Team:Uppsala University/Notebook

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Team Uppsala University – iGEM 2012

Notebook

Thursday 28 June

Participant: Joel
Experiment: Gel Electrophoresis of yesterday's screening products
18.1-2 pSB1C3 – cat-sacB
19.1-2 pSB1C3 - sacB
20.1-2 BBa_K157000 – Fok_a
21.1-2 BBa_K157000 – Fok_i
Result: None of the band were correct

Participant: Caroline, Emy, Ellen, Joel
Experiment: Transformation
pSB3T5 - lacIs - TλT1
pSB3T5 - SYFP2 - TλT1
pSB3T5 - T5lac - B0034
Followed TOP10 transformation protocol

Participant: Fredrik, Julia, Joakim K, Olle
Experiment: Plasmid Prep and Glycerol Stock
18.1-2 pSB1C3 – cat-sacB
19.1-2 pSB1C3 - sacB
20.1-2 BBa_K157000 – Fok_a
21.1-2 BBa_K157000 – Fok_i
Followed Plasmid Purification Protocol

Participant: Lisa, Sabri
Experiment: o/n culture
.1-2 pSB1A2 - Plac
.1-2 pSB1A2 - PlacQ

Participant: Joakim, Anna
Experiment: Make new SOB & SOC

Participant: Tobias, Christoffer
Experiment: Transformation
pEL3S15 - J23101

Friday 29 June

Participant: Julia, Hampus, Tobias
Experiment: Re-streak
pEL3S15 - J23101

Participant: Olle, Sabri, Lisa
Experiment: Transformation
23. pSB1A1 - P1004 (cat) from registry well 7B
24. pSB2K3 - tetR from registry well 16P

Participant: Sabri, Lisa, Josefin, Niklas
Experiment: Assembly
pSB1K3 - B0034 - SYFP2
pSB3C5 - J23101 - tet inv

Participant: Caroline, Emy, Ellen, Joel, Tobias
Experiment: Assembly
pSB3T5 - PlacQ - ACC(6')
pSB3T5 - PlacQ - CTX-M-15
pSB3T5 - PlacQ - tetA
pSB3K3 - Plac - B0034 - SYFP2
pSB1C3 - TλT1

Participant: Caroline, Emy, Ellen, Joel, Tobias
Experiment: Re-streak
pSB3T5 - lacIs - TλT1
pSB3T5 - SYFP2 - TλT1
pSB3T5 - T5lac - B0034

Participant: Anna, Ellen
Experiment: o/n culture
J23116

Participant: Anna, Ellen
Experiment: Plasmid Prep
15.1-2 pSB1A2 - Plac
17.1-2 pSB1A2 - PlacQ

Saturday 30 June

Participant: Hampus, Julia, Tobias
Experiment: Screening
pEL3S15 - J23101
pSB3T5 - lacIs -
pSB3T5 - SYFP2 -
pSB3T5 - pT5lac - B0034
Result: Bad gel

Participant: Caroline, Fredrik, Emy, Hampus
Experiment: o/n culture
30.1-4 pEL3S15 - J23101
31.1-4 pEL3T5 - lacIs -
32.1-4 pSB3T5 - SYFP2 -
33.1-2 pSB3T5- pT5lac - B0034
20.1 BBa_K157000 - Fok_a
21.1 BBa_K157000 - Fok_i

Participant: Ellen, Emy, Caroline, Joel
Experiment: Plasmid Prep
J61002 - J23106
J61002 - J23116

Participant: Ellen, Emy, Caroline, Joel
Experiment: Transformation
pSB3T5 - PlacIq - AAC(6')
pSB3T5 - PlacIq - CTX-M
pSB3T5 - PlacIq - tetA
pSB3K3 - Plac - B0034 - SYFP2
pSB1C3 - T....

Participant: Ellen, Emy, Caroline, Joel
Experiment: Assembly
pSB3T5 - J23106 - AAC(6')
pSB3T5 - J23106 - CTX-M
pSB3T5 - J23106 - tetA
pSB3T5 - J23116 - AAC(6')
pSB3T5 - J23116 - CTX-M
pSB3T5 - J23116 - tetA

Participant: Fredrik
Experiment: Transformation
24. pSB2K3 - tet inv
39. pSB1K3 - B0034 - SYFP2
40. pSB3C5 - J23101 - tet inv

Participant: Fredrik
Experiment: Re-streak
16. pSB2K3 - pBAD
23. pSB1A1 - P1004
24. pSB1K3 - tet inv

Sunday 1 July

Participant: Hampus, Julia, Lisa
Experiment: Ran new gel of yesterday's screening
pEL3S15 - J23101
pSB3T5 - lacIs -
pSB3T5 - SYFP2 -
pSB3T5 - pT5lac - B0034
Result: Contamination

Participant: Caroline, Emy
Experiment: Transformation
pSB3T5 - J23106 - AAC(6')
pSB3T5 - J23106 - CTX-M
pSB3T5 - J23106 - tetA
pSB3T5 - J23116 - AAC(6')
pSB3T5 - J23116 - CTX-M
pSB3T5 - J23116 - tetA

Participant: Caroline, Emy
Experiment: Re-streak
pSB3T5 - PlacIq - AAC(6')
pSB3T5 - PlacIq - CTX-M
pSB3T5 - PlacIq - tetA
pSB3K3 - Plac - B0034 - SYFP2
pSB1C3 - T....

Participant: Emy, Caroline, Joel
Experiment: o/n culture
31.1-4 pSB3T5 - lacIs - T...
32.1-4 pSB3T5 - SYFP2 - T...
33.1-2 pSB3T5 - pT5lac - B0034
24.1-4 pSB1K3 - tet inv
30.1-4 pEL3S15 - J23101
20.1 BBa_K157000 - Fok_a
21.2 BBa_K157000 - Fok_i
23.1-4 pSB1A1 - P1004 (BBa_P1004)
44. pEL3C15 (FRT) - Red
45. pEL3C15 - Red
46. pEL3A15 - Red
47. pEL3A15 (FRT) - Red
48. pEL3S15 (FRT) - Red
49. pEL3S15 - Red
50. pEL3K15 (FRT) - Red
51. pEL3K15 - Red

Participant: Joel
Experiment: Transformation from kit
41. pSB2K3 - Pcat (BBa_I14033) from plate 1, well 18P
42. pSB2K3 - pBAD (BBa_I14018) from plate 1, well 18N
43. pSB2K3 - Pcat (BBa_I14031) from plate 2, well 13B

Monday 9 July

Participant: Hampus, Christofer
Experiment: Screening
33. pSB1A3 - PT5lac - B0034
80. pSB1C3 - J23110 - B0034
81. pSB1C3 - J23116 - B0034
Result: 33, 80 and 81 showed correct bands.

Participant: Anton, Joakim, Karl
Experiment: Prepare for sequencing
40. pSB3C5 - J23101 - tet inv.
64 pEL3S15 - J23101 - Spot42

Experiment: O/N
82. pSB1AK3 - Plac - B0034
12. pSB1A2 - B0034
83. pUC57 - TλT1

Experiment: Assembly
39. pSB1K3 - B0034 - SYFP2

Experiment: Cloning
88. pSB1C3 - Pcat

Participant: Emy, Ellen
Experiment: Assembly and transformation
109. pEL3A15 - PlacIq - AAC(6') - lacIs
110. pEL3A15 - PlacIq - CTX-M - lacIs
111. pEL3A15 - PlacIq - tet-A - lacIs

Participant: Lisa, Sabri
Experiment: Plasmid prep.
64. pEL3S15 - J23101 - Spot42

Participant: Julia, Hampus
Experiment: Amplify SpecA-casette from pEL3S15 - J23101 - Spot42

Tuesday 10 July

Participant: Joel Ås
Experiment: Transform
109. pEL3A15- PlacIq - AAC(6') - lacIs
110. pEL3A15 - PlacIq - CTX-M - lacIs
111. pEL3A15 - PlacIq - tetA - lacIs

Participant: Anna, Emy
Experiment: Assembly and Transformation
113. pSB3T5 - J23110 - AAC(6')
114. pSB3T5 - J23110 - CTX-M

Participant: Anna, Emy, Karl
Experiment: Streak from frozen stock
9. pSB1C3 - CTX-M

Experiment: O/N from frozen stock
3. pSB3T5 - red

Participant: Olle
Experiment: Assembly
59. pSB3T5 - J23116 - tetA
115. pSB3T5 - J23110 - tetA

Experiment: Transform
59. pSB3T5 - J23116 - tetA
91. pSb1C3 - marR
115. pSB3T5 - J23110 - tetA

Participant: Arvid
Experiment: Control experiment
Since we've had problems growing pSB3K3-pBAD/acaC on a K-backbone, we tested some different antibiotics
Result: Someone probably mixed up the sample at an early stage since it grew in ampicillin.

Experiment: Plasmid prep and Frozen Stock
12. pSB1A2 - B0034
33. pSB3T5 - T5lac - B0034
80. pSB1C3 - J23110 - B0034
81. pSB1C3 - J23116 - B0034
82. pSB1AK3 - Plac - B0034
83. pUC57 - TλT1

Participant: Anton Berglund
Experiment: Transformation
39. pSB1K3 - B0034 - SYFP2
88. pSB1C3 - Pcat

Experiment: Prep for sequencing
33. pSB3T5 - PT5lac - B0034
80. pSB1C3 - J23110 - B0034
81. pSB1C3 - J23116 - B0034
82. pSB1AK3 - Plac - B0034
84. pSB3T5 - cat-sacB

Participant: Johan
Experiment: re-streak
pSB1C3 - marR (BioBricked)

Wednesday 11 July

Participant: Anton, Olle
Experiment: re-streak
39. pSB1K3 - B0034 - SYFP2
88. pSB1C3 - pcat

Participant: Joel Ås
Experiment: screening
ImmR
Pxis

Participant: Hampus, Julia
Experiment: Transformering
pEL3S15 - J23101 - B0034 - amilCP

Experiment: Re-streak
64. pEL3S15 - J23101 - Spot42

Participant: Ellen, Emy
Experiment: Assembly
113. pSB3T5 - J23110 - AAC(6')
53. pSB3K3 - Plac - B0034 - SYFP2

Experiment: Cloning
52. pSB1C3 - TλT1

Thursday 12 July

Participant: Hampus, Julia
Experiment: Screening
64. pEL3S15 - J23101 - Spot42
Result: Clone 2 and 7 showed correct band (1500 bp).

Participant: Anton
Experiment: Re-streak
39. pSB1K3 - B0034 - SYFP2

Participant: Johan
Experiment: Screening
pSB1C3 - marR_BB10
Result: Wrong band.

Participant: Fredrik
Experiment: Ligation
131. pSB1C3 - ImmR
132. pSB1C3 - Pxis

Participant: Olle, Karl
Experiment: Make some beautiful competent cells
Result: Approximate 250 competent cells in our -80 freezer.

Participant: Emy, Ellen
Experiment: Screening
55. pSB3T5 - J23106 - CTX-M
58. pSB3T5 - J23116 - CTX-M
59. pSB3T5 - J23116 - tetA
110. pEL3A15 - PlacQ - CTX-M - lacIs
111. pEL3A15 - PlacQ - tetA - lacIs
115. pSB3T5 - J23110 - tetA
Result: All bad.

Participant: Anna, Fredrik
Experiement: Screening
marR

Experiement: Assembly
112. pSB1C3 - marR

Participant: Joel
Experiment: Transformation
121. pEL3A18 - red
122. pEL3C18 - red
123. pEL3K18 - red

Experiment: PCR purification and digestion
Pxis
ImmR

Participant: Lisa, Sabri
Experiement: Randomized PCR on pEL3S15 - J23101 - Spot42

Friday 13 July

Participant: Johan
Experiement: Glycerol stock and plasmid prep.
marR

Participant: Karl
Experiment: Restreak
121. pEL3A18 - red
122. pEL3C18 - red
123. pEL3K18 - red

Participant: Anna, Fredrik
Experiement: Assembly and transformation

93. pSB3C5 - J23110 - tet inv.
94. pSB3C5 - J23106 - tet inv.
95. pSB3C5 - J23101 - tet inv.
96. pSB1C3 - J23106 - B0034
97. pSB1C3 - J23110 - B0034
98. pSB1C3 - J23116 - B0034
99. pSB3T5 - B0034 - SYFP2

Participant: Lisa, Sabri
Experiement: Screening
64. pEL3S15 - J23101 - Spot42

Participant: Olle, Joakim
Experiment: Transformation
52. pSB1C3 - TλT1
53. pSB3T5 - Plac - B0034 - SYFP2
88. pSB1C3 - pCat
113. pSB3T5 - J23110 - AAC(6')
126. pEL3S18 - red
127. pEL3A18 - red(FRT)
128. pEL3C18 - red(FRT)
129. pEL3K18 - red(FRT)
130. pEL3S18 - red(FRT)
123. pEL3A18 - red
124. pEL3C18 - red
125. pEL3K18 - red
131. pSB1C3 - ImmR
132. pSB1C3 - Pxis

Participant: Hampus, Julia, Christoffer
Experiment: Screening, PCR purification, DPNI treatment
specR - J23101
Result: Gave correct bands.

Participant: Anna, Fredrik
Experiment: Nested PCR and screening
OmpF
LexA
Result: LexA good, OmpF has to be redone.

Experiment: Nested PCR
OmpF

Experiment: BioBrick PCR
LexA
OmpF

Participant: Ellen, Emy
Experiment: Screening
55. pSB3T5 - J23106 - CTX-M
58. pSB3T5 - J23116 - CTX-M
59. pSB3T5 - J23116 - tetA
110. pEL3A15 - PlacQ - CTX-M - lacIs
111. pEL3A15 - PlacQ - tetA - lacIs
115. pSB3T5 - J23110 - tetA
Result : 58, 59 wrong, 55 showed no band, 109, 11 and 155 were right.
Experiment: O/N
109. pEL3A15 - PlacIQ - AAC(‘6) - lacIs
111. pEL3A15 -PlacIQ - tetA - lacIs
115. pSB3T5 - J23110 - tetA

Saturday 14 July

Participant: Karl
Experiment: Plasmid prep, glycerol stock
39. pSB1K3 - B0034 - SYFP2
109. pEL3A15 - PlacQ - AAC(6') - lacIs
111. pEL3A15 - PlacIQ - AAC(6') - lacIs
115. pSB3T5 - J23110 - tetA

Experiment: O/N from frozen stock
3. pSB3T5 - red

Participant: Ellen
Experiment: Assembly
133. pSB1A3 - J23106 - tetA
134. pSB1A3 - J23116 - tetA
139. pSB3T5 - PT5lac - B0034 - SYFP2
55. pSB3T5 - J23116 - CTX-M
58. pSB3T5 - J23106 - CTX-M

Experiment: Cloning
62. pSB1C3 - AmpR

Participant: Olle
Experiment: Restreak
126. pEL3S18
127. pEL3A18(FRT)
128. pEL3C18(FRT)
129. pEL3K18(FRT)
130. pEL3S18(FRT)
109. pEL3A15 - PlacIQ - AAC(6')
111. pEL3A15 - PlacIQ - tetA
115. pSB3T5 - J23110 - tetA

Experiment: Transformation
113. pSB3T5 - J23110 - AAC(6')
114. pSB3T5 - J23110 - CTX-M

Participant: Joel
Experiment: Cloning
132. pSB1C3 - Pxis

Participant: Anna, Fredrik
Experiment: Assembly and transformation
79. pSB1C3 - J23106 - B0034
80. pSb1C3 - J23110 - B0034
81. pSB1C3 - J23116 - B0034

Experiment: PCR purification, digestion and ligation into pSB1C3
OmpF
LexA

Experiment: Restreak
93. pSB3C5 - J23110 - tet inv.
94. pSB3C5 - J23106 - tet inv.
95. pSB3C5 - J23101 - tet inv.
96. pSB3T5 - B0034 - SYFP2

Experiment: Screening
OmpF
OmpF (BioBricked)
LexA (BioBricked)

Participant: Emy
Experiment: Restreak
52. pSb1C3 - TλT1
88. pSB1C3 - pCat
131. pSB1C3 - ImmR
60. pSB1A3 - B0032 - SYFP2
157. pSB1C3 - J23101 - B0034

Experiment: O/N
123 pEL3A18 - red
124. pEL3C18 - red
125. pEL3K18 - red

Participant: Arvid
Experiment: Assembly
101. pSB3T5 - J23110 - B0034 - SYFP2

Experiment: Cloning
140. pSB1A5 - B0034 - SYFP2
141. pSB1C5 - B0034 - SYFP2
142. pSB3C3 - B0034 - SYFP2
143. pSB3T3 - B0034 - SYFP2
144. pSB3A15 - B0034 - SYFP2
145. pSB3C15 - B0034 - SYFP2

Participant: Joakim
Experiment: Assembly
53. pSB3T5 - Plac - B0034 - SYFP2

Sunday 15 July

Participant: Joel
Experiment: Ligate
132. pSB1C3 - T0

Participant: Ellen
Experiment: Transformation
55. pSB3T5 - J23106 - CTX-M
58. pSB3T5 - J23116 - CTX-M
62. pSB1C3 - AmpR
132. pSB1C3 - Pxis
134. pSB1A3 - J23116 - tetA
139. pSB3T5 - PT5lac - B0034 - SYFP2
147. pSB1C3 - OmpF
148. pSB1C3 - LexA

Participant: Arvid
Experiment: Transformation
100. pSB1AK3 - B1002
101. pSB3T5 - J23110 - B0034 - SYFP2
140. pSB1A5 - B0034 - SYFP2
141. pSB1C5 - B0034 - SYFP2
142. pSB3C3 - B0034 - SYFP2
143. pSB3T3 - B0034 - SYFP2
144. pSB3A15 - B0034 - SYFP2
145. pSB3C15 - B0034 - SYFP2

Experiment: Restreak
53. pSB3T5 - Plac - B0034 - SYFP2

Participant: Anna, Fredrik
Experiment:
93. pSB3C5 - J23110 - tet inv.
94. pSB3C5 - J23106 - tet inv.
95. pSB3C5 - J23101 - tet inv
96. pSB3T5 - B0034 - SYFP3

Participant: Fredrik
Experiment: Assembly
79. pSB1C3 - J23106 - B0034
80. pSB1C3 - J23110 - B0034
81. pSB1C3 - J23116 - B0034

Participant: Caroline, Emy
Experiment: Screening
52. pSB1C3 - TλT1
131. pSB1C3 - ImmR

Experiment: O/N
123. pEL3A18 - red
124. pEL3K18 - red
131. pSB1C3 - ImmR

Participant: Hampus, Olle
Experiment: Mutagenesis - insertion of random nucleotides into pEL3S15 - J23101 - Spot42

Experiment: Screening
111. pEL3A15 - PlacIQ - tetA
115. pSB3T5 - J23110 - tetA

Participants: Olle
Experiment: Restreak
109. pEL3A15 - PlacIQ - AAC(6')
113. pSB3T5 - J23110 - AAC(6')
114. pSB3T5 - J23110 - CTX-M

Participant: Joel, Tobias
Experiment: Assembly
133. pSB1A3 - J23106 - tetA
134. pSB1A3 - J23116 - tetA

Experiment: Transformation
116. pEL3C18 - J23116 - AAC(6') - lacIs
117. pEL3A18 - PlacIQ - AAC(6') - SYFP2
118. pEL3A18 - PlacIQ - CTX-M - SYFP2
119. pEL3A18 - PlacIQ - tetA - SYFP2
120. pEL3A18 - J23106 - tetA - SYFP2
121. pEL3A18 - J23106 - CTX-M - SYFP2
122. pEL3C18 - J23106 - AAC(6') - lacIs
123. pEL3C18 - J23106 - tetA - lacIs
136. pEL3C18 - PlacIQ - AAC(6') - lacIs
137. pEL3C18 - PlacIQ - tetA - lacIs
138. pEL3C18 - PlacIq - CTX-M - lacIs

VECKA 5

Monday 16 July

Participant: Sabri, Lisa
Experiment: Mutagenesis - sRNA library
Result: No band on gel.

Participant: Caroline, Katarina
Experiment: Restreak
52. TλT1
55. pSB3T5 - J23106 - CTX-M
58. pSB3T5 - J23116 - CTX-M
133. pSB1A3 - J23106 - tetA
134. pSB1A3 - J23116 - tetA

Experiment: O/N
52. TλT1
113. pSB3T5 - J23110 - AAC(6')
114. pSB3T5 - J23110 - CTX-M

Participant: Olle, Arvid
Experiment: Restreak
140. pSB1A5 - B0034 - SYFP2
143. pSB3T5 - B0034 - SYFP2
144. pEL3A15 - B0034 - SYFP2
145. pEL3C15 - B0034 - SYFP2

Participant: Anton
Experiment: Assembly
101. pSB3T5 - J23110 - B0034 - SYFP2

Experiment: Prepare for sequencing
39.1,2 pSB1K3 - B0034 - SYFP2
93.2,4 pSB3C5 - J23110 - tet inv.
95.4 pSB3C5 - J23101 - tet inv.
115.2,3 pSB3T5 - J23110 - tetA
131.3,4 pSB1C3 - ImmR

Participant: Karl
Experiment: Restreak
147. pSB1C3 - LexA
148. pSB1C3 - OmpF

Participant: Julia
Experiment: O/N
96. pSB3T5 - B0034 - SYFP2

Participant: Julia, Hampus
Experiment: Restreak
MG1655 - SpecR - J23101

Participant: Olle
Experiment: Screening
52. TλT1
113. pSB3T5 - J23110 - AAC(6')
114. pSB3T5 - J23110 - CTX-M
14. pSB4K5 - lacIs
109. pEL3A15 - PlacIQ - AAC(‘6) - lacIs
Result: All good.

Participant: Joel, Joakim

Experiment: Plasmid prep.
131. pSb1C3 - ImmR
115. pSB3T5 - J23110 - tetA

Participant: Anna, Anders
Experiment: Plasmid prep.
93. pSB3C5 - J23110 - tet inv.
95. pSb3C5 - J23101 - tet inv.
96. pSB3T5 - B0034 - SYFP2
9. pSB1C3 - CTX-M
124. pEL3C18 - red

Participant: Karl
Experiment: Transformation
79. pSB1C3 - J23106 - B0034
80. pSB1C3 - J23110 - B0034
81. pSB1C3 - J23116 - B0034
101. pSB1T5 - J23110 - B0034 - SYFP2

Participant: Julia, Hampus
Experiment: Plasmid prep. and glycerol stock
123. pEL3A18 - red
125. pEL3K18 - red
126. pEL3S18 - red
127. pEL3A18(FRT) - red
128. pEL3C18(FRT) - red
129. pEL3K18(FRT) - red
130. pEL3S18(FRT) - red

Participant: Arvid
Experiment: Restreak
53. pSB3T5 - Plac - B0034 - SYFP2
100. pSB1AK3 - B1002

Tuesday 17 July

Participant:Lisa, Sabri
Experiment: Mutagenesis and screening of randomized sRNA library
Result: No visible bands on gel.

Participant: Hampus, Julia
Experiment: Screening
MG1655 - SpecR - J23101 - Cam
MG1655 - SpecR - J23101 - Tet
134. pSB1A3 - J23116 - tetA
133. pSB1A3 - J23106 - tetA
Result: 133, 134 correct length. Preparation of the other two was done incorrectly, no bands.

Participant:Johan, Anders
Experiment: Screening
52. TλT1
55. J23106 - CTX-M
14. lacIs
112. pSB1C3 - marR
Result: Screening has to be redone.

Participant: Caroline, Katarina
Experiment: Transformation
116. pEL3C18 - J23116 - AAC(6') - lacIs
117. pEL3A18 - PlacIQ - AAC(6') - SYFP2
118. pEL3A18 - PlacIQ - CTX-M - SYFP2
119. pEL3A18 - PlacIQ - tetA - SYFP2
120. pEL3A18 - J23106 - AAC(6') - SYFP2
121. pEL3A18 - J23106 - tetA - SYFP2
133. pSB1A3 - J23106 - AAC
134. pSB1A3 - J23116 - tetA
136. pEL3C18 - PlacIQ - AAC(6') - lacIs
137. pEL3C18 - PlacIQ - CTX-M - lacIs
138. pEL3C18 - PlacIQ - tetA - lacIs
146. pSB1C3 - T0
151. pEL3C18 - J23106 - AAC(6') - lacIs
152. pEL3C18 - J23106 - tetA - lacIs

Experiment: Assembly
62. pSB1C3 - AmpR
132. pSB1C3 - Pxis
139. pSB3T5 - T5lac - B0034 - SYFP2

Participant: Olle
Experiment: Screening
53. pSB3K3 - Plac - B0034 - SYFP2
100. pSB1AK3 - B1002

Participant: Karl
Experiment: Restreak
101. pSB3T5 - J23110 - B0034 - SYFP2

Participant: Anna
Experiment: Screening
147. pSB1C3 - LexA
148. pSB1C3 - OmpF
Result: OmpF might be good, LexA will be redone.

Participant: Anton
Experiment: Plasmid prep.
55. pSB3T5 - J23106 - CTX-M
Result: Too low concentration to use.

Experiment: Preparation for sequencing
52. pSB1C3 - TλT1
113. pSB3T5 - J23110 - AAC(6')
114. pSB3T5 - J23110 - CTX-M

Participant: Joakim

Experiment: Plasmid prep.
52. pSB1C3 - TλT1
53. pSB3K3 - Plac - B0034 - SYFP2
113. pSB3T5 - J23110 - AAC(6')
114. pSB3T5 - J23110 - CTX-M

Wednesday 18 July

Participant: Arvid, Anton
Experiment: Test if chloramphenicol can increase plasmid yield, O/N of:
140. pSB1A5 - B0034 - SYFP2
143. pSB3T5 - B0034 - SYFP2
with and without addition of chloramphenicol two hours before plasmid preparation.
Result: Slight increase, less than expected.

Participant: Emy, Caroline, Katarina, Joel
Experiment: Screening
55. pSB3T5 - J23106 - CTX-M
58. pSB3T5 - J23116 - CTX-M
133. pSB1A3 - J23106 - tetA
134. pSB1A3 - J23116 - tetA
lacIs PCR product
Result: 134 was wrong, the others had clones with inserts of correct lengths.

Participant: Anton
Experiment: Plasmid prep.
52. pSB1C3 - TλT1
55. pSB3T5 - J23106 - CTX-M
100. pSB1AK3 - B1002
148. pSB1C3 - OmpF

Participant:Lisa, Sabri
Experiment: Mutagenesis PCR of randomized sRNA library
Result: Failed!

Participant: Julia, Hampus
Experiment: Screening
MG1655 - SpecR - J23101 - Cam
MG1655 - SpecR - J23101 - Tet
Result: Both were of the right length.

Experiment: O/N
101. pSB3T5 - J23110 - B0034 - SYFP2
148. pSB1C3 - OmpF
149. MG1655 - SpecR - J23101 - Tet
150. MG1655 - SpecR - J23101 - Cam

Participant: Anna
Experiment: Cloning and transformation
156. pJN3C1 - AmpR

Participant: Anna, Joakim
Experiment: Transformation
62. pSB1C3 - AmpR
94. pSB3C5 - J23106 - tet inv.
132. pSB1C3 - Pxis
139. pSB3T5 - PT5lac - B0034 - SYFP2
147. pSB1C3 - LexA
lacI (from kit)

Thurusday 19 July

Participant: Caroline, Katarina, Emy
Experiment: Screening
53. pSB3T5 - Plac - B0034 - SYFP2
118. pEL3A18 - PlacIQ - CTX-M - SYFP2
119. pEL3A18 - PlacIQ - tetA - SYFP2
121. pEL3A18 - J23106 - tetA - SYFP2
134. pSB1A3 - J23116 - tetA
146. pSB1C3 - T0

Experiment: O/N
53. pSB3T5 - Plac - B0034 - SYFP2
58. pSB3T5 - J23116 - CTX-M
119. pEL3A18 - PlacIQ - tetA - SYFP2
146. pSB1C3 - T0

Experiment: Restreak
118. pEL3A18 - PlacIQ - CTX-M - SYFP2

Participant: Sabri, Lisa
Experiment: Mutagenesis of randomized sRNA library. DMSO added to prevent primers from folding secondary structures.

Participant: Joakim
Experiment: Restreak
94. pSB3C5 - J23106 - tet inv.
139. pSB3T5 - Pt5lac - SYFP2
155. pSB1A3 - lacI

Participant: Anna
Experiment: Screening
148. pSB1C3 - OmpF
11. pSB1C3 - SYFP2 and 24. pSB2K3 - tet inv. to see if they have been mixed

Experiment: Assembly
132. pSB1C3 - Pxis
156. pJN3C1 - AmpR

Participant: Joel
Experiment: Screening
55. pSB3T5 - J23116 - CTX-M
14. lacIs (PCR product)
Result: 55 wrong, lacIs correct length.

Participant: Arvid, Anton
Experiment: Screening, plasmid prep. and glycerol stock
101. pSB3T5 - J23110 - B0034 - SYFP2

Experiment: Transformation
158. pSB4K5 - red

Participant: Julia
Experiment: PCR purification
149. MG1655 - SpecR - J23101 - Tet

Participant: Anders, Johan
Experiment: Screening
116. pEL3C18 - J23116 - AAC(6') - lacIs
117. pEL3A18 - PlacIQ - AAC(6') - SYFP2
120. pEL3A18 - AAC(6') - SYFP2
136. pEL3C18 - PlacIQ - AAC(6') - lacIs
146. pSB1C3 - T0
151. pEL3C18 - J23106 - AAC(6') - lacIs
Result: Unknown.

Friday 20 July

Participant: Emy, Katarina, Caroline, Joel
Experiment: Screening
116. pEL3C18 - J23116 - AAC(6') - lacIs
117. pEL3A18 - PlacIQ - AAC(6') - SYFP2
120. pEL3A18 - AAC(6') - SYFP2
136. pEL3C18 - PlacIQ - AAC(6') - lacIs
146. pSB1C3 - T0
151. pEL3C18 - J23106 - AAC(6') - lacIs
Result: 116, 120, 136 good!

Experiment: Assembly
55. pSB3Tt5 - J23106 - CTX-M
121. pEL3A18 - J23106 - tetA - SYFP2
134. pSB1A3 - J23116 - tetA

Experiment: Screening
53. pSB3K3 - Plac - B0034 - SYFP2
58. pSB3Tt5 - J23116 - CTX-M
119. pEL3A18 - PlacIQ - tetA - SYFP2
Result: 53 and 58 had the right length.

Experiment: Plasmid prep.
53. pSB3K3 - Plac - B0034 - SYFP2
58. pSB3T5 - J23116 - CTX-M
119. pEL3A18 - PlacIQ - tetA - SYFP2

Participant: Joakim, Anna
Experiment: Screening
70. pSB3C5 - J23106 - tet inv.
139. pSB3T5 - Pt5lac - B0034 - SYFP2
147. pSB1C3 - LexA
155. pSB1A3 - lacI
Result: RESULTAT RESULTAT RESULTAT RESULTAT

Participant: Joel
Experiment: PCR amplification of lacIs with RBS and promoter removed. Screened on
gel.
Result: PCR failed.

Participant: Sabri, Lisa
Experiment: Screening
pEL3S15 - J23101 - random sRNA - Spot42
Result: 10% DMSO showed a band on the gel. New PCR with higher concentrations of
DMSO will be made.

Participant: Anna
Experiment: Transformation
55. pSB3Tt5 - J23106 - CTX-M
115. pSB3T5 - J23110 - tetA
121. pEL3A18 - J23106 - tetA - SYFP2
134. pSB1A3 - J23116 - tetA

Participant: Anders, Johan
Experiment: Screening
101. pSB3T5 - J23110 - B0034 - SYFP2
116. pEL3C18 - J23116 - AAC(6') - lacIs
117. pEL3A18 - PlacIQ - AAC(6') - SYFP2
120. pEL3A18 - AAC(6') - SYFP2
136. pEL3C18 - PlacIQ - AAC(6') - lacIs
146. pSB1C3 - T0
151. pEL3C18 - J23106 - AAC(6') - lacIs
Result: Gels failed. T0 seemed right.

Participant: Julia
Experiment: Restreak
137. pEL3C18 - PlacIQ - tetA - lacIs
138. pEL3C18 - PlacIQ - CTX-M - lacIs
152. pEL3C18 - J23106 - tetA - lacIs

Experiment: Glycerol stock
149. MG1655 - SpecR - J23101 - Tet

Saturday 21 July

Participant: Caroline, Katarina, Emy
Experiment: Screening
116. pEL3C18 - J23116 - AAC(6') - lacIs
117. pEL3A18 - PlacIQ - AAC(6') - SYFP2
120. pEL3A18 - AAC(6') - SYFP2
136. pEL3C18 - PlacIQ - AAC(6') - lacIs
146. pSB1C3 - T0
151. pEL3C18 - J23106 - AAC(6') - lacIs
Result: 116, 120 and 136 ok, the others were wrong.

Experiment: O/N
116. pEL3C18 - J23116 - AAC(6') - lacIs
120. pEL3A18 - AAC(6') - SYFP2
136. pEL3C18 - PlacIQ - AAC(6') - lacIs

Experiment: Assembly
117. pEL3A18 - PlacIQ - AAC(6') - SYFP2
118. pEL3A18 - PlacIQ - CTX-M - SYFP2

Experiment: Restreak
119. pEL3A18 - PlacIQ - tetA - SYFP2
137. pEL3C18 - PlacIQ
146. pSB1C3 - T0
152. pEL3C18 - J23106 - tetA - lacIs

Participant: Julia
Experiment: Screening
137. pEL3C18 - PlacIQ - CTX-M - lacIs
138. pEL3C18 - J23106 - lacIs
152. pEL3C18 - PlacIQ - tetA - lacIs
Result: 137 and 152 good.

Experiment: Restreak
137. pEL3C18 - PlacIQ - CTX-M - lacIs
152. pEL3C18 - PlacIQ - tetA - lacIs

Sunday 22 July

Participant: Anna
Experiment: Screening
139. pSB3T5 - PT5lac - B0034 - SYFP2
147. pSB1C3 - LexA

Participant: Joel, Emy, Caroline, Katarina, Karl, Pikkei
Experiment: Screen
115. pSB3T5 - J23110 - tetA
119. pEL3A18 - PlacIQ - tetA - SYFP2

Monday 23 July

Participant: Tobias, Christoffer
Experiment: PCR cloning of cat-sacB with λ primers for chromosomal integration

Participant: Sabri, Lisa
Experiment: Mutagenesis, with different concentrations of DMSO
pEL3S15 - J23101 - random sRNA - Spot42

Experiment: Screening
pEL3S15 - J23101 - random sRNA - Spot42
Result: Gel failed!

Participant: Olle
Experiment: Screening
159. pSB1C3 - pLlacO - B0034
160. pSB1C3 - T5lac - B0034
161. pSB1C3 - PrecA - B0034 - SYFP2
Result: All ok!

Participant: Joel, Ellen, Karl
Experiment: Phosphorylation of primers
lacIs_linker_CTX_F
B0034(mod)_R

Experiment: Mutagenesis
lacIs

Experiment: Plasmid prep.
115. pSB3T5 - J23110 - tetA
119. pEL3A18 - PlacIQ - tetA - SYFP2
121. pEL3A18 - J23106 - tetA - SYFP2
132. pSB1C3 - Pxis

Experiment: O/N
53. pSB3K3 - Plac - B0034 - SYFP2
58. pSB3T5 - J23116 - CTX-M
115. pSB3T5 - J23110 - tetA
119. pEL3A18 - PlacIQ - tetA - SYFP2
146. pSB1C3 - T0
158. pSB4K5 - red

Participant: Fredrik
Experiment: Plasmid prep.
55. pSB3T5 - J23106 - CTX-M
139. pSB3T5 - PT5lac - B0034 - SYFP2
147. pSB1C3 - LexA

Participant: Joakim, Anna
Experiment: Screening
80. pSB1C3 - J23110 - B0034
Result: OK band present on gel

Participant: Julia, Hampus
Experiment: Assembly
114. pSB1K3 - Pxis - B0034 -SYFP2
115. pEL3S15 - ImmR - λT1

Participant: Anton
Experiment: Preparation for sequencing
53. pSB3K3 - Plac - B0034 - SYFP2
55. pSB3T5 - J23106 - CTX-M
116. pEL3C18 - J23116 - AAC(6') - lacIs
120. pEL3A18 - J23106 - AAC(6') - SYFP2
136. pEL3C18 - PlacIQ - AAC(6') - lacIs
147. pSB1C3 - LexA
148. pSB1C3 - OmpF
149. MG1655 - SpecR - J23101

Participant: Arvid, Karl
Experiment: Screening
101. PSB3T5 - J23110 - B0034 - SYFP2
Result: All clones good!

Participant: Karl
Experiment: Assembly
134. pSB1A3 - J23116 - tetA

Experiment: Transformation
134. pSB1A3 - J23116 - tetA
162. pEL3A18 - J23110 - AAC(6') - SYFP2
163. pEL3A18 - J23110 - CTX-M - SYFP2

Experiment: Screening
60. pSB1A2 - B0032 - SYFP2
Result: Three out of six clones had the right length

Tuesday 24 July

Participant: Caroline, Katarina, Emy
Experiment: Screening
117. pEL3A18 - PlacIQ - AAC(6') - SYFP2
118. pEL3A18 - PlacIQ - CTX-M - SYFP2
Result: Both had clones with correct lengths

Experiment: Assembly and transformation
166. pEL3C18 - PlacIQ - AAC(6') - lacI
167. pEL3C18 - PlacIQ - CTX-M - lacI
168. pEL3C18 - PlacIQ - tetA - lacI
169. pEL3C18 - J23106 - AAC(6') - lacI
170. pEL3C18 - J23106 - tetA - lacI

Experiment: Transformation
pBad
AmpR
33. pSB3T5 - PT5lac - B0034
101. pSB3C5 - J23110 - B0034 - SYFP2
172. pSB3C5 - tet inv. - B0034 - SYFP2

Participant: Joel, Hampus, Julia
Experiment: Plasmid prep.
53. pSB3K3 - Plac - B0034 - SYFP2
58. pSB3T5 - J23116 - CTX-M
119. pEL3A18 - PlacIQ - tetA - SYFP2
Result: Concentration on 58 was too low for sequencing

Participant: Anton, Joakim
Experiment: Preparation for sequencing
53. pSB3K3 - Plac - B0034 - SYFP2
55. pSB3T5 - J23106 - CTX-M
101. pSB3C5 - J23110 - B0034 - SYFP2
116. pEL3C18 - J23116 - AAC(6') - lacIs
120. pEL3A18 - J23106 - AAC(6') - SYFP2
132. pSB1C3 - Pxis
136. pEL3C18 - PlacIQ - AAC(6') - lacIs
139. pSB3T5 - PT5lac - B0034 - SYFP2
147. pSB1C3 - LexA
149. MG1655 - SpecR - J23101
159. pSB1C3 - pLlacO - B0034
160. pSB1C3 - T5lac - B0034
161. pSB1C3 - PrecA - B0034 - SYFP2

Participant: Sabri, Lisa
Experiment: Screening, phosphorylation of primers, PCR purification, DPN1 treatment
pEL3S15 - J23101 - random sRNA - Spot42
Result: 10% and 14% DMSO showed bands on the gel

Participant: Fredrik
Experiment: Plasmid prep.
115. pSB3T5 - J23110 - tetA
146. pSB1C3 - T0

Participant: Tobias, Christoffer
Experiment: Screening
cat-sacB
Result: Contamination, no clear bands

Participant: Johan
Experiment: Assembly
33. pSB1A3 - PT5lac - B0034
102. pSB3K5 - J23110 - B0034 - SYFP2 - B1002
172. pSB3C5 - tet inv. - B0034 - SYFP2

Participant: Karl
Experiment: Cloning
97. pSB3T5 - pBad
156. pJN3C1 - AmpR

Participant: Olle
Experiment: Glycerol stock and plasmid prep.
159. pSB1C3 - PLlacO - B0034
160. pSB1C3 - T5lac - B0034
161. pSB1C3 - PrecA - B0034 - SYFP2

Participant: Julia, Hampus
Experiment: Transformation
164. pEL3S15 - Pxis - B0034 - SYFP2
165. ImmR - TλT1

Experiment: O/N
52. pSB1C3 - TλT1

Participant: Joakim, Fredrik
Experiment: Phosphorylation of primers
LexA(D85G)_R
LexA(D85G)_F
marR(mut)_R
marR(mut)_F

Wednesday 25 July

Participant: Emy, Caroline, Katarina, Ellen
Experiment: Screening
118. pEL3A18 - PlacIQ - CTX-M - SYFP2
134. pSB1A3 - J23116 - tetA
162. pEL3A18 - J23110 - AAC(6') - SYFP2
163. pEL3A18 - J23110 - CTX-M - SYFP2
Result: 134 good, 118 and 162 have to be redone. 163 will not be continued.

Participant: Hampus, Julia
Experiment: PCR purification, ligation
pEL3S15 - J23101 - random sRNA - Spot42

Experiment: Assembly
164. pEL3S15 - Pxis - B0034 - SYFP2
165. pSB1K3 - ImmR - TλT1

Participant: Karl
Experiment: Restreak
97. pSB3T5 - pBad
156. pJN3C1 - AmpR

Experiment: New cloning and transformation, in case the previous one failed
97. pSB3T5 - pBad

Experiment: Transformation
14. pSB4K5 - lacIs

Experiment: O/N
158. pSB4k5 - red

Participant: Olle
Experiment: Restreak
33. pSB3T5 - PT5lac - B0034
102. pSB3C5 - J23110 - B0034 - SYFP2 - B1002
172. pSB3C5 - tet inv. B0034 - SYFP2

Participant: Tobias, Christoffer
Experiment: PCR cloning and screening
84. pSB3T5 - cat-sacB
Result: Bad gel.

Participant: Anton
Experiment: Preparation for sequencing
60. pSB1A3 - B0032 - SYFP2
79. pSB1C3 - J23106 - B0034
112. pSB1C3 - marR
146. pSB1C3 - T0

Participant: Julia
Experiment: Plasmid prep.
52. pSb1C3 - TλT1

Participant: Arvid, Anton
Experiment: Screening, preparation for sequencing
60. pSB1A3 - B0032 - SYFP2

Thursday 26 July

Participant: Joel
Experiment: Glycerol stock and plasmid prep.
60. pSB1A3 - B0034 - SYFP2
134. pSB1A3 - J23116 - tetA

Participant: Christoffer, Tobias
Experiment: PCR cloning and screening
84. pSB3T5 - cat-sacB
Result: Good bands.

Participant: Caroline, Emy, Katarina
Experiment: Screening
118. pEL3A18 - PlacIQ - CTX-M - SYFP2
162. pEL3A18 - J23110 - AAC(6') - SYFP2
166. pEL3C18 - PlacIQ - AAC(6') - lacI
167. pEL3C18 - PlacIQ - CTX-M - lacI
168. pEL3C18 - PlacIQ - tetA - lacI
169. pEL3C18 - J23106 - AAC(6') - lacI
170. pEL3C18 - J23106 - tetA - lacI
Result: 118 and 162 had good clones.

Experiment: Restreak
117. pEL3A18 - PlacIQ - AAC(6') - SYFP2

Experiment: O/N
118. pEL3A18 - PlacIQ - CTX-M - SYFP2
162. pEL3A18 - J23110 - AAC(6') - SYFP2

Participant: Hampus, Julia
Experiment: Screening
164. pEL3S15 - Pxis - B0034 - SYFP2
165. pSB1K3 - ImmR - TλT1
Result: Both had clones with the right lengths.

Experiment: O/N
156. pJN3C1 - AmpR
164. pEL3S15 - Pxis - B0034 - SYFP2
165. pSB1K3 - ImmR - TλT1

Participant: Karl
Experiment: Screening
97. pSB3T5 - pBad
172. pSB3C5 - tet inv. - B0034 - SYFP2
Result: 172 was good, 97 was not.

Participant: Olle
Experiment: Restreak
33. pSB3T5 - PT5lac - B0034
102. pSB3C5 - J23110 - B0034 - SYFP2 - B1002

Experiment: O/N
158. pSB3K5 - red

Friday 27 July

Participant: Tobias, Christoffer
Experiment: Assembly
79. pSB1C3 - J23106 - B0034
80. pSB1C3 - J23110 - B0034
81. pSB1C3 - J23116 - B0034

Experiment: DPN1 treatment
84. pSB3T5 - cat-sacB

Participant: Caroline, Emy, Katarina, Joel
Experiment: Screening and O/N
117. pEL3A18 - PlacIQ - AAC(6') - SYFP2
Result: Good!

Experiment: Screening
167. pEL3C18 - PlacIQ - PlacIQ - CTX-M - lacI
Result: Bad! Turns out lacI was something completely different, it had been taken from the wrong well in the kit.

Experiment: Assembly
166. pEL3C18 - PlacIQ - AAC(6') - lacI
167. pEL3C18 - PlacIQ - CTX-M - lacI
168. pEL3C18 - PlacIQ - tetA - lacI
169. pEL3C18 - J23106 - AAC(6') - lacI

Experiment: Streak from frozen stock
37. pSB3T5 - PlacIQ - CTX-M
38. pSB3T5 - PlacIQ - tetA
56. pSB3T5 - J23106 - tetA

Participant: ELlen
Experiment: Assembly
173. pEL3C18 - J23106 - CTX-M - SYFP2
174. pEL3C18 - J23106 - CTX-M - lacI

Experiment: Transformation
173. pEL3C18 - J23106 - CTX-M - SYFP2
174. pEL3C18 - J23106 - CTX-M - lacI
166. pEL3C18 - PlacIQ - AAC(6') - lacI
167. pEL3C18 - PlacIQ - CTX-M - lacI
168. pEL3C18 - PlacIQ - tetA - lacI
169. pEL3C18 - J23106 - AAC(6') - lacI

Participant: Olle, Karl
Experiment: Screening
33. pSB3T5 - PT5lac - B0034
97. pSB3T5 - pBad
102. pSB3C5 - J23110 - B0034 - SYFP2 - B1002
Result: All wrong.

Participant: Hampus
Experiment: Assembly and transformation
139. pSB3T5 - PT5lac - B0034 - SYFP2
177. pSB3T5 - AmpR - T0

Experiment: Glycerol stock and plasmid prep.
156. pJN3C1 - AmpR
164. pEL3S15 - Pxis - B0034 - SYFP2
165. pSB1K3 - ImmR - TλT1

Participant: Olle
Experiment: Restreak
101. pSB3T5 - J23110 - B0034 - SYFP2

Participant: Arvid
Experiment: Glycerol stock and plasmid prep.
118. pEL3A18 - PlacIQ - CTX-M - SYFP2
158. pSB4K5 - red
162. pEL3A18 - J23110 - AAC(6') - SYFP2
172. pSB3C5 - tet inv. - B0034 - SYFP2

Saturday 28 July

Participant: Tobias, Christoffer
Experiment: Transformation
79. pSB1C3 - J23106 - B0034
80. pSB1C3 - J23110 - B0034
81. pSB1C3 - J23116 - B0034

Participant: Arvid, Karl
Experiment: Screening
33. pSB3T5 - PT5lac - B0034
97. pSB3T5 - pBad
101. pSB3T5 - J23110 - B0034 - SYFP2
158. pSB4K5 - red
172. pSB3C5 - tet inv. - B0034 - SYFP2
Result: 33 and 97 wrong, 101 contaminated and has to be screened again, 158 and 172 seem OK.

Participant: Caroline, Emy, Katarina
Experiment: Phosphorylation of primers for and mutagenesis of:
178. pEL3C18 - J23116 - AAC(6') - linker - lacIs
179. pEL3C18 - PlacIQ - AAC(6') - linker - lacIs
180. pEL3C18 - J23106 - AAC(6') - linker - SYFP2

Participant: Joel
Experiment: Assembly and transformation
137. pEL3C18 - PlacIQ - CTX-M - lacIs
138. pEL3C18 - PlacIQ - tetA - lacIs
151. pEL3C18 - J23106 - AAC(6') - lacIs
182. pEL3C18 - J23106 - CTX-M - lacIs
183. pSB1C3 - Ptac

Participant: Karl
Experiment: O/N
37. pSB3T5 - PlacIQ - CTX-M
38. pSB3T5 - PlacIQ - tetA
56. pSB3T5 - J23106 - tetA
101. pSB3T5 - J23116 - B0034 - SYFP2 - B1002
155. pSB1A2 - lacI

Participant: Fredrik
Experiment: Assembly
175. pSB1K3 - PLlacO - B0034
176. pSB1K3 - PT5lacO - B0034

Participant: Ellen
Experiment: Glycerol stock and plasmid prep.
33. pSB3T5 - PT5lac - B0034
117. pEL3A18 - PlacIQ - AAC(6') - SYFP2

Participant: Hampus
Experiment: Restreak
139. pSB3T5 - PT5lac - B0034 - SYFP2
177. pSB3T5 - AmpR - T0

Sunday 29 July

Participant: Hampus
Experiment: Ligation
92. pEL3S15 - J23101 - random sRNA - Spot42

Participant: Tobias, Christoffer
Experiment: Restreak
79. pSB1C3 - J23106 - B0034
80. pSB1C3 - J23110 - B0034
81. pSB1C3 - J23116 - B0034

Participant: Karl
Experiment: Streak from frozen stock
60. pSB1A3 - B0032 - SYFP2

Participant: Olle
Experiment: Screening
101. pSB3T5 - J23110 - B0034 - SYFP2
Result: Possibly one good clone

Participant: Fredrik, Pikkei
Experiment: Mutagenesis PCR
112. pSB1C3 - MarR
147. pSB1C3 - LexA
148. pSB1C3 - OmpF
Result: All had clones of the correct length.

Participant: Caroline, Katarina, Emy, Joel
Experiment: Mutagenesis, transformation
178. pEL3C18 - J23116 - AAC(6') - linker - lacIs
179. pEL3C18 - PlacIQ - AAC(6') - linker - lacIs
180. pEL3A18 - J23106 - AAC(6') - linker - SYFP2

Experiment: Assembly and transformation
152. pEL3C18 - J23106 - tetA - lacIs
181. pEL3A18 - J23106 - CTX-M - SYFP2
Result: All clones of 152 were red, assembly will be redone.

Experiment: Restreak
137. pEL3C18 - PlacIQ - CTX-M - lacIs
138. pEL3C18 - PlacIQ - tetA - lacIs
151. pEL3C18 - J23106 - AAC(6') - lacIs
182. pEL3C18 - J23106 - CTX-M - lacIs
183. pSB1C3 - Ptac

Participant: Hampus
Experiment: Restreak
139. pSB3T5 - PT5lac - B0034 - SYFP2
177. pSB3T5 - AmpR - TT0

Participant: Ellen
Experiment: Glycerol stock and plasmid prep.
37. pSB3T5 -PlacIQ - CTX-M
38. pSB3T5 - PlacIQ - tetA
56. pSB3T5 - J23106 - tetA
101. pSB3T5 - J23110 - B0034 - SYFP2 - B1002
155. pSB1A2 - lacI

Experiment: Streak from frozen stock
56. pSB3T5 - J23106 - tetA

Monday 30 July

Participant: Julia, Hampus
Experiment: Screening
79. pSB1C3 - J23106 - B0034
80. pSB1C3 - J23110 - B0034
81. pSB1C3 - J23116 - B0034
139. pSB3T5 - PT5lac - B0034 - SYFP2
177. pSB3T5 - AmpR - TT0
183. pSB1C3 - Ptac
Result: 79, 80, 81, 183 gave good bands. 139 and 177 showed the wrong bands.

Experiment: O/N
79. pSB1C3 - J23106 - B0034
80. pSB1C3 - J23110 - B0034
81. pSB1C3 - J23116 - B0034
183. pSB1C3 - Ptac

Participant: Anton
Experiment: Assembly and transformation
184. pEL3S15 - AmpR - T0

Participant: Tobias, Christoffer
Experiment: Insertion of cat-sacB onto the chromosome of MG1655-J23101 with λ-red

Participant: Caroline, Emy, Katarina
Experiment: Screening
137. pEL3C18 - PlacIQ - CTX-M - lacIs
138. pEL3C18 - PlacIQ - tetA - lacIs
151. pEL3C18 - J23106 - AAC(6') - lacIs
182. pEL3C18 - J23106 - CTX-M - lacIs
Result: 151 and 182 OK, 137 and 138 failed.

Experiment: O/N
56. pSB3T5 - J23106 - tetA
60. pSB1A3 - B0032 - SYFP2
151. pEL3C18 - J23106 - AAC(6') - lacIs
182. pEL3C18 - J23106 - CTX-M - lacIs

Experiment: Restreak
178. pEL3C18 - J23116 - AAC(6') - linker - lacIs
179. pEL3C18 - PlacIQ - AAC(6') - linker - lacIs
180. pEL3A18 - J23106 - AAC(6') - linker - SYFP2
181. pEL3A18 - J23106 - CTX-M - SYFP2

Participant: Olle
Experiment: Phosphorylation of primers
pUC_BB_F
pUC_BB_R
pEL8to4C15_F
pEL8to4C15_R

Experiment: Phusion PCR to BioBrick pUC ORI from pSB1A3

Participant: Fredrik
Experiment: PCR purification, ligation and transformation
112. pSB1C3 - MarR
147. pSB1C3 - LexA
148. pSB1C3 - OmpF

Experiment: Screening
175. pSB1K3 - PLlacO - B0034
Result: All clones wrong.

Tuesday 31 July

Participant: Arvid
Experiment: Prepare agar stabs for sending to other teams
pSB1A3 - amilCP
pSB1C3 - amilGFP
pSB1C3 - FixJ
pSB1C3 - YF1
pSB1C3 - B0034 - YF1 - B0034 - FixJ
pSB1C3 - PfixK2

Participant: Joakim
Experiment: Restreak
184. pEL3S15 - AmpR - T0

Participant: Karl
Experiment: O/N
12. pSB1A2 - B0034

Experiment: PCR purification
BioBricked pUC-ORI
182. pEL3C18 - J23106 - CTX-M - lacIs

Experiment: Mutagenesis PCR of pEL8C15 to pEL4C15

Experiment: Screening
pUC-ORI
pEL4C15
Result: Both seem OK.

Participant: Emy, Caroline, Katarina, Ellen
Experiment: Screening
137. pEL3C18 - PlacIQ - CTX-M - lacIs
138. pEL3C18 - PlacIQ - tetA - lacIs
181. pEL3A18 - J23106 - CTX-M - SYFP2
Result: 137 and 138 have clones of the correct lengths. 181 was all wrong.

Experiment: Assembly and transformation
152. pEL3C18 - J23106 - tetA
181. pEL3A18 - J23106 - CTX-M - SYFP2

Participant: Joel, Karl
Experiment: Screening
14. pSB4K5 - lacIs
178. pEL3C18 - J23116 - AAC(6') - linker - lacIs
179. pEL3C18 - PlacIQ - AAC(6´) - linker - lacIs
180. pEL3A18 - J23106 - AAC(6') - linker - SYFP2
Result: All seem to have good clones.

Experiment: O/N
137. pEL3C18 - PlacIQ - CTX-M - lacIs
138. pEL3C18 - PlacIQ - tetA - lacIs

Participant: Olle
Experiment: Digestion
BioBricked pUC-ORI

Participant: Hampus, Julia
Experiment: Screening and O/N
139. pSB3T5 - PT5lac - B0034 - SYFP2
177. pSB3T5 - AmpR - TT0
Result: Possible good clones of both.

Experiment: Assembly
186. pJN3K1 - AmpR - TT0

Participant: Fredrik
Experiment: Plasmid prep.
1. pSB1C3 - red
12. pSB1A2 - B0034
86. pSB1A2 - PLlacO

Experiment: Assembly
175. pSB1K3 - PLlacO - B0034

Participant: Julia
Experiment: Plasmid prep.
60. pSB1A3 - B0032 - SYFP2
79. pSB1C3 - J23106 - B0034
80. pSB1C3 - J23110 - B0034
81. pSB1C3 - J23116 - B0034
183. pSB1C3 - Ptac

Participant: Christoffer, Tobias
Experiment: Streak MG1655 - J23101 - cat-sacB on plates with chloramphenicol and sucrose.

Experiment: NotI treatment
79. pSB1C3 - J23106 - B0034
80. pSB1C3 - J23110 - B0034
81. pSB1C3 - J23116 - B0034
183. pSB1C3 - Ptac

Participant: Anton, Olle
Experiment: Plasmid prep.
56. pSB3T5 - J23106 - tetA
74. pEL3C15 - PlacIQ - CTX-M - lacIs - TλT1
80. pSB1C3 - J23110 - B0034

Experiment: Preparation for sequencing
151. pEL3C18 - J23106 - AAC(6') - lacIs
182. pEL3C18 - J23106 - CTX-M - lacIs
183. pSB1C3 - Ptac

Wednesday 1 August

Participant: Caroline, Emy, Katarina
Experiment: Screening
138. pEL3C18 - tetA - lacIs
Result: One good clone!

Experiment: Glycerol stock and plasmid prep.
post-mutagenesis pSB4K5 - lacIs
137. pEL3C18 - PLlacO - CTX-M - lacIs
138. pEL3C18 - tetA - lacIs
179. pEL3C18 - PlacIQ - AAC(6') - linker - lacIs
180. pEL3A18 - PlacIQ - AAC(6') - linker - SYFP2

Experiment: Restreak
152. pEL3A18 - J23106 - tetA - lacIs
178. pEL3C18 - J23116 - AAC(6') - linker - lacIs
180. pEL3A18 - J23106 - AAC(6') - linker - SYFP2

Participant: Joel
Experiment: Plasmid prep.
12. pSB1A2 - B0034

Participant: Fredrik
Experiment: Screening and O/N
190. pSB1C3 - MarR(mut)
191. pSB1C3 - LexA(G85D)
192. pSB1C3 - OmpF
Result: All had good clones!

Experiment: Transformation
175. pSB1C3 - PLlacO - B0034

Participant: Joakim, Johan
Experiment: Screening
184. pEL3S15 - AmpR - T0
Result: Contamination. Construct discontinued, different backbone will be used.

Participant: Hampus
Experiment: Assembly
139. Plac - B0034 - SYFP2

Participant: Julia, Hampus
Experiment: Restreak
139. pSB3T5 - PT5lac - B0034 - SYFP2
Result: Success!

Participant: Olle, Karl
Experiment: PCR purification
pUC-ORI
pEL8to4C15
1. pSB1C3 - red

Experiment: Ligation
pEL8to4C15
pSB1C3 - pUC - red

Participant: Tobias, Christoffer
Experiment: Restreak
MG1655 - SpecR - J23101 - cat-sacB

Experiment: Screening on acrylamide gel on plasmids digested with NotI
79. pSB1C3 - J23106 - B0034
80. pSB1C3 - J23110 - B0034
81. pSB1C3 - J23116 - B0034
183. pSB1C3 - Ptac

Thursday 2 August

Participant: Fredrik
Experiment: Glycerol stock and plasmid prep.
190. pSB1C3 - MarR(mut)
191. pSB1C3 - LexA(G85D)
192. pSB1C3 - OmpF

Participant: Joel
Experiment: Plasmid prep.
12. pSB1A2 - B0034
127. pEL3A18(FRT) - red

Experiment: Screening and O/N
138. pEL3C18 - PlacIQ - tetA - lacIs
Result: Good!

Participant: Christoffer
Experiment: Screening
196. MG1655 - SpecR - J23101 - cat-sacB
Result: Good!

Participant: Hampus
Experiment: Assembly and transformation
193. pEL3S17 - plac - B0034 - SYFP2 - AmpR - TT0
194. pEL3S17 - PT5lac - B0034 - SYFP2 - AmpR - TT0
195. pSB3T5 - Pxis - B0034 - SYFP2

Experiment: Glycerol stock and plasmid prep.
186. pJN3K1 - AmpR - TT0

Participant: Karl
Experiment: Restreak
pSB1C3 - pUC - red
pEL4C15

Participant: Joel, Caroline, Emy
Experiment: Screening and O/N
152. pEL3C18 - J23106 - tetA - lacIs
178. pEL3C18 - J23116 - AAC(6') - linker - lacIs
181. pEL3A18 - J23106 - CTX-M - SYFP2
Result: Good clones of all contructs!

Experiment: Restreak
181. pEL3A18 - J23106 - CTX-M - SYFP2

Participant: Julia
Experiment: Screening
139. pSB3T5 - PT5lac - B0034 - SYFP2
Result: No clones seem to be right.

Experiment: Assembly and transformation
79. pSB1C3 - J23106 - B0034
80. pSB1C3 - J23110 - B0034
81. pSB1C3 - J23116 - B0034

Friday 3 August

Participant: Karl, Arvid
Experiment: O/N
187. pSB1C3 - pUC - red
188. pEL4C15 - red

Participant: Julia
Experiment: Assembly and transformation
79. pSB1C3 - J23106 - B0034
80. pSB1C3 - J23110 - B0034
81. pSB1C3 - J23116 - B0034

Participant: Katarina, Caroline, Emy
Experiment: Plasmid prep.
152. pEL3A18 - PlacIQ - tetA - SYFP2
178. pEL3C18 - J23116 - AAC(6') - linker - lacIs
181. pEL3A18 - J23106 - CTX-M - SYFP2

Experiment: Screening
181. pEL3A18 - J23106 - CTX-M - SYFP2
Result: All bad.

Experiment: Transformation
181. pEL3A18 - J23106 - CTX-M - SYFP2

Experiment: Preparation for sequencing
119. pEL3A18 - PlacIQ - tetA - SYFP2
138. pEL3C18 - PlacIQ - tetA - lacIs
152. pEL3C18 - J23106 - tetA - lacIs
178. pEL3C18 - J23116 - AAC(6') - linker - lacIs
181. pEL3A18 - J23106 - CTX-M - SYFP2

Participant: Olle
Experiment: Streak from frozen
123. pEL3A18 - red
124. pEL3C18 - red
125. pEL3K18 - red
126. pEL3S18 - red

Participant: Christoffer
Experiment: Glycerol stock
196. MG1655 - SpecR - J23101 - cat-sacB

Experiment: O/N
198. MG1655 - SpecR - J23101 - cat-sacB (without λ-tet-plasmid)

Experiment: Streak 198 on sucrose and tetracycline to make sure it works as it is supposed to

Participant: Ellen
Experiment: Assembly
181. pEL3A18 - J23106 - CTX-M - SYFP2

Participant: Hampus
Experiment: Restreak
193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0
194. pEL3S17 - PT5lac - B0034 - SYFP2 - AmpR - TT0
195. pSB3T5 - Pxis - B0034 - SYFP2

Saturday 4 August

Participant: Hampus
Experiment: Screening
193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0
194. pEL3S17 - PT5lac - B0034 - SYFP2 - AmpR - TT0

Participant: Caroline, Katarina
Experiment: Restreak
181. pEL3A18 - J23106 - CTX-M - SYFP2

Participant: Christoffer
Experiment: Glycerol stock
198. MG1655 - SpecR - J23101 - cat-sacB (without λ-Tet-plasmid)

Participant: Arvid, Karl
Experiment: Screening
187. pSB1C3 - pUC - red
Result: Several good clones.

Experiment: Assembly
33. pSB1A3 - PT5lac - B0034

Experiment: Plasmid prep.
188. pEL4C15 - red

Experiment: O/N
187. pSB1C3 - pUC - red

Participant: Olle
Experiment: PCR amplification
AmpR
AmpR(FRT)
CamR
CamR(FRT)
SpecR
SpecR(FRT)
TetR
TetR(FRT)
KanR(FRT)

Experiment: Screening
AmpR
AmpR(FRT)
CamR
CamR(FRT)
SpecR
SpecR(FRT)
Result: All except CamR(FRT) looked good.

Sunday 5 August

Experiment: Glycerol stock and plasmid prep.
193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0

Participant: Arvid
Experiment: Plasmid prep. of 187. pSB1C3 - pUC - red, PCR amplification of the insert, PCR purification, vacuum drying, cloning into pEL4C15 and transformation.

Experiment: Restreak
33. pSB1A3 - PT5lac - B0034

Participant: Olle
Experiment: Screening
tetR
tetR(FRT)
KanR(FRT)
Result: All had good clones.

Experiment: PCR purification and digestion with DpnI, SacI and SalI
AmpR
AmpR(FRT)
CamR
CamR(FRT)
SpecR
SpecR(FRT)
TetR
TetR(FRT)
KanR(FRT)

Participant: Caroline, Katarina
Experiment: Screening and O/N
181. pEL3A18 - J23106 - CTX-M - SYFP2
Result: Two good clones

Monday 6 August

Participant: Hampus, Fredrik
Experiment: Glycerol stock and plasmid prep.
81. pSB1C3 - J23116 - B0034
159. pSB1C3 - PLlacO - B0034

Participant: Ellen, Katarina
Experiment: O/N
55. pSB3T5 - J23106 - CTX-M

Experiment: Plasmid prep.
181. pEL3A18 - J23106 - CTX-M - SYFP2
34. J61002 - J23106
61. J61002 - J23110

Experiment: Preparation for sequencing
181. pEL3A18 - J23106 - CTX-M - SYFP2

Participant: Sabri, Lisa
Experiment: Assembly/cloning and transformation
79. pSB1C3 - J23106 - B0034
80. pSB1C3 - J23110 - B0034
98. pEL3K17 - PT5lac

Participant: Arvid
Experiment: O/N
33. pSB1A3 - PT5lac - B0034

Tuesday 7 August

Participant: Olle
Experiment: PCR amplification for sequencing, PCR purification
199. pEL4C15 - pUC - red

Participant: Anders, Niklas, Josefin
Experiment: Cloning, screening, PCR purification
TetM
Result: Correct length on gel

Participant: Anton
Experiment: Preparation for sequencing
81. pSB1C3 - OmpF(rsr)
159. pSB1C3 - PLlacO - B0034
139. pSB3T5 - PT5lac - B0034 - SYFP2
190. pSB1C3 - MarR(mut)
193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0

Participant: Hampus, Fredrik
Experiment: Screening
12. pSB1A2 - B0034
35. J61002 - J23116
81. pSB1C3 - J23116 - B0034
86. pSB1A2 - PLlacO
159. pSB1C3 - PLlacO - B0034
Result: All right.

Participant: Sabri, Lisa
Experiment: Assembly and transformation
79. pSB1C3 - J23106 - B0034
80. pSB1C3 - J23110 - B0034

Experiment: Restreak
98. pEL3K17 - PT5lac

Wednesday 8 August

Participant: Lisa, Sabri
Experiment: Screening
79. pSB1C3 - J23106 - B0034
80. pSB1C3 - J23110 - B0034
Result: Expected bands at 426bp, faint bands at around 400bp were observed but disappeared before a picture was taken of the gel. New gel will be run tomorrow.

Experiment: Restreak
98. pEL3K17 - PT5lac

Participant: Olle
Experiment: Screening
33. pSB1A3 - PT5lac -B0034
28. pSB1C3 - PT5lac (used as reference)
Result: 33 is incorrect, only contains the RBS.

Experiment: O/N
199. pEL4C15 - pUC - red

Participant: Josefin, Niklas
Experiment: Cloning
pSB1C3 - tetM

Thursday 9 August

Participant: Anna
Experiment: O/N
79. pSB1C3 - J23106 - B0034
80. pSB1C3 - J23110 - B0034

Experiment: Plasmid prep.
199. pEL4C15 - pUC - red

Participant: Joakim
Experiment: Assembly and transformation
pSB1C3 - J23106 - B0034

Participant: Sabri
Experiment: Screening
98. pEL3K17 - PT5lac
Result: Has to be redone.

Participant: Olle
Experiment: Digestion with SacI and SalI
199. pEL4C15 - pUC - red

Experiment: O/N
28. pSB1C3 - PT5lac

Experiment: Assembly
101. pSB3T5 - J23110 - B0034 - SYFP2

Participant: Sabri, Joel, Katarina
Experiment: Mutagenesis
213. pEL3A18 - J23110 - AAC(6') - linker - SYFP2
214. pEL3A18 - PlacIQ - CTX-M - linker - SYFP2
215. pEL3A18 - PlacIQ - AAC(6') - linker - SYFP2
216. pEL3A18 - PlacIQ - tetA - linker - SYFP2
217. pEL3A18 - J23106 - tetA - linker - SYFP2
218. pEL3A18 - J23106 - tetA - linker - SYFP2
219. pEL3C18 - J23106 - tetA - linker - lacIs
220. pEL3C18 - J23106 - AAC(6') - linker - lacIs
221. pEL3C18 - PlacIQ - CTX-M - linker - lacIs
222. pEL3C18 - PlacIQ - tetA - linker - lacIs

Friday 10 August

Participant: Hampus, Joel
Experiment: Screening
197. pEL3S17 - red
Result: Wrong bands.

Experiment: PCR amplification
pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0

Participant: Hampus
Experiment: Assembly and transformation
164. pSB3T5 - Pxis - B0034 - SYFP2
223. pSB3T5 - J23116 - B0034 - ImmR - TλT1

Participant: Joakim
Experiment: Restreak
79. pSB1C3 - J23106 - B0034

Participant: Sabri, Lisa
Experiment: PCR purification, ligation and transformation
213. pEL3A18 - J23110 - AAC(6') - linker - SYFP2
214. pEL3A18 - PlacIQ - CTX-M - linker - SYFP2
215. pEL3A18 - PlacIQ - AAC(6') - linker - SYFP2
216. pEL3A18 - PlacIQ - tetA - linker - SYFP2
217. pEL3A18 - J23106 - tetA - linker - SYFP2
218. pEL3A18 - J23106 - tetA - linker - SYFP2
219. pEL3C18 - J23106 - tetA - linker - lacIs
220. pEL3C18 - J23106 - AAC(6') - linker - lacIs
221. pEL3C18 - PlacIQ - CTX-M - linker - lacIs
222. pEL3C18 - PlacIQ - tetA - linker - lacIs

Participant: Olle
Experiment: Screening
199. pEL4C15 - pUC - red (cut with SacI and SalI)
Result: Good!

Experiment: Assembly and transformation
230. pEL4A15 - pUC - red
231. pEL4K15 - pUC - red
232. pEL4S15 - pUC - red
233. pEL4A15(FRT) - pUC - red
234. pEL4C15(FRT) - pUC - red
235. pEL4K15(FRT) - pUC - red
236. pEL4S15(FRT) - pUC - red
237. pEL4T15(FRT) - pUC - red
238. pEL4T15 - pUC - red

Participant: Katarina
Experiment: Cloning and transformation
225. pEL4C15 - J23106 - AAC(6') - linker - SYFP2

Participant: Anna, Fredrik
Experiment: Assembly and transformation
202. pSB1K3 - B0034(mod) - LexA(mut)
203. pSB1K3 - B0034(mod) - OmpF(rsr)
204. pSB1K3 - B0034(mod) - marR(mut)
211. pSB3T5 - PLlacO - B0034 - LexA(mut)

Participant: Anders
Experiment: Assembly and transformation
227. pSB3T5 - J23110 - tet inv. - B0034 - SYFP2

Participant: Niklas, Josefin
Experiment: Screening
pSB1C3 - tetM
Result: Gel failed.

Saturday 11 August

Participant: Olle
Experiment: Plasmid prep.
154. pSB1C3 - PT5lac
240. pEL3S17 - P - R - lacIQ

Experiment: Restreak
230. pEL4A15 - pUC - red
231. pEL4K15 - pUC - red
232. pEL4S15 - pUC - red
233. pEL4A15(FRT) - pUC - red
234. pEL4C15(FRT) - pUC - red
235. pEL4K15(FRT) - pUC - red
236. pEL4S15(FRT) - pUC - red
237. pEL4T15(FRT) - pUC - red
238. pEL4T15 - pUC - red

Participant: Hampus
Experiment: Assembly
193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0
228. pEL3S17 - Plac - B0034 - SYFP2 - AmpR

Experiment: Restreak
165. pSB1K3 - ImmR - TλT1
223. pSB3T5 - J23116 - B0034 - ImmR - TλT1

Participant: Anna, Karl
Experiment: Assembly and transformation
202. pSB1K3 - B0034(mod) - LexA(G58D)
203. pSB1K3 - B0034(mod) - OmpF(rsr)
204. pSB1K3 - B0034(mod) - marR(mut)

Experiment: Restreak
211. pSB3T5 - PLlacO - B0034 - LexA(G58D)

Experiment: O/N
79. pSB1C3 - J23106 - B0034
239. pSB1A3 - J23101 - B0034

Participant: Joel, Katarina
Experiment: Restreak
213. pEL3A18 - J23110 - AAC(6') - linker - SYFP2
214. pEL3A18 - PlacIQ - CTX-M - linker - SYFP2
215. pEL3A18 - PlacIQ - AAC(6') - linker - SYFP2
216. pEL3A18 - PlacIQ - tetA - linker - SYFP2
217. pEL3A18 - J23106 - tetA - linker - SYFP2
218. pEL3A18 - J23106 - tetA - linker - SYFP2
219. pEL3C18 - J23106 - tetA - linker - lacIs
220. pEL3C18 - J23106 - AAC(6') - linker - lacIs
221. pEL3C18 - PlacIQ - CTX-M - linker - lacIs
222. pEL3C18 - PlacIQ - tetA - linker - lacIs
225. pEL4C15 - J23106 - AAC(6') - linker - SYFP2

Participant: Olle, Arvid
Experiment: Screening
pBad/AraC amplified from MG1655 chromosome
Result: Good.

Sunday 12 August

Participant: Anders
Experiment: Restreak
226. pSB3T5 - J23106 - tet inv. - B0034 - SYFP2
227. pSB3T5 - J23110 - tet inv. - B0034 - SYFP2

Participant: Joakim
Experiment: Plasmid prep.
79. pSB1A3 - J23101 - B0034
239. pSB1A3 - J23101 - B0034

Experiment: Mutagenesis (addition of B0034 to PT5lac)

Participant: Anna
Experiment: Restreak
202. pSB1K3 - B0034(mod) - LexA(G58D)
203. pSB1K3 - B0034(mod) - OmpF(rsr)
204. pSB1K3 - B0034(mod) - marR(mut)

Experiment: Screening
211. pSB3T5 - PLlacO - B0034 - LexA(G58D)
Result: No correct bands!

Participant: Anders B
Experiment: Screening
BioBricked tetM
Result: Looks good.

Participant: Fredrik
Experiment: Screening and O/N
213. pEL3A18 - J23110 - AAC(6') - linker - SYFP2
216. pEL3A18 - PlacIQ - tetA - linker - SYFP2
217. pEL3A18 - J23106 - CTX-M - linker - SYFP2
225. pEL3C18 - PlacIQ - tetA - linker - SYFP2
Result: All had good clones.

Paticipant: Olle
Experiment: Screening and O/N
214. pEL3A18 - PlacIQ - CTX-M - linker - SYFP2
215. pEL3A18 - PlacIQ - AAC(6') - linker - SYFP2
218. pEL3A18 - J23106 - tetA - linker - SYFP2
Result: All had good clones.

Experiment: Screening
230. pEL4A15 - pUC - red
232. pEL4S15 - pUC - red
233. pEL4A15(FRT) - pUC - red
234. pEL4C15(FRT) - pUC - red
235. pEL4K15(FRT) - pUC - red
236. pEL4S15(FRT) - pUC - red
237. pEL4T15(FRT) - pUC - red
Result: Gel failed, has to be redone.

Participant: Katarina
Experiment: Screening and O/N
219. pEL3C18 - J23106 - tetA - linker - lacIs
220. pEL3C18 - J23106 - AAC(6') - linker - lacIs
221. pEL3C18 - PlacIQ - CTX-M - linker - lacIs
222. pEL3C18 - PlacIQ - tetA - linker - lacIs
Result: All had good clones.

Participant: Hampus
Experiment: Screening
164. pSB3T5 - Pxis - B0034 - SYFP2
223. pSB3T5 - J23116 - ImmR - TλT1
Result: 223 right. One clone of 164 might be right.

Monday 13 August

Participant: Karl, Arvid
Experiment: Screening
101. pSB3T5 - J23110 - B0034 - SYFP2
33. pSB1C3 - PT5lac - B0034
Result: 101 good, 33 has to be screened again.

Participant: Anna, Fredrik
Experiment: Screening
202. pSB1K3 - B0034(mod) - LexA(G58D)
203. pSB1K3 - B0034(mod) - OmpF(rsr)
204. pSB1K3 - B0034(mod) - marR(mut)
Result: All clones of 204 were good, 203 had one good clone, 202 failed.

Participant: Olle
Experiment: Screening
230. pEL4A15 - pUC - red
232. pEL4S15 - pUC - red
233. pEL4A15(FRT) - pUC - red
234. pEL4C15(FRT) - pUC - red
235. pEL4K15(FRT) - pUC - red
236. pEL4S15(FRT) - pUC - red
237. pEL4T15(FRT) - pUC - red
Result: All good!

Experiment: O/N
203. pSB1K3 - B0034(mod) - OmpF(rsr)
230. pEL4A15 - pUC - red
232. pEL4S15 - pUC - red
233. pEL4A15(FRT) - pUC - red
234. pEL4C15(FRT) - pUC - red
235. pEL4K15(FRT) - pUC - red
236. pEL4S15(FRT) - pUC - red
237. pEL4T15(FRT) - pUC - red
238. pEL4T5 - pUC - red

Participant: Anders, Johan
Experiment: Screening
226. pSB3T5 - J23106 - tet inv. - B0034 - SYFP2
227. pSB3T5 - J23110 - tet inv. - B0034 - SYFP2
Result: Contaminated.

Participant. Sabri, Lisa
Experiment: Plasmid prep.
164. pSB3T5 - Pxis - B0034 - SYFP2
220. pEL3C18 - J23106 - AAC(6') - linker - lacIs
221. pEL3C18 - PlacIQ - CTX-M - linker - lacIs
222. pEL3C18 - PlacIQ - tetA - linker - lacIs
223. pSB3T5 - J23116 - B0034 - ImmR - TλT1
225. pEL4C15 - J23106 - AAC(6') - linker - SYFP2

Participant: Joel
Experiment: Glycerol stock and plasmid prep.
241. pEL3S17-laciq - red
242. pEL3K16-laciq - red

Participant: Katarina, Caroline
Experiment: Plasmid prep.
213. pEL3A18 - J23110 - AAC(6') - linker - SYFP2
214. pEL3A18 - PlacIQ - CTX-M - linker - SYFP2
215. pEL3A18 - PlacIQ - AAC(6') - linker - SYFP2
216. pEL3A18 - PlacIQ - tetA - linker - SYFP2
217. pEL3A18 - J23106 - tetA - linker - SYFP2
218. pEL3A18 - J23106 - tetA - linker - SYFP2
219. pEL3C18 - J23106 - tetA - linker - lacIs

Participant: Hampus
Experiment: Screening
193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0
228. pEL3S17 - Plac - B0034 - SYFP2 - AmpR
Result: All bad.

Participant: Anton
Experiment: Preparation for sequncing
164. pSB3T5 - Pxis - B0034 - SYFP2
213. pEL3A18 - J23110 - AAC(6') - linker - SYFP2
214. pEL3A18 - PlacIQ - CTX-M - linker - SYFP2
215. pEL3A18 - PlacIQ - AAC(6') - linker - SYFP2
216. pEL3A18 - PlacIQ - tetA - linker - SYFP2
217. pEL3A18 - J23106 - tetA - linker - SYFP2
218. pEL3A18 - J23106 - tetA - linker - SYFP2
219. pEL3C18 - J23106 - tetA - linker - lacIs
220. pEL3C18 - J23106 - AAC(6') - linker - lacIs
221. pEL3C18 - PlacIQ - CTX-M - linker - lacIs
222. pEL3C18 - PlacIQ - tetA - linker - lacIs
223. pSB3T5 - J23116 - B0034 - ImmR - TλT1
225. pEL4C15 - J23106 - AAC(6') - linker - SYFP2

Tuesday 14 August

Participant: Caroline, Katarina
Experiment: Assembly
248. pEL4C15 - J23110 - CTX-M - SYFP2
249. pEL4C15 - J23110 - tetA - SYFP2
250. pEL3C18 - J23116 - CTX-M - lacIs
251. pEL3C18 - J23116 - tetA - lacIs

Participant: Hampus
Experiment: BioBrick PCR, PCR purification and screening
AmpR
Result: Gave bands of the right length.

Experiment: Assembly (using newly BioBricked AmpR)
228. pEL3S17 - Plac - B0034 - SYFP2 - AmpR
229. pSB3K3 - Plac - B0034 - SYFP2 - AmpR

Participant: Johan
Experiment: Assembly and transformation
202. pSB1K3 - B0034 - LexA(G85D)

Participant: Arvid
Experiment: Screening
33. pSB1C3 - B0034 - PT5lac
Result: Very good!

Participant: Lisa
Experiment: O/N
241. pEL3S17iq - red

Participant: Anton
Experiment: Preparation for sequencing
33. pSB1C3 - PT5lac B0034
79. pSB1C3 - J23106 - B0034
80. pSB1C3 - J23110 - B0034
101. pSB3T5 - J23110 - B0034 - SYFP2
125. pEL3C18 - red
127. pEL3A18(FRT) - red
128. pEL3C18(FRT) - red
129. pEL3K18(FRT) - red
182. pEL3C18 - J23106 - CTX-M - lacIs
203. pSB1K3 - B0034(mod) - ompF(rsr)
204. pSB1K3 - B0034(mod) - marR(mut)
223. pSB3T5 - J23116 - B0034 - ImmR - TλT1
224. pSB1C3 - tetM

Participant: Caroline, Katarina
Experiment: Plasmid prep.
230. pEL4A15 - pUC - red
232. pEL4S15 - pUC - red
233. pEL4A15(FRT) - pUC - red
234. pEL4C15(FRT) - pUC - red
235. pEL4K15(FRT) - pUC - red
236. pEL4S15(FRT) - pUC - red
237. pEL4T15(FRT) - pUC - red

Participant: Joel
Experiment: Promoter exchange PCR (and screening)
Plac -> CP6
Result: PCR failed.

Participant: Sabri, Lisa
Experiment: Plasmid prep.
53. pSB3T5 - Plac - B0034 - SYFP2
182. pEL3C18 - J23106 - CTX-M - lacIs
204. pSB1K3 - B0034(mod) - marR(mut)
223. pSB3T5 - J23116 - B0034 - ImmR - TλT1
236. pEL4S15(FRT) - pUC - red
238. pEL4T15 - pUC - red
241. pEL3S17iq - red
242. pEL3K16iq - red

Participant: Anders
Experiment: Screening
226. pSB3T5 - J23106 - tet inv. - B0034 - SYFP2
227. pSB3T5 - J23110 - tet inv. - B0034 - SYFP2
Result: Extra bands, different PCR protocol will be used.

Participant: Joakim
Experiment: Cloning and transformation
pEL3S17 - Plac - b0034 - SYFP2

Wednesday 15 August

Participant: Olle
Experiment: Preparation for sequencing
230. pEL4A15 - pUC - red
232. pEL4S15 - pUC - red
233. pEL4A15(FRT) - pUC - red
234. pEL4C15(FRT) - pUC - red
235. pEL4K15(FRT) - pUC - red
236. pEL4S15(FRT) - pUC - red
237. pEL4T15(FRT) - pUC - red
238. pEL4T15 - pUC - red

Experiment: Digestion with E+P
pBad

Participant: Joakim
Experiment: Assembly
206. pSB3T5 - J23101 - B0034
208. pSB3T5 - J23101 - B0034 - marR(mut)

Experiment: Transformation
211. pSB3T5 - PLlacO - B0034 - LexA(G85D)

Participant: Anton
Experiment: Restreak
246. pEL3K18 - J23106 - B0034 - TetM
247. pEL3K18 - J23110 - B0034 - TetM
pEL3S17 - R - lacIQ

Participant: Anders
Experiment: Screening
226. pSB3T5 - J23106 - tet inv. - B0034 - SYFP2
227. pSB3T5 - J23110 - tet inv. - B0034 - SYFP2
Result: Gel failed.

Participant: Sabri
Experiment: Transformation
98. pEL3K17 - PT5lac
228. pEL3S18 - Plac - B0034 - SYFP2 - AmpR
229. pSB3K3 - Plac - B0034 - SYFP2 - AmpR
248. pEL4C15 - J23110 - CTX-M - SYFP2
249. pEL4C15 - J23110 - tetA - SYFP2
250. pEL3C18 - J23116 - CTX-M - lacIs
251. pEL3C18 - J23116 - tetA - lacIs

Participant: Caroline, Katarina
Experiment: Plasmid prep.
33. pSB1C3 - PT5lac - B0034
101. pSB3T5 - J23110 - B0034 - SYFP2

Participant: Arvid
Experiment: O/N from frozen stock
123. pEL3A18 - red
124. pEL3C15 - red
125. pEL3K18 - red
126. pEL3S18 - red
127. pEL3A18(FRT) - red
128. pEL3C18(FRT) - red
129. pEL3K18(FRT) - red
130. pEL3S18(FRT) - red

Participant: Fredrik
Experiment: Screening and O/N
202. pSB1K3 - B0034(mod) - LexA(G85D)
203. pSB1K3 - B0034(mod) - OmpF(rsr)
Result: Both had good clones.

Participant: Anna, Fredrik
Experiment: Plasmid prep.
202. pSB1K3 - B0034(mod) - LexA(G85D)
203. pSB1K3 - B0034(mod) - OmpF

Thursday 16 August

Participant: Caroline, Katarina
Experiment: Plasmid prep.
123. pEL3A18 - red
124. pEL3C15 - red
125. pEL3K18 - red
126. pEL3S18 - red
128. pEL3C18(FRT) - red
129. pEL3K18(FRT) - red
130. pEL3S18(FRT) - red

Participant: Fredrik, Anna, Joakim
Experiment: O/N
252. MG1655 (Acceptor Cipro.R)
253. R1drd19 - C.R
254. R46 - T.R
255. pSa - K.R
256. N3T - T.R

Participant: Joakim
Experiment: Transformation
206. pSB3T5 - J23101 - B0034 - OmpF(rsr)
208. pSB3T5 - J23101 - B0034 - marR(mut)

Experiment: Cloning
245. pEL3S17 - Plac - B0034 - SYFP2
262. pEL3K16 - PT5lac - B0034

Experiment: Restreak
245. pEL3S17 - Plac - B0034 - SYFP2

Participant: Joel
Experiment: Digest and screening
60. pSB1A3 - B0032 - SYFP2
Result: No good clones.

Experiment: Mutagenesis PCR, screening, PCR purification, DPNI
260. pEL3C18 - J23106 - CTX-M(5'UTR) - lacIs
Result: Good!

Participant: Arvid
Experiment: Assembly
102. pSB3K5 - J23110 - B0034 - SYFP2 B1002

Experiment: PCR for insertion of lacIq
pEL4x15

Friday 17 August

Participant: Anna, Fredrik, Joakim, Karl
Experiment: Test of conjugation
253. R1drd19
254. R46
255. pSA
256. N3T

Participant: Caroline, Katarina
Experiment: Screening
248. pEL4C15 - J23110 - CTX-M - SYFP2
249. pEL4C15 - J23110 - tetA - SYFP2
250. pEL3C18 - J23116 - CTX-M - lacIs
251. pEL3C18 - J23116 - tetA - lacIs
Result: One clone of 250 was right, the others were all wrong.

Experiment: PCR purification
260. pEL3C18 - J23106 - CTX-M - linker - lacIs

Experiment: Transformation
60. pSB1A3 - B0032 - SYFP2
260. pEL3C18 - J23106 - CTX-M - linker - lacIs

Participant: Joakim
Experiment: Restreak
206. pSB3T5 - J23101 - B0034 - OmpF(rsr)
208. pSB3T5 - J23101 - B0034 - marR(mut)

Experiment: Transformation
245. pEL3S17 - Plac - B0034 - SYFP2
262. pEL3K16 - PT5lac - B0034

Participant: Sabri, Lisa
Experiment: Making competent MG1655 cells

Experiment: Transformation
79. pSB1C3 - J23106 - B0034
225. pEL4C15 - J23106 - AAC(6') - linker - SYFP2 (in MG1655)

Participant: Arvid
Experiment: Transformation
102. pSB3C5 - J23110 - B0034 - SYFP2

Participant: Hampus
Experiment: Screening and restreak
228. pEL3X17 - Plac - B0034 - SYFP2 - AmpR
229. pSB3K3 - Plac - B0034 - SYFP2 - AmpR

Experiment: Assembly
79. pSB3T5 - J23106 - B0034
164. pSB3T5 - Pxis - B0034 - SYFP2
Result: The parts 164 was assembled from turned out to be wrong.

Saturday 18 August

Participant: Joakim
Experiment: Restreak
245. pEL3S17 - Plac - B0034 - SYFP2
262. pEL3K16 - PT5lac - B0034

Experiment: Screening
245. pEL3S17 - Plac - B0034 - SYFP2
262. pEL3K16 - PT5lac - B0034
Result: Screening has to be redone.

Participant: Hampus
Experiment: Transformation
225. pEL4C15 - J23106 - AAC(6') - linker - SYFP2 (in MG1655)

Experiment: Restreak
79. pSB3T5 - J23106 - B0034

Participant: Hampus, Karl
Experiment: Screening
87. pSB1C3 - pBad
228. pEL3S17 - Plac - B0034 - SYFP2 - AmpR
Result: 87 had no good clones, 228 has to be screened again due to contamination.

Participant: Karl
Experiment: O/N
87. pSB1C3 - pBad
pGEM - eforRed
pGEM - aeBlue
pGEM - cjBlue

Participant: Anna
Experiment: Screening and O/N
206. pSB3T5 - J23101 - B0034 - OmpF(rsr)
208. pSB3T5 - J23101 - B0034 - marR(mut)
211. pSB3T5 - PLlacO - B0034 - LexA(G85D)

Participant: Katarina, Caroline, Emy
Experiment: Screening
250. pEL3C18 - J23116 - CTX-M - lacIs
257. pEL4C15 - J23110 AAC(6') - linker - SYFP2
258. pEL4C15 - PlacIQ - CTX-M - linker - SYFP2
259. pEL4C15 - J23106 - CTX-M - linker - SYFP2
Result: All had good clones.

Experiment: O/N
257. pEL4C15 - J23110 AAC(6') - linker - SYFP2
258. pEL4C15 - PlacIQ - CTX-M - linker - SYFP2
259. pEL4C15 - J23106 - CTX-M - linker - SYFP2

Experiment: Glycerol stock and plasmid prep.
250. pEL3C18 - J23116 - CTX-M - lacIs

Experiment: Restreak
60. pSB1A3 - B0032 - SYFP2
250. pEL3C18 - J23116 - CTX-M - lacIs
260. pEL3C18 - J23106 - CTX-M - linker - lacIs

Experiment: Assembly and transformation
248. pEL4C15 - J23110 - CTX-M - SYFP2
249. pEL4C15 - J23110 - tetA - SYFP2
250. pEL3C18 - J23116 - CTX-M - lacIs
251. pEL3C18 - J23116 - tetA - lacIs

Sunday 19 August

Participant: Karl
Experiment: Plasmid prep.
263. pGEM - eforRed
264. pGEM - aeBlue
265. pGEM - cjBlue

Experiment: Cloning from PCR product
87. pSB1C3 - pBad/AraC

Participant: Caroline, Katarina
Experiment: Screening
60. pSB1A3 - B0032 - SYFP2
250. pEL3C18 - J23116 - CTX-M - lacIs
260. pEL3C18 - J23106 - CTX-M - linker - lacIs

Experiment: Plasmid prep.
257. pEL4C15 - J23110 - AAC(6') - linker - SYFP2
258. pEL4C15 - PlacIQ - CTX-M - linker - SYFP2
259. pEL4C15 - J23106 - CTX-M - linker - SYFP2

Experiment: Transformation
248. pEL4C15 - J23110 - CTX-M - SYFP2
249. pEL4C15 - J23110 - tetA - SYFP2
250. pEL3C18 - J23116 - CTX-M - lacIs
251. pEL3C18 - J23116 - tetA - lacIs

Experiment: O/N
60. pSB1A3 - B0032 - SYFP2
228. pEL3S17 - Plac - B0034 - SYFP2 - AmpR
250. pEL3C18 - J23116 - CTX-M - lacIs
260. pEL3C18 - J23106 - CTX-M - linker - lacIs

Monday 20 August

Participant: Caroline, Katarina
Experiment: Plasmid prep.
60. pSB1A3 - B0032 - SYFP2
228. åEL3S17 - Plac - B0034 - SYFP2 - AmpR
250. pEL3C18 - J23116 - CTX-M - lacIs
260. pEL3C18 - J23106 - CTX-M - linker - lacIs

Experiment: O/N
60. pSB1A3 - B0032 - SYFP2

Tuesday 21 August

Participant: Anna, Fredrik
Experiment: Assembly and transformation
204. pSB1K3 - B0034(mod) - marR(mut)
206. pSB3T5 - J23101 - B0034 - OmpF(rsr)
207. pSB3T5 - J23110 - B0034 - OmpF(rsr)
208. pSB3T5 - J23101 - B0034 - marR(mut)
211. pSB3T5 - PLlacO - B0034 - LexA(G85D)
261. pSB3K3 - PT5lac - B0034 - LexA(G85D)

Experiment: O/N
152. pEL3C18 - J23106 - tetA - lacIs
153. pJN3C1 - Red
154. pSB1C3 - PT5lac
155. pSB1A2 - lacI
156. pJN3C1 - AmpR

Participant: Karl
Experiment: Assembly and transformation
266. pSB1C3 - eforRed
267. pSB1C3 - aeBlue
268. pSB1C3 - cjBlue

Participant: Arvid
Experiment: Mutagenesis
pEL3K16 - redq (from red)
pEL8(A-T)17(FRT) - redq

Experiment: Mutagenesis
pEL3K16iq - red -> redq
pEL4[A-T]17(FRT) - red -> redq
Result: No band on gel.

Participant: Caroline, Katarina, Emy
Experiment: Plasmid prep.
228. pEL3S17 - Plac - B0034 - SYFP2 - AmpR

Experiment: O/N
58. pSB3T5 - J23116 - CTX-M
114. pSB3T5 - J23110 - CTX-M
134. pSB1A3 - J23116 - tetA
189. pSB4K5 - lacIs

Experiment: Digestion with notI
12. pSB1A2 - B0034
79. pSB1C3 - J23106 - B0034

Experiment: Streak from frozen stock
3. pSB1C3 - red
39. pSB1K3 - B0034 - SYFP2
58. pSB3T5 - J23116 - CTX-M
114. pSB3T5 - J23110 - CTX-M
132. pSB1C3 - Pxis
134. pSB1A3 - J23116 - tetA
178. pEL3C18 - J23116 - AAC(6') - linker - lacIs
179. pEL3C18 - PlacIQ - AAC(6')- linker - lacIs
189. pSB4K5 - lacIs
219. pEL3C18 - J23106 - tetA - linker - lacIs
220. pEL3C18 - J23106 - AAC(6') - linker - lacIs
221. pEL3C18 - PlacIQ - CTX-M - linker - lacIs
222. pEL3C18 - PlacIQ - tetA - linker - lacIs
225. pEL4C15 - J23106 - AAC(6') - linker - SYFP2

Participant: Julia, Sabri
Experiment: Transformation
MG1655 225 + sRNA
MG1655 257 + sRNA
Result: Positive controll failed, has to be redone.

Participant: Sabri, Julia
Experiment: Making competent MG1655 cells containing reporter systems 225 (SYFP2) and 257 (lacIs)

Experiment: Transforation of randomized sRNAs into previously made competent cells.

Thursday 23 August

Participant: Joakim
Experiment: Screening
245. pEL3S17 - Plac - B0034 - SYFP2
262. pEL3K16 - PT5lac B0034
Result: No correct band.

Participant: Joel
Experiment: Assembly
301. pSB3T5 - Plac - B0032 - SYFP2
302. pSB3T5 - PlaciQ - B0032 - SYFP2
303. pSB3T5 - PT5lac - B0032 - SYFP2
304. pSB3T5 - PLlacO - B0032 - SYFP2
305. pSB3T5 - J23101 - B0032 - SYFP2
306. pSB3T5 - J23106 - B0032 - SYFP2
307. pSB3T5 - J23110 - B0032 - SYFP2
308. pSB3T5 - J23116 - B0032 - SYFP2
309. pSB3T5 - Ptac - B0032 - SYFP2
310. pSB3T5 - Pxis - B0032 - SYFP2

Participant: Joel, Karl
Experiment: Restreak
251. pEL3C18 - J23116 - tetA - lacIs
300. pEL3C18 - J23116 - CTX-M - linker - lacIs
301. pSB3T5 - Plac - B0032 - SYFP2
302. pSB3T5 - PlaciQ - B0032 - SYFP2
303. pSB3T5 - PT5lac - B0032 - SYFP2
304. pSB3T5 - PLlacO - B0032 - SYFP2
305. pSB3T5 - J23101 - B0032 - SYFP2
306. pSB3T5 - J23106 - B0032 - SYFP2
307. pSB3T5 - J23110 - B0032 - SYFP2
308. pSB3T5 - J23116 - B0032 - SYFP2
309. pSB3T5 - Ptac - B0032 - SYFP2
310. pSB3T5 - Pxis - B0032 - SYFP2
311. pSB3T5 - B0032 - SYFP2
316. pEL3A18 - J23110 - CTX-M - SYFP2
317. pEL3A18 - Plac - B0034 - SYFP2 - AmpR - T0

Participant: Caroline, Katarina
Experiment: Assembly and transformation
248. pEL4C15 - J23110 - CTX-M - SYFP2
249. pEL4C15 - J23110 - tetA - SYFP2
250. pEL3C18 - J23116 - CTX-M - lacIs
251. pEL3C18 - J23116 - tetA - lacIs
311. pSB3T5 - B0032 - SYFP2

Experiment: Plasmid prep.
3. pSB3T5 - red
39. pSB1K3 - B0034 - SYFP2
58. pSB3T5 - J23116 - CTX-M
114. pSB3T5 - J23110 - CTX-M
132. pSB1C3 - Pxis
134. pSB1A3 - J23116 - tetA
189. pSB4K5 - lacIs

Experiment: Mutagenesis
250. pEL3C18 - J23116 - CTX-M - lacIs

Participant: Arvid
Experiment: Mutagenesis
pEL3K16iq - red -> redq
pEL4[A-T]17(FRT) - red -> redq

Participant: Anna, Fredrik
Experiment: Screening and O/N
204. pSB1K3 - B0034(mod) - marR(mut)
206. pSB3T5 - J23101 - B0034 - OmpF(rsr)
207. pSB3T5 - J23110 - B0034 - OmpF(rsr)
208. pSB3T5 - J23101 - B0034 - marR(mut)
211. pSB3T5 - PLlacO - B0034 - LexA(G85D)
261. pSB3K3 - PT5lac - B0034 - LexA(G85D)
Result: 204, 208 and 211 had good clones, 206, 207 and 261 did not.

Participant: Julia
Experiment: Assembly and transformation
164. pSB3T5 - Pxis - B0034 - SYFP2

Friday 24 August

Participant: Pikkei, Karl
Experiment: Cloning
266. pSB1C3 - eforRed
267. pSB1C3 - aeBlue
268. pSB1C3 - cjBlue
320. pSB1C3 - PT5Imm

Participant: Hampus
Experiment: Transformation
MG1655 258
MG1655 259

Experiment: Restreak
164. pSB3T5 - Pxis - B0034 - SYFP2

Experiment: O/N
MG1655 225
MG1655 257

Participant: Joakim
Experiment: Screening
245. pEL3S17 - Plac - B0034 - SYFP2
262. pEL3K16iq - PT5lac - B0034
Result: All wrong.

Participant: Caroline, Katarina
Experiment: PCR purification
300. pEL3C18 - J23116 - CTX-M - linker - lacIs

Experiment: Streak from frozen stock
60. pSB1A3 - B0032 - SYFP2

Experiment: Screening
12. pSB1A2 - B0034
79. pSB3T5 - J23106 - B0034

Experiment: Transforming
300. pEL3C18 - J23116 - CTX-M - linker - lacIs
251. pEL3C18 - J23116 - tetA - lacIs
311. pSB3T5 - B0032 - SYFP2

Participant: Joel
Experiment: Assembly
316. pEL3A18 - J23110 - CTX-M - SYFP2
317. pEL3A18 - J23110 - tetA - SYFP2

Experiment: Transformation
301. pSB3T5 - Plac - B0032 - SYFP2
302. pSB3T5 - PlaciQ - B0032 - SYFP2
303. pSB3T5 - PT5lac - B0032 - SYFP2
304. pSB3T5 - PLlacO - B0032 - SYFP2
305. pSB3T5 - J23101 - B0032 - SYFP2
306. pSB3T5 - J23106 - B0032 - SYFP2
307. pSB3T5 - J23110 - B0032 - SYFP2
308. pSB3T5 - J23116 - B0032 - SYFP2
309. pSB3T5 - Ptac - B0032 - SYFP2
310. pSB3T5 - Pxis - B0032 - SYFP2
316. pEL3A18 - J23110 - CTX-M - SYFP2
317. pEL3A18 - J23110 - tetA - SYFP2

Saturday 25 August

Participant: Hampus, Sabri
Experiment: Screening
165. pSB3T5 - Pxis - B0034 - SYFP2

Experiment: Restreak
193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0
318. pEL3S16 - Plac - B0034 SYFP2 - AmpR - TT0
320. pSB1C3 - PT5Imm

Experiment: Transformation of sRNA into MG1655 containing 257

Participant: Fredrik
Experiment: Assembly and transformation
202. pSB1K3 - B0034(mod) - LexA(G85D)
203. pSB1K3 - B0034(mod) - OmpF(rsr)
206. pSB3T5 - J23101 - B0034 - OmpF(rsr)
207. pSB3Y5 - J23110 - B0034 - OmpF(rsr)
261. pSB3K3 - PT5lac - B0034 - LexA(G85D)

Participant: Caroline, Katarina
Experiment: Transformation
225. pEL4C15 - J23106 - AAC(6') - linker - SYFP2

Participant: Lisa
Experiment: Restreak
MG1655 258 pEL4C15 - PlacIQ - CTX-M - linker - SYFP2
MG1655 259 pEL4C15 - J23106 - CTX-M - linker - SYFP2

Sunday 26 August

Participant: Joel
Experiment: Screening
301. pSB3T5 - Plac - B0032 - SYFP2
302. pSB3T5 - PlaciQ - B0032 - SYFP2
303. pSB3T5 - PT5lac - B0032 - SYFP2
304. pSB3T5 - PLlacO - B0032 - SYFP2
305. pSB3T5 - J23101 - B0032 - SYFP2
306. pSB3T5 - J23106 - B0032 - SYFP2
307. pSB3T5 - J23110 - B0032 - SYFP2
308. pSB3T5 - J23116 - B0032 - SYFP2
309. pSB3T5 - Ptac - B0032 - SYFP2
310. pSB3T5 - Pxis - B0032 - SYFP2
311. pSB3T5 - B0032 - SYFP2
Result: Empty, new gel tomorrow.

Participant: Caroline, Katarina
Experiment: Transformation
225. pEL4C15 - J23106 - AAC(6') - linker - SYFP2 with 92. sRNA

Experiment: Restreak
202. pSB1K3 - B0034(mod) - LexA(G85D)
203. pSB1K3 - B0034(mod) - OmpF(rsr)
261. pSB3K3 - PT5lac - B0034 - LexA(G85D)

Experiment: Screening
251. pEL3C18 - J23116 - tetA - lacIs
300. pEL3C18 - J23116 - CTX-M - linker - lacIs
316. pEL3A18 - J23110 - CTX-M - SYFP2
317. pEL3A18 - J23110 - tetA - SYFP2
Result: All but 251 had good clones.

Experiment: O/N
300. pEL3C18 - J23116 - CTX-M - linker - lacIs
316. pEL3A18 - J23110 - CTX-M - SYFP2
317. pEL3A18 - J23110 - tetA - SYFP2

Experiment: Phusion PCR
178. pEL3C18 - J23116 - AAC(6') - linker - lacIs
179. pEL3C18 - PlacIQ - AAC(6')- linker - lacIs
219. pEL3C18 - J23106 - tetA - linker - lacIs
220. pEL3C18 - J23106 - AAC(6') - linker - lacIs
221. pEL3C18 - PlacIQ - CTX-M - linker - lacIs
222. pEL3C18 - PlacIQ - tetA - linker - lacIs
225. pEL4C15 - J23106 - AAC(6') - linker - SYFP2
257. pEL4C15 - J23110 - AAC(6') - linker - SYFP2
228. pEL4C15 - PlacIQ - CTX-M - linker - SYFP2
259. pEL4C15 - J23106 - CTX-M - linker - SYFP2
260. pEL3C18 - J23106 - CTX-M - linker - lacIs

Participant: Hampus
Experiment: Transformation
sRNA

Experiment: Restreak
206. pSB3T5 - J23101 - B0034 - OmpF(rsr)
207. pSB3Y5 - J23110 - B0034 - OmpF(rsr)

Experiment: Assembly
193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - T0
318 - pEL3S16 - Plac - B0034 - SYFP2 - AmpR - T0

Monday 27 August

Participant: Joel, Fredrik
Experiment: Screening
301. pSB3T5 - Plac - B0032 - SYFP2
302. pSB3T5 - PlaciQ - B0032 - SYFP2
303. pSB3T5 - PT5lac - B0032 - SYFP2
304. pSB3T5 - PLlacO - B0032 - SYFP2
305. pSB3T5 - J23101 - B0032 - SYFP2
306. pSB3T5 - J23106 - B0032 - SYFP2
307. pSB3T5 - J23110 - B0032 - SYFP2
308. pSB3T5 - J23116 - B0032 - SYFP2
309. pSB3T5 - Ptac - B0032 - SYFP2
310. pSB3T5 - Pxis - B0032 - SYFP2
311. pSB3T5 - B0032 - SYFP2
Result: All had good clones.

Participant: Fredrik
Experiment: Restreak
193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0
318. pEL3S16 - Plac - B0034 SYFP2 - AmpR - TT0

Participant: Joakim
Experiment: O/N
256. N3T

Participant: Julia, Hampus
Experiment: Plasmid prep.
164. pSB3T5 - Pxis - B0034 - SYFP2

Tuesday 28 August

Participant: Hampus, Julia
Experiment: Screening
193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0
Result: Good.

Experiment: Restreak
193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0

Experiment: O/N
164. pSB3T5 - Pxis - B0034 - SYFP2

Participant: Sabri
Experiment: Transformation
225+92 (reporter system + sRNA)
257+92 (reporter system + sRNA)
225+79 (to measure fitness cost for carrying two plasmids)
257+79 (to measure fitness cost)

Participant: Olle
Experiment: Screening
87. pSB1C3 - pBad
Result: All bands wrong.

Participant: Joel, Karl
Experiment: Mutagenesis, screening and PCR purification
178. pEL3C18 - J23116 - AAC(6') - linker - lacIs
179. pEL3C18 - PlacIQ - AAC(6')- linker - lacIs
219. pEL3C18 - J23106 - tetA - linker - lacIs
220. pEL3C18 - J23106 - AAC(6') - linker - lacIs
221. pEL3C18 - PlacIQ - CTX-M - linker - lacIs
222. pEL3C18 - PlacIQ - tetA - linker - lacIs
260. pEL3C18 - J23106 - CTX-M - linker - lacIs
Screening: Gel looked OK, but the concentration after the PCR purification gave negative results.

Participant: Joakim
Experiment: Screening
202. pSB1K3 - B0034(mod) - LexA(G85D)
261. pSB3K3 - PT5lac - B0034 - LexA(G85D)
Result: No good bands.

Experiment: Viability test of 256. MG1655/N3T

Wednesday 29 August

Participant: Joel
Experiment: Screening
178. pEL3C18 - J23116 - AAC(6') - linker - lacIs
179. pEL3C18 - PlacIQ - AAC(6')- linker - lacIs
219. pEL3C18 - J23106 - tetA - linker - lacIs
220. pEL3C18 - J23106 - AAC(6') - linker - lacIs
221. pEL3C18 - PlacIQ - CTX-M - linker - lacIs
222. pEL3C18 - PlacIQ - tetA - linker - lacIs
260. pEL3C18 - J23106 - CTX-M - linker - lacIs
320. pSB1C3 - pT5Imm
Result: Gel showed no bands.

Participant: Joakim
Experiment: Transformation
M13mp18

Experiment: O/N
MG1655 - pSa
MG1655 - R1drd19
MG1655 (cipro resistant)

Experiment: Counting colonies for the conjugation test

Thursday 30 August

Participant: Fredrik
Experiment: Screening and O/N
202. pSB1K3 - B0034(mod) - LexA(G85D)
206. pSB3T5 - J23101 - B0034 - OmpF(rsr)
261. pSB3K3 - PT5lac - B0034 - LexA(G85D)

Participant: Joel, Karl, Julia, Hampus
Experiment: Screening and O/N
305. pSB3T5 - J23101 - B0032 - SYFP2

Experiment: Mutagenesis
178. pSB3C18 - J23116 - AAC(6') - linker - lacIs
179. pSB3C18 - PlacIQ - AAC(6')- linker - lacIs
219. pEL3C18 - J23106 - tetA - linker - lacIs
220. pEL3C18 - J23106 - AAC(6') - linker - lacIs
221. pEL3C18 - PlacIQ - CTX-M - linker - lacIs
222. pEL3C18 - PlacIQ - tetA - linker - lacIs
260. pEL3C18 - J23106 - CTX-M - linker - lacIs

Experiment: Digestion with notI and screening
302. pSB3T5 - placiQ - B0032 - SYFP2
303. pSB3T5 - pT5lac - B0032 - SYFP2
304. pSB3T5 - PLlacO - B0032 - SYFP2
305. pSB3T5 - J23101 - B0032 - SYFP2
306. pSB3T5 - J23106 - B0032 - SYFP2
307. pSB3T5 - J23110 - B0032 - SYFP2
308. pSB3T5 - J23116 - B0032 - SYFP2
309. pSB3T5 - ptac - B0032 - SYFP2
310. pSB3T5 - pxis - B0032 - SYFP2
Result: Bad gel.

Participant: Julia, Hampus
Experiment: PCR purification
RFP

Experiment: Plasmid prep.
193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0

Participant: Joakim
Experiment: Conjugation test on 252+253 and 252+255

Friday 31 August

Participant: Joel
Experiment: Plasmid prep.
305. pSB3T5 - J23101 - B0032 - SYFP2

Experiment: Mutagenesis
317. pEL3A18 - J23110 - tetA - SYFP2

Experiment: PCR purification
178. pEL3C18 - J23116 - AAC(6') - linker - lacIs
179. pEL3C18 - PlacIQ - AAC(6') - linker - lacIs
219. pEL3C18 - J23106 - tetA - linker - lacIs
220. pEL3C18 - J23106 - AAC(6') - linker - lacIs

Participant: Hampus
Experiment: Cloning to 1C3
J23101 - Spot42
J23106 - AAC(6') - linker - SYFP2
Plac - B0034 - SYFP2
PT5lac - B0034
J23116 - AAC(6') - linker - lacIs

Participant: Johan
Experiment: Mutagenesis
pSB2K3 - tet inv. (H64A)
pSB2K3 - tet inv. (E147A)
pSB2K3 - tet inv. (H100A)
pSB3T5 - J23110 - tet inv. B0034 - SYFP2 (H64A)
pSB3T5 - J23110 - tet inv. B0034 - SYFP2 (E147A)
pSB3T5 - J23110 - tet inv. B0034 - SYFP2(H100A)

Participant: Julia
Experiment: Assembly
325. pSB1C3 - J23101 - Spot42
326. pSB1C3 - J23106 - AAC(6') - linker - SYFP2
327. pSB1C3 - Plac - B0034 - SYFP2 - AmpR - TT0

Participant: Joakim, Fredrik
Experiment: Preparation of competent cells
DA25015
DA3006

Saturday 1 September

Participant: Julia, Hampus
Experiment: Lambda red PCR
193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0

Sunday 2 September

Participant: Hampus
Experiment: Screening
262. pEL3K16 - PT5lac - B0034
325. pSB1C3 - Plac - B0034 - SYFP2
326. pEL3K16 - PT5lac - B0034
327. pSB1C3 - Plac - B0034 - SYFP2 - AmpR - TT0

Monday 3 September

Participant: Sabri
Experiment: Screening
262. pEL3K16 - PT5lac - B0034
325. pSB1C3 - J23101 - Spot42
326. pSB1C3 - J23106 - AAC(6') - linker - SYFP2
327. pSB1C3 - Plac - B0034 - SYFP2 - AmpR - TT0

Participant: Caroline, Katarina, Joel
Experiment: PCR purification, DpnI treatment, PCR purification
193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0
221. pEL3C18 - PlacIQ - CTX-M - linker - lacIs
222. pEL3C18 - PlacIQ - tetA - linker - lacIs
260. pEL3C18 - J23106 - CTX-M - linker - lacIs

Participant: Assembly
331. pEL3A18 - J23110 - tetA - linker - SYFP2

Participant: Hampus
Experiment: Isolation of non-fluorescent sRNA

Tuesday 4 September

Participant: Caroline, Katarina, Joel, Emy
Experiment: Assembly and transformation
336. pSB3S5 - Plac - B0032 - SYFP2
337. pSB3S5 - PlaciQ - B0032 - SYFP2
338. pSB3S5 - T5lac - B0032 - SYFP2
339. pSB3S5 - PLlacO - B0032 - SYFP2
340. pSB3S5 - J23101 - B0032 - SYFP2
341. pSB3S5 - J23106 - B0032 - SYFP2
342. pSB3S5 - J23110 - B0032 - SYFP2
343. pSB3S5 - J23116 - B0032 - SYFP2
344. pSB3S5 - Ptac - B0032 - SYFP2
345. pSB3S5 - Pxis - B0032 - SYFP2

Experiment: Transformation
331. pEL3A18 - J23110 - tetA - linker - SYFP2

Experiment: Lambda red into DA24100 and DA26250
193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0

Participant: Hampus
Experiment: Lambda red PCR
180. pEL3A18 - J23106 - AAC(6') - linker - SYFP2
213. pEL3A18 - J23110 - AAC(6') - linker - SYFP2

Wednesday 5 September

Participant: Olle
Experiment: O/N
349. pJNEG04 - miniF - SYFP2 - CTX-M

Participant: Anton
Experiment: Screening
332. pEL4T15 - PLlacO - B0034 - TAL2
334. pSB3K3 - PT5lac - B0034 - TAL1

Experiment: Restreak
346. pSB1K3 - B0034 - TAL2

Participant: Hampus
Experiment: Transformation, plasmid prep.
sRNA
Spot42

Participant: Joel, Anna, Karl
Experiment: Transformation
280. pEL4A15Iq - pUC - red
281. pEL4C15Iq - pUC - red
283. pEL4S15Iq - pUC - red
284. pEL4T15Iq - pUC - red
285. pEL4A15(FRT)Iq - pUC - red
286. pEL4C15(FRT)Iq - pUC - red
287. pEL4K15(FRT)Iq - pUC - red
288. pEL4S15(FRT)Iq - pUC - red

Experiment: Restreak
331. pEL3A18 - J23110 - tetA - linker - SYFP2
336. pSB3S5 - Plac - B0032 - SYFP2
337. pSB3S5 - PlaciQ - B0032 - SYFP2
338. pSB3S5 - T5lac - B0032 - SYFP2
339. pSB3S5 - PLlacO - B0032 - SYFP2
340. pSB3S5 - J23101 - B0032 - SYFP2
341. pSB3S5 - J23106 - B0032 - SYFP2
342. pSB3S5 - J23110 - B0032 - SYFP2
343. pSB3S5 - J23116 - B0032 - SYFP2
344. pSB3S5 - Ptac - B0032 - SYFP2
345. pSB3S5 - Pxis - B0032 - SYFP2

Thursday 6 September

Participant: Joel
Experiment: Screening
331. pEL3A18 - J23110 - tetA - linker - SYFP2
336. pSB3S5 - Plac - B0032 - SYFP2
337. pSB3S5 - PlaciQ - B0032 - SYFP2
338. pSB3S5 - T5lac - B0032 - SYFP2
339. pSB3S5 - PLlacO - B0032 - SYFP2
340. pSB3S5 - J23101 - B0032 - SYFP2
341. pSB3S5 - J23106 - B0032 - SYFP2
342. pSB3S5 - J23110 - B0032 - SYFP2
343. pSB3S5 - J23116 - B0032 - SYFP2
344. pSB3S5 - Ptac - B0032 - SYFP2
345. pSB3S5 - Pxis - B0032 - SYFP2
Result: Screening failed.

Experiment: Restreak
193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0

Participant: Sabri, Lisa, Hampus
Experiment: Transformation, plasmid prep
257. pEL4C15 - J23110 - AAC(6') - linker - SYFP2

Experiment: Restreak
non-fluorescent sRNA clones

Experiment: Mutagenesis
24. pSB3T5 - J23106 - tet inv. - B0034 - SYFP2
226. pSB3T5 - J23106 - tet inv. - B0034 - SYFP2
227. pSB3T5 - J23110 - tet inv. - B0034 - SYFP2

Friday 7 September

Participant: Hampus
Experiment: Screening
326. pSB1C3 - J23106 - AAC(6') - linker - SYFP2
327. pSB1C3 - Plac - B0034 - SYFP2 - AmpR - TT0
348. pSB1C3 - J23116 - AAC(6') - linker - lacIs

Participant: Hampus, Sabri
Experiment: Transformation
Randomized sRNA

Experiment: Restreak
Isolated sRNAs

Experiment: O/N
pSB1C3 - J23101 - Spot42

Participant: Joakim
Experiment: Ligation
202. pSB1K3 - B0034(mod) - LexA(G85D)
206. pSB3T5 - J23101 - B0034 - OmpF(rsr)
207. pSB3Y5 - J23110 - B0034 - OmpF(rsr)
208. pSB3T5 - J23101 - B0034 - MarR(mut)
211. pSB3T5 - PLlacO - B0034 - LexA(G85D)
261. pSB3K3 - PT5lac - B0034 - LexA(G85D)

Participant: Olle
Experiment: Mutagenesis of red to redq on
280. pEL4A15Iq - pUC - red
281. pEL4C15Iq - pUC - red
283. pEL4S15Iq - pUC - red
285. pEL4A15(FRT)Iq - pUC - red
286. pEL4C15(FRT)Iq - pUC - red
287. pEL4K15(FRT)Iq - pUC - red

Saturday 8 September

Participant: Joel, Joakim
Experiment: Screening
193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0
lac:amp
Result: 193 showed only primer dimers, lac:amp had good clones.

Participant: Lisa, Sabri
Experiment: Transformation
sRNAs into MG1655
sRNAs into 225

Experiment: Prepare cell cultures for cell sorting using FACS

Participant: Joakim
Experiment: Transformation
202. pSB1K3 - B0034(mod) - LexA(G85D)
206. pSB3T5 - J23101 - B0034 - OmpF(rsr)
207. pSB3Y5 - J23110 - B0034 - OmpF(rsr)
208. pSB3T5 - J23101 - B0034 - MarR(mut)
211. pSB3T5 - PLlacO - B0034 - LexA(G85D)
261. pSB3K3 - PT5lac - B0034 - LexA(G85D)

Experiment: Screening
178. pEL3C18 - J23116 - AAC(6') - linker - lacIs
179. pEL3C18 - PlacIQ - AAC(6') - linker - lacIs
219. pEL3C18 - J23106 - tetA - linker - lacIs
220. pEL3C18 - J23106 - AAC(6') - linker lacIs
221. pEL3C18 - PlacIQ - CTX-M - linker - lacIs
222. pEL3C18 - PlacIQ - tetA - linker - lacIs
260. pEL3C18 - J23106 - CTX-M - linker - lacIs
Result: No bands visible.

Participant: Sabri
Experiment: Screening
24. pSB2K3 - tet inv.
226. pSB3T5 - J23106 - tet inv. - B0034 - SYFP2
227. pSB3T5 - J23110 - tet inv. - B0034 - SYFP2

Sunday 9 September

Participant: Olle
Experiment: Screening
pEL4-series
TALENs
Result: Backbones looked good, TALENs gave no bands.

Monday 10 September

Participant: Joel, Olle, Pikkei
Experiment: Assembly and transformation
336. pSB3S5 - plac - B0032 - SYFP2
337. pSB3S5 - placiQ - B0032 - SYFP2
338. pSB3S5 - T5lac - B0032 - SYFP2
339. pSB3S5 - PLlacO - B0032 - SYFP2
340. pSB3S5 - J23101 - B0032 - SYFP2
341. pSB3S5 - J23106 - B0032 - SYFP2
342. pSB3S5 - J23110 - B0032 - SYFP2
343. pSB3S5 - J23116 - B0032 - SYFP2
344. pSB3S5 - Ptac - B0032 - SYFP2
357. pSB4C5 - J23110 - B0032 - SYFP2
358. pSB4K5 - J23110 - B0032 - SYFP2
359. pEL4T15 - J23110 - B0032 - SYFP2
360. pEL4C15 - J23110 - B0032 - SYFP2

Experiment: Lambda red PCR
193. pEL3S17 - Plac - B0034 - SYFP2 - AmpR - TT0

Experiment: O/N
lac:amp

Participant: Anna, Fredrik
Experiment: Screening and O/N
202. pSB1K3 - B0034(mod) - LexA(G85D)
206. pSB3T5 - J23101 - B0034 - OmpF(rsr)
207. pSB3Y5 - J23110 - B0034 - OmpF(rsr)
208. pSB3T5 - J23101 - B0034 - MarR(mut)
211. pSB3T5 - PLlacO - B0034 - LexA(G85D)
261. pSB3K3 - PT5lac - B0034 - LexA(G85D)

Participant: Olle
Experiment: Assembly and transformation
333. pEL3K16iq - J23110 - B0034 - eforRed
241. pEL4S17iq - red
243. pEL4S17iq - J23110 - B0034 - eforRed

Tuesday 11 September

Wednesday 12 September

Participant: Olle, Karl, Anna, Joel
Experiment: Screening
336. pSB3S5 - plac - B0032 - SYFP2
337. pSB3S5 - placiQ - B0032 - SYFP2
338. pSB3S5 - T5lac - B0032 - SYFP2
339. pSB3S5 - PLlacO - B0032 - SYFP2
340. pSB3S5 - J23101 - B0032 - SYFP2
341. pSB3S5 - J23106 - B0032 - SYFP2
342. pSB3S5 - J23110 - B0032 - SYFP2
343. pSB3S5 - J23116 - B0032 - SYFP2
344. pSB3S5 - Ptac - B0032 - SYFP2
357. pSB4C5 - J23110 - B0032 - SYFP2
358. pSB4K5 - J23110 - B0032 - SYFP2
359. pEL4T15 - J23110 - B0032 - SYFP2
360. pEL4C15 - J23110 - B0032 - SYFP2

Participant: Joakim
Experiment: O/N
MG1655 225
MG1655 257
sRNA

Thursday 13 September

Participant: Hampus
Experiment: Screening
325. pSB1C3 - J23101 - Spot42
361. pSB1C3 - RFP - linker - SYFP2
362. pSB1C3 - J23116 - AAC(6') - linker - lacIs
Result: 325 and 361 gave good bands, 362 gave no bands at all.

Participant: Julia
Experiment: Transformation
sRNA

Participant: Emy, Caroline, Katarina
Experiment: Tranformation
336. pSB3S5 - Plac - B0032 - SYFP2
337. pSB3S5 - PlaciQ - B0032 - SYFP2
338. pSB3S5 - T5lac - B0032 - SYFP2
339. pSB3S5 - PLlacO - B0032 - SYFP2
340. pSB3S5 - J23101 - B0032 - SYFP2
341. pSB3S5 - J23106 - B0032 - SYFP2
342. pSB3S5 - J23110 - B0032 - SYFP2
343. pSB3S5 - J23116 - B0032 - SYFP2
344. pSB3S5 - Ptac - B0032 - SYFP2
345. pSB3S5 - Pxis - B0032 - SYFP2
357. pSB4C5 - J23110 - B0032 - SYFP2
358. pSB4K5 - J23110 - B0032 - SYFP2
359. pEL4T15 - J23110 - B0032 - SYFP2
360. pEL4C15 - J23110 - B0032 - SYFP2

Experiment: Restreak
336. pSB3S5 - Plac - B0032 - SYFP2
337. pSB3S5 - PlaciQ - B0032 - SYFP2
338. pSB3S5 - T5lac - B0032 - SYFP2
339. pSB3S5 - PLlacO - B0032 - SYFP2
340. pSB3S5 - J23101 - B0032 - SYFP2
341. pSB3S5 - J23106 - B0032 - SYFP2
342. pSB3S5 - J23110 - B0032 - SYFP2
343. pSB3S5 - J23116 - B0032 - SYFP2
344. pSB3S5 - Ptac - B0032 - SYFP2
345. pSB3S5 - Pxis - B0032 - SYFP2
360. pEL4C15 - J23110 - B0032 - SYFP2

Friday 14 September

Participant: Katarina, Caroline, Joel, Emy
Experiment: Mutagenesis
215. pEL3A18 - PlacIQ - AAC(6') - linker - SYFP2
216. pEL3A18 - PlacIQ – tetA - linker - SYFP2
218. pEL3A18 - J23106 - tetA - linker - SYFP2
347. pEL3C18 - J23116 - tetA - linker - lacIs

Experiment: Screening and O/N
336. pSB3S5 - Plac - B0032 - SYFP2
337. pSB3S5 - PlaciQ - B0032 - SYFP2
338. pSB3S5 - T5lac - B0032 - SYFP2
339. pSB3S5 - PLlacO - B0032 - SYFP2
340. pSB3S5 - J23101 - B0032 - SYFP2
341. pSB3S5 - J23106 - B0032 - SYFP2
342. pSB3S5 - J23110 - B0032 - SYFP2
343. pSB3S5 - J23116 - B0032 - SYFP2
344. pSB3S5 - Ptac - B0032 - SYFP2
345. pSB3S5 - Pxis - B0032 - SYFP2
360. pEL4C15 - J23110 - B0032 - SYFP2

Participant: Julia
Experiment: O/N
MG1655 with 225. pEL4C15 - J23106 - AAC(6') - linker - SYFP2
MG1655 with 257. pEL4C15 - J23110 - AAC(6') - linker - SYFP2

Experiment: Plasmid prep.
86. pSB1A2 - PLlacO
361. pSB1C3 - RFP - linker - SYFP2
362. pSB1C3 - J23116 - AAC(6') - linker - lacIs

Saturday 15 September

Participant: Julia
Experiment: Restreak, plasmid prep.
sRNAs

Experiment: Screening, PCR purification and digestion with DpnI
AC6 - Spot42

Friday 21 September

Participant: Joel
Experiment: Restreak
215. pEL3A18 - PlacIQ - AAC(6') - linker - SYFP2
216. pEL3A18 PlacIQ – tetA - linker - SYFP2
218. pEL3A18 J23106 - tetA - linker - SYFP2
382. pSB4K5 - J23101 - B0032 - SYFP2
383. pEL4T15 - J23101 - B0032 - SYFP2
384. pEL8C15 - J23101 - B0032 - SYFP2

Experiment: Streak from frozen stock
336. pSB3S5 - plac - B0032 - SYFP2
337. pSB3S5 - placiQ - B0032 - SYFP2
338. pSB3S5 - T5lac - B0032 - SYFP2
339. pSB3S5 - PLlacO - B0032 - SYFP2
340. pSB3S5 - J23101 - B0032 - SYFP2
341. pSB3S5 - J23106 - B0032 - SYFP2
342. pSB3S5 - J23110 - B0032 - SYFP2
343. pSB3S5 - J23116 - B0032 - SYFP2
344. pSB3S5 - ptac - B0032 - SYFP2
345. pSB3S5 - pxis - B0032 - SYFP2

Team:Uppsala University/Notebook

From 2012.igem.org

Notebook

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