores, we create fit curve to relate all the data . To correspond to the figure 3, we choose linear fit curve to link all the data. There are 5 valuable points of terminator efficiencies. The sequences are listed below. In the experiment, we transform 9 terminators to plasmid mutant-psb1a3-GF and only get 5 reliably terminators’ data. The points appeared on the figure are terminators whose number is 1,4,6,7,8 )
(figure 5 : Here listed the sequences of terminator whose efficiencies are measured in the lab.)
(figure 5. Summary of results)
