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From 2012.igem.org

Protocols  |  Single Gene Plasmids and Enzyme Characterisation  |  Showcasing Polysaccharide Production  |  The 3-Gene Inducible Plasmid Operon Construction  |  Glycobase

Protocols

Transformation Protocol from Invitrogen, for Top10 Competent Cells Transferring Cultures to Liquid Medium Mini Prep New England BioLabs Biobrick Assembly Protocol Annealing Oligomer Primers Biobrick Extraction Two-Step PCR Bacterial Genomic DNA Extraction Using GenElute PCR purification using QIAquick Linearized Plasmid Backbone Digestion and Ligation Protocol Preparation of IDTE Buffer and IDT Re-Suspension 3-Step PCR SDS-PAGE Protocol