Team:SJTU-BioX-Shanghai/Parts3

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Revision as of 03:28, 27 September 2012 by AleAlejandro (Talk | contribs)

Basic Parts of System

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771101 Membrabe Anchor 0 (BBa_K771101)]

12SJTU-SSdsbA-BLA.png

SsDsbA is the signal recognition particle (SRP)-dependent signaling sequence of DsbA. SsDsbA-tagged proteins are exported to the periplasm through the SRP pathway. With ssDsbA fused to the N-terminus, engineered membrane proteins are expected to be anchored onto inner membrane of E.coli . β-lactamase，a bacterial enzyme which must be exported to the periplasm in order to confer significant resistance to β-lactam antibiotics, such as ampicillin. Note that N-terminus of Lgt faces the periplasm and C-terminus faces the cytoplasm (Fig.3). Hence, if our fusion membrane protein is correctly anchored to membrane, β-lactamase is expected to be functional and host cells should be able to grow on culture media containing ampicillin.

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771102 LGT (BBa_K771102)]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771103 ssDsbA-PDZ Domain (BBa_K771103)] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K771104 ssDsbA-PDZ Ligand (BBa_K771104)]

File:12SJTU 2PDZ.gif

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771105 GBD Domain (BBa_K771105)] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K771106 GBD Ligand (BBa_K771106)]

File:12SJTU GBD.gif

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771107 SH3 Domain (BBa_K771107)] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K771108 SH3 Ligand (BBa_K771108)]

File:12SJTU SH3.gif

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771109 VVD(C71V and N56K) (BBa_K771109)]

VVD dark state.png

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771111 2xPP7 (BBa_K771111)] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K771112 MS2 (BBa_K771112)] and
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771009 RNA-d0(BBa_K771009)]

12SJTU RNASIGNAL.jpg

A: Sketch of signal RNA molecule (called D0) which consists of PP7 and MS2 aptamer domains. B: Through fusing PP7 and MS2 protein to our membrane device, RNA molecule in Figure A can function as a bridge to connect different proteins, thus decreasing distance between corresponding proteins

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771113 Split EGFP 1 (BBa_K771113)] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K771114 Split EGFP 2 (BBa_K771114)]




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