Team:Peking/Modeling/Luminesensor/Orthogonality

Orthogonal Test in silico

To modularize the genetic system, our Luminesensor is expected to be bio-orthogonal with the origin system in bacteria. LexA, a natural element from the lactin-SOS system in bacteria may cause unexpected crosstalk. In order to remove this obstacle on the application prospects of our Luminesensor, we use LexA408 instead of the wild-type LexA. LexA408 and LexA are bio-orthogonal with each other since the sequence of the binding sites have variations (See Characterization).

Figure 10. Moecular Modeling for Luminesensor LexA408-VVD.

By adding several nodes into the network, we constructed modeling for orthogonality test:

Figure 11. Kinetic Network for Orthogonal Analysis

where

• L denotes Luminesensor
• I denotes the inner wild LexA
• D_L denotes the specific DNA binding site to Luminesensor
• D_I denotes the specific DNA binding site to wild LexA

The parameters are estimated as following:

Figure 12. Orthogonal Test Result.

The result shows that the contrast is highly related to the orthogonality. As our Luminesensor is orthogonal to the endogerous LexA system, our system still works well in bacteria with endogenous LexA (See Characterization).